Author
Latest:
LC–MS-Based Proteomics for Protein Biomarker Quantification for Both Prognosis and Diagnosis in the Clinical SettingIn this column, we discuss the label-free and stable isotope labeling proteomics approaches that help in biomarker discovery. We also discuss the different enrichment techniques, such as stable isotope labeling by amino acid in cell culture (SILAC), isobaric tags for relative and absolute quantitation (iTRAQ), and tandem mass tags (TMT, that help in measuring low-abundance protein biomarkers.
Latest:
Characterization of Styrene–Butadiene Block Copolymers by Gel Permeation ChromatographyThe authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.
Latest:
The evolving roles of chromatography softwareIn this paper, Carsten Losch, the CEO of Berlin-based manufacturer of high-end scientific chromatography instruments – KNAUER – will tell you about the flexible integration of software in HPLC systems as well as giving you an outlook on where the chromatography industry is heading.
Latest:
Chemistry in a Bottle: Ester Formation in Acidified Mobile-Phase SolventsWhen esterification occurs in your LC mobile phase, knowing how baseline quality, retention, and selectivity are affected will help you mitigate the effects.
Latest:
Low Phase Ratio Stationary Phase Column Technology for the Characterization of Highly Volatile and Reactive Compounds by Gas-Liquid ChromatographyRecent advances in coating techniques and improved polymers employed as gas chromatographic stationary phases have resulted in commercializing low phase ratio capillary column technology with respectable chromatographic efficiency and inertness.
Latest:
Cryogenic- and Flow-Modulation Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry: Obtaining Similar Chromatography PerformancesIn the present research, similar chromatography fingerprints were obtained using finely-tuned cryogenic-modulation (CM) and flow-modulation (FM) comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC–MS) experimental conditions.
Latest:
A New Era in HPLC: Introducing the Full-Stack Chromatography SystemWebinar Date/Time: Wed, Mar 19, 2025 11:00 AM EDT
Latest:
Tips for Cylinder-free Trace Gas CalibrationLearn 10 tips for dynamically creating cylinder-free accurate calibration gas standards for ppmv and lower concentrations.
Latest:
A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array DetectorThis paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.
Latest:
Advantages of Sequential or Bracketed Injection Methods to Improve the Chromatographic Analysis of BiotherapeuticsThis article explores three case studies where autosampler injection programs help to (i) reduce the carry-over observed for large nucleic acids during anion-exchange chromatography (AEX), (ii) reduce peak distortion and breakthrough for monoclonal antibodies (mAbs) during hydrophilic-interaction chromatography (HILIC), and (iii) facilitate dissolution of lipid nanoparticles (LNPs) so that a size-exclusion chromatography (SEC) method can be applied for ribonucleic acid (RNA) payload analysis.
Latest:
Application of a Risk‑Based Approach to the Migration of Validated Test MethodsThe advantages of a risk-based approach to instrument replacement are discussed.
Latest:
Advancing Gene Therapy: Enzyme Selection for Effective RNA Oligonucleotide MappingNew gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), continue to make progress in both primate and first-in-human trials. As this progress builds, the industry remains accountable for characterizing these molecules to meet the requirements of regulatory authorities.
Latest:
New Aspects in the Integration of MS Technologies in the Biopharmaceutical IndustryHigh-resolution mass spectrometry (HRMS) is an increasingly critical tool for identifying, characterizing, and monitoring attributes of protein-based therapeutics.
Latest:
A Comparative Study of Various Sample Preparation Approaches for PFAS in Biofluids: A Practical Method of Biomonitoring for Anthropogenic ToxicityKnown as “forever chemicals”, PFAS exposure has been linked indirectly and directly to a range of potential health issues. The objective of this study was the development of an optimized sample preparation and LC–MS/MS method for the analysis of PFAS in serum for biomonitoring applications.
Latest:
The Importance of Complete Overlapping of Analyte and Internal Standard Peaks in Eliminating Matrix Effects with Liquid Chromatography–Mass Spectrometry (LC–MS)Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.
Latest:
Rapid Simultaneous Determination of Five Major Alkaloids from Menispermi Rhizoma in Rat Urine by Ultrahigh-Pressure Liquid Chromatography– Tandem Mass Spectrometry (UHPLC–MS/MS) and its Application to a Urinary Excretion StudyA UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.
Latest:
Simple Tips to Increase Sensitivity in U/HPLC AnalysisA main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.
Latest:
A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS TransmissionThe investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
Latest:
Analysis of Coumarin in Tobacco, Smokeless Tobacco Products, and Electronic Cigarette Liquids by Isotope Dilution LC–MS/MSThis article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.
Latest:
Analysis of PFAS in Locally Acquired Food ContainersThe combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.
Latest:
Volatile Organic Compound and Sensory Profiles of Alcoholic Versus Non-Alcoholic Beer Using Immersive TF-SPME and SBSENon-alcoholic beers are becoming more popular and need to reflect the sophisticated flavourings of beers that are brewed containing alcohol. Sophisticated analytical techniques can help to achieve this.
Latest:
Chemistry in a Bottle: Ester Formation in Acidified Mobile-Phase SolventsWhen esterification occurs in your LC mobile phase, knowing how baseline quality, retention, and selectivity are affected will help you mitigate the effects.
Latest:
The LCGC Blog, Call for Abstracts! The 43rd Annual International Symposium and Exhibit on the Separation, Purification, and Characterization of Biologically Important Molecules!In this edition of the LCGC Blog, Jonathan Edelman discusses the 43rd Annual International Symposium and Exhibit on the Separation, Purification, and Characterization of Biologically Important Molecules (ISPPP) and provides a timeline for the upcoming conference.
Latest:
A Quick Approach to Screen Oligomers from Extractables Studies Using Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry (LC–QTOF-MS)Analysis of extractables and leachables (E&L) from plastic packaging is of great importance for pharmaceutical product safety. Accurate and rapid identification of unknown compounds in E&L is often complex and challenging. To address this challenge, we demonstrate a quick method for oligomer determination using LC–QTOF-MS.
Latest:
Fast HPLC Method Development Using Interfaced Photodiode-Array and Ion-Trap MS DetectionWith the goal of developing an analytical method for the fast analysis of vitamins in a complex matrix, the authors created a method that used in-line and complementary HPLC with photodiode-array and MS detection techniques.
Latest:
Method Development and Impurity Control in Late-Phase API ManufacturingOnce APIs move into later phases of development, an advanced impurity control strategy is vital and demands a comprehensive analytical methods approach.
Latest:
Automated FAMEs Analysis WorkbenchA flexible, automated workbench for determination of fatty acid content of a range of sample types, automating sample prep and analysis with a simple user interface.
Latest:
Separation Science: The State of the Art: The Future of Column Packing TechnologyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
Latest:
Detailed Glycosylation Analysis of Therapeutic Enzymes Utilizing Comprehensive 2D-LC–MSIn this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.
