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Permeation tubes are used in various applications for creating trace concentration gas standards for calibrating gas analytical devices such as a gas chromatograph.

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This paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.

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This article explores three case studies where autosampler injection programs help to (i) reduce the carry-over observed for large nucleic acids during anion-exchange chromatography (AEX), (ii) reduce peak distortion and breakthrough for monoclonal antibodies (mAbs) during hydrophilic-interaction chromatography (HILIC), and (iii) facilitate dissolution of lipid nanoparticles (LNPs) so that a size-exclusion chromatography (SEC) method can be applied for ribonucleic acid (RNA) payload analysis.

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The advantages of a risk-based approach to instrument replacement are discussed.

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High-resolution mass spectrometry (HRMS) is an increasingly critical tool for identifying, characterizing, and monitoring attributes of protein-based therapeutics.

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This concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.

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Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.

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A UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.

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A main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.

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The investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.

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This article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.

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The combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.

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Non-alcoholic beers are becoming more popular and need to reflect the sophisticated flavourings of beers that are brewed containing alcohol. Sophisticated analytical techniques can help to achieve this.

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When esterification occurs in your LC mobile phase, knowing how baseline quality, retention, and selectivity are affected will help you mitigate the effects.

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In this edition of the LCGC Blog, Jonathan Edelman discusses the 43rd Annual International Symposium and Exhibit on the Separation, Purification, and Characterization of Biologically Important Molecules (ISPPP) and provides a timeline for the upcoming conference.

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Analysis of extractables and leachables (E&L) from plastic packaging is of great importance for pharmaceutical product safety. Accurate and rapid identification of unknown compounds in E&L is often complex and challenging. To address this challenge, we demonstrate a quick method for oligomer determination using LC–QTOF-MS.

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With the goal of developing an analytical method for the fast analysis of vitamins in a complex matrix, the authors created a method that used in-line and complementary HPLC with photodiode-array and MS detection techniques.

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Once APIs move into later phases of development, an advanced impurity control strategy is vital and demands a comprehensive analytical methods approach.

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A flexible, automated workbench for determination of fatty acid content of a range of sample types, automating sample prep and analysis with a simple user interface.

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In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.

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In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

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Analyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.

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HRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.

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Errors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.

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Biotech Fluidics recently released the Biotech Micro Flow Meter, a tool for continuously monitoring the consistency of nanoscale dispensing systems as well as UHPLC and LC/MS solvent/reagent delivery systems.

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There are several key benefits to using multidimensional GC techniques in routine analysis. An examination of mineral oil analysis in food reveals the utility of these techniques.

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Multiple-cumulative trapping headspace extraction coupled with high-concentration-capacity tools, such as SPME, enhances sensitivity of volatiles, as illustrated here in food analysis.

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Immersive sorptive extraction coupled with comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC–TOF-MS) was used to compare flavour profiles from popular brand soft drinks with those of imitation products.