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Application of a Risk‑Based Approach to the Migration of Validated Test MethodsThe advantages of a risk-based approach to instrument replacement are discussed.
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New Aspects in the Integration of MS Technologies in the Biopharmaceutical IndustryHigh-resolution mass spectrometry (HRMS) is an increasingly critical tool for identifying, characterizing, and monitoring attributes of protein-based therapeutics.
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Bioanalytical Analysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC–MS, Part 2: Sample PreparationThis concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.
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The Importance of Complete Overlapping of Analyte and Internal Standard Peaks in Eliminating Matrix Effects with Liquid Chromatography–Mass Spectrometry (LC–MS)Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.
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Rapid Simultaneous Determination of Five Major Alkaloids from Menispermi Rhizoma in Rat Urine by Ultrahigh-Pressure Liquid Chromatography– Tandem Mass Spectrometry (UHPLC–MS/MS) and its Application to a Urinary Excretion StudyA UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.
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Simple Tips to Increase Sensitivity in U/HPLC AnalysisA main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.
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A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS TransmissionThe investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
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Analysis of Coumarin in Tobacco, Smokeless Tobacco Products, and Electronic Cigarette Liquids by Isotope Dilution LC–MS/MSThis article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.
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Analysis of PFAS in Locally Acquired Food ContainersThe combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.
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Volatile Organic Compound and Sensory Profiles of Alcoholic Versus Non-Alcoholic Beer Using Immersive TF-SPME and SBSENon-alcoholic beers are becoming more popular and need to reflect the sophisticated flavourings of beers that are brewed containing alcohol. Sophisticated analytical techniques can help to achieve this.
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Chemistry in a Bottle: Ester Formation in Acidified Mobile-Phase SolventsWhen esterification occurs in your LC mobile phase, knowing how baseline quality, retention, and selectivity are affected will help you mitigate the effects.
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The LCGC Blog, Call for Abstracts! The 43rd Annual International Symposium and Exhibit on the Separation, Purification, and Characterization of Biologically Important Molecules!In this edition of the LCGC Blog, Jonathan Edelman discusses the 43rd Annual International Symposium and Exhibit on the Separation, Purification, and Characterization of Biologically Important Molecules (ISPPP) and provides a timeline for the upcoming conference.
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A Quick Approach to Screen Oligomers from Extractables Studies Using Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry (LC–QTOF-MS)Analysis of extractables and leachables (E&L) from plastic packaging is of great importance for pharmaceutical product safety. Accurate and rapid identification of unknown compounds in E&L is often complex and challenging. To address this challenge, we demonstrate a quick method for oligomer determination using LC–QTOF-MS.
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Fast HPLC Method Development Using Interfaced Photodiode-Array and Ion-Trap MS DetectionWith the goal of developing an analytical method for the fast analysis of vitamins in a complex matrix, the authors created a method that used in-line and complementary HPLC with photodiode-array and MS detection techniques.
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Method Development and Impurity Control in Late-Phase API ManufacturingOnce APIs move into later phases of development, an advanced impurity control strategy is vital and demands a comprehensive analytical methods approach.
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Automated FAMEs Analysis WorkbenchA flexible, automated workbench for determination of fatty acid content of a range of sample types, automating sample prep and analysis with a simple user interface.
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Separation Science: The State of the Art: The Future of Column Packing TechnologyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
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Detailed Glycosylation Analysis of Therapeutic Enzymes Using Comprehensive 2D-LC–MSIn this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.
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Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LCAnalyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
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Effectiveness of High-Resolution Ion Mobility for Complex AnalysesHRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
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Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
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Optimized Devices for Challenging Nanofluidic ApplicationsBiotech Fluidics recently released the Biotech Micro Flow Meter, a tool for continuously monitoring the consistency of nanoscale dispensing systems as well as UHPLC and LC/MS solvent/reagent delivery systems.
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Towards the Use of Multidimensional Gas Chromatography Techniques in Routine Analysis: The Mineral Oil CaseThere are several key benefits to using multidimensional GC techniques in routine analysis. An examination of mineral oil analysis in food reveals the utility of these techniques.
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Multiple-Cumulative Trapping: A Fascinating Option to Enhance Headspace ProfileMultiple-cumulative trapping headspace extraction coupled with high-concentration-capacity tools, such as SPME, enhances sensitivity of volatiles, as illustrated here in food analysis.
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Comparing the Flavour Profiles of Soft Drink Brands Using Immersive Sorptive Extraction, GC×GC–TOF-MS, and ChemometricsImmersive sorptive extraction coupled with comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC–TOF-MS) was used to compare flavour profiles from popular brand soft drinks with those of imitation products.
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Empower Your Business with the Empower Data Science Link***Live: Wednesday, March 31, 2021 at 10am EDT | 3pm BST | 4pm CEST*** Learn how Empower Data Science Link enables your digital transformation via our new cloud-based data platform approach to standardize your data, harmonize it , and make it actionable - to improve the overall chromatographic workflows across your organization. Improve productivity, compliance and overall efficiency in your lab with Empower Data Science Link powered by TetraScience. ***On demand available after final airing until Mar. 31, 2022***
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Six Key Differentiators Between Liquid Chromatography and High-Resolution Ion MobilityUsing ion mobility, analytes that have the same molecular mass can be separated by their shape, centers of mass, and collision cross section, but challenges such as ion loss can still occur. A new development in ion mobility separation, high-resolution ion mobility (HRIM), addresses such problems, and is particularly well suited to challenging applications, such as glycosylation monitoring of biological drugs and vitamin D analysis.
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Effectiveness of High-Resolution Ion Mobility for Complex AnalysesHRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
