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With the goal of developing an analytical method for the fast analysis of vitamins in a complex matrix, the authors created a method that used in-line and complementary HPLC with photodiode-array and MS detection techniques.

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Acoustic ejection mass spectrometry (AEMS) has recently emerged as the premier ultrahigh-throughput mass spectrometric methodology for drug discovery and related fields.

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This GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.

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The method of standard additions (MoSA) enables quantification of volatile impurities in condensed-phase samples, such as emulsions, for which matrix-matched calibration standards are required. The technique is, however, expensive because it requires multiple analyses of each sample.

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Extractable and leachable (E&L) testing for finished pharmaceutical products, bioprocess manufacturing systems, and medical devices is currently a subject of intense interest. The majority of the challenges encountered in analytical workflows are related to the highly complex matrices and relatively low analyte evaluation thresholds seen in E&L studies. This paper provide options for executing E&L testing to supporting regulatory submissions. There are multiple compliant routes possible, and the presented workflows and analytical solutions are only one of multiple successful approaches.

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This method greatly facilitates the analysis of a large number of pesticides.

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This article presents a new hydrophilic SAX hybrid monolithic column prepared by in‑capillary coating 5 μm bare silica particles with functional organic polymers. After preparation conditions were investigated and selected, the resulting column was characterized in detail.

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This work focuses on the effects of the mobile phase pH and the counterion concentration in buffer solution on retention in hydrophilic interaction liquid chromatography (HILIC) mode.

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This article explores what this transition to newer technology looks like, with an example applied to determining organic impurities of pramipexole dihydrochloride.

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In this column, we discuss the label-free and stable isotope labeling proteomics approaches that help in biomarker discovery. We also discuss the different enrichment techniques, such as stable isotope labeling by amino acid in cell culture (SILAC), isobaric tags for relative and absolute quantitation (iTRAQ), and tandem mass tags (TMT, that help in measuring low-abundance protein biomarkers.

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The authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.

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In this paper, Carsten Losch, the CEO of Berlin-based manufacturer of high-end scientific chromatography instruments – KNAUER – will tell you about the flexible integration of software in HPLC systems as well as giving you an outlook on where the chromatography industry is heading.

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When esterification occurs in your LC mobile phase, knowing how baseline quality, retention, and selectivity are affected will help you mitigate the effects.

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Recent advances in coating techniques and improved polymers employed as gas chromatographic stationary phases have resulted in commercializing low phase ratio capillary column technology with respectable chromatographic efficiency and inertness.

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In the present research, similar chromatography fingerprints were obtained using finely-tuned cryogenic-modulation (CM) and flow-modulation (FM) comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC–MS) experimental conditions.

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The Axcend FocusArray is the ideal choice for your challenging liquid chromatography requirements. The only diode array detectr for capillary HPLC offers a full three orders of magnitude linearity needed to identify trace elements in the presence of your target analytes.

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Learn 10 tips for dynamically creating cylinder-free accurate calibration gas standards for ppmv and lower concentrations.

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This paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.

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This article explores three case studies where autosampler injection programs help to (i) reduce the carry-over observed for large nucleic acids during anion-exchange chromatography (AEX), (ii) reduce peak distortion and breakthrough for monoclonal antibodies (mAbs) during hydrophilic-interaction chromatography (HILIC), and (iii) facilitate dissolution of lipid nanoparticles (LNPs) so that a size-exclusion chromatography (SEC) method can be applied for ribonucleic acid (RNA) payload analysis.

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The advantages of a risk-based approach to instrument replacement are discussed.

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New gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), continue to make progress in both primate and first-in-human trials. As this progress builds, the industry remains accountable for characterizing these molecules to meet the requirements of regulatory authorities.

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High-resolution mass spectrometry (HRMS) is an increasingly critical tool for identifying, characterizing, and monitoring attributes of protein-based therapeutics.

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Known as “forever chemicals”, PFAS exposure has been linked indirectly and directly to a range of potential health issues. The objective of this study was the development of an optimized sample preparation and LC–MS/MS method for the analysis of PFAS in serum for biomonitoring applications.

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Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.

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A UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.

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A main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.

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The investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.

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This article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.