Author


Thomas Lauer

Latest:

Where Has My Efficiency Gone? Impacts of Extracolumn Peak Broadening on Performance, Part 4: Gradient Elution, Flow Splitting, and a Holistic View

In this instalment, we focus on the impact of elution mode (isocratic or gradient) and postcolumn flow splitting on the total level of extracolumn dispersion (ECD) in a liquid chromatography (LC) system, and demonstrate the use of a free, web-based calculator that can be used to guide decision making aimed at reducing ECD during method development.


Conner McHale

Latest:

Turn Down the Volume

Extra column volume is the volume in an HPLC system external to the column that contributes to the total peak volume, which will impact peak width, resolution, and overall efficiency.


Hunter J. Campbell

Latest:

Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During Brewing

A rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.


Stephan Baumann

Latest:

Comparing the Chemical Profiles of Plant-Based and Traditional Meats Using GC–MS-Based Metabolomics

Untargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.


Jana Rousova Hepner

Latest:

There is No Time to Waste: Low-Pressure Gas Chromatography– Mass Spectrometry is a Proven Solution for Fast, Sensitive, and Robust GC–MS Analysis

Low-pressure gas chromatography (LPGC)–MS should be the first option in many GC–MS applications to provide fast, sensitive, and robust analyses; and the installation, instrumentation, and implementation of LPGC is possible in any GC–MS system.


Erasmus Cudjoe

Latest:

Novel LC–MS/MS Method with a Dual ESI and APCI Ion Source for Analysis of California-Regulated Pesticides and Mycotoxins in Medium-Chain Triglyceride (MCT) Oil Cannabis Tinctures

A novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) source was developed for analysis of 66 pesticides and 5 mycotoxins regulated by the State of California in medium-chain triglyceride (MCT) oil cannabis tinctures. The limits of quantitation (LOQs) of all of the pesticides and mycotoxins were well below California state action limits of these analytes in cannabis-based non-inhalable or edible products. A total of 62 (out of 66) pesticides and 5 mycotoxins were analyzed using LC–MS/MS with an ESI source, and the remaining 4 pesticides were determined using LC–MS/MS with an APCI source. A simple, fast, and cheap acetonitrile solvent extraction method was used for sample preparation to get good recovery and achieve high throughput for this analysis. For late-eluted analytes, a number of internal standards were used to compensate for ion suppression effects from the hydrophobic matrix.




Ned E. Bibler

Latest:

Ion Chromatography Separation of Alkali, Alkaline Earth, Transition, Post-transition, Lanthanide, and Actinide Metal Cations in Waste Sludge

The authors developed an ion chromatography method to separate 31 cations in a single chromatographic run using inductively coupled plasma–mass spectrometry, liquid scintillation counting, and gamma counting. The high-level radioactive waste samples contained trace radionuclides in concentrations of only a few hundred disintegrations-per-minute-per-milliliter.


Christin Pleger

Latest:

Online Ion-Exchange Solid-Phase Extraction Cleanup for PFAS in Food of Animal Origin

Online SPE–LC–MS/MS combined with the method presented enabled automated cleanup of food extracts and determination of PFAS compounds in the ng/kg range.


Harold Weller

Latest:

A Full Scan Data Review Tool to Match the Speed of Acoustic Ejection Mass Spectrometry

Acoustic ejection mass spectrometry (AEMS) has recently emerged as the premier ultrahigh-throughput mass spectrometric methodology for drug discovery and related fields.


Kira Fisher

Latest:

Detangling the Complex Web of GC × GC Method Development to Support New Users

In this article, the authors describe a simple workflow to develop a GC×GC method for a specific sample upon initial use, with the aim of decreasing the time to accomplish functional workflows for new users.


Thomas D. Bucheli

Latest:

Suspect Screening of Lupin-Produced Phytotoxins in Environmental Samples

The authors introduce a new high-throughput approach for analyzing environmental micropollutants.


Ørjan G. Martinsen

Latest:

Electromembrane Extraction with Vials of Conducting Polymer

In electromembrane extraction (EME), the target analyte is extracted from an aqueous sample across a supported liquid membrane (SLM) and analyzed using LC. Through the study presented here, we demonstrate the principles of the technique and test its performance.


Katherine Keppel-Jones

Latest:

The Analysis of Low Molecular Weight Carboxylic Acids by CE with Indirect UV Detection

Capillary electrophoresis (CE) is a useful and versatile technique for a large number of applications. Devoting some discussion to the development of these reported methods to illustrate the separation approaches and strategies to obtain reproducible results, they also compare CE's advantages and disadvantages with those of the more typical method for each type of analysis.


Anas Kamleh

Latest:

Separation of Isomeric Metabolites and Gangliosides with High Performance (Drift Tube) Ion Mobility–Mass Spectrometry

Employing ion mobility at atmospheric pressure without vacuum enables coupling to high-resolution mass analyzers. High performance ion mobility–orbital trap mass spectrometry (HPIM-OT-MS) is a useful alternative for separating isomers, such as isomeric metabolites seen in drug discovery.


Stephen Norris

Latest:

Supplementary Info: Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates

This information is supplementary to the article “Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates” that was published in the March 2022 issue of LCGC Asia Pacific.


M. Montes-Bayon

Latest:

Towards Automated Routine Analysis of the Distribution of Trace Elements in Single Cells Using ICP-MS

Analysis of the compositional variation in living cells is essential for understanding biological processes. Single-cell elemental analysis by triple-quadrupole ICP-MS is emerging as a selective, highly sensitive, and potentially high-throughput technique for the study of constitutive elements, and uptake of metallodrugs (or metal-containing nanomaterials) in single cells.


Safni

Latest:

Ion Chromatography Using Anion Exchangers Modified with Anionic Polysaccharides

The authors demonstrate a new detection method for various anions and cations separated by ion chromatography and point out the main advantages of this method.


Fengpeng Zhu

Latest:

Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC Analysis

Errors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.


David O. Carter

Latest:

Investigating Decomposition Odor in a Tropical Climate by Comprehensive Two-Dimensional Gas Chromatography (GC×GC) Coupled with Mass Spectrometry (MS) and Flame Ionization Detection (FID)

Decomposing animal tissue releases volatile organic compounds (VOCs), of interest in forensic science. We describe the use of GC×GC–qMS/FID retrofitted with a reverse fill/flush (RFF) flow modulator for analyzing these VOCs in a tropical climate.



Brian Rohrback

Latest:

Controlling Retention Time Drift in Industrial Chromatography Settings

Computer-enabled technology approaches can mitigate the impact of aging instruments and instrument differences—leading to more robust, lower-maintenance analysis in industrial settings.


Prabhat Kumar Upadhyaya

Latest:

Validation of HPLC-DAD Based Stability Indicating Assay Method for Ornidazole in Periodontal Polymeric Hydrogel: Robustness Study using Quality by Design (QbD) Approach

In the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.


Pascal Steffen-Lockhauserbäumer

Latest:

Advances in Spatial Biology using TIMS-TOF MS

A look at the practical benefits of using TIMS-TOF MS in spatial biology applications.


Karen Sam

Latest:

CDS Analytical - Analysis of Tetrahydrocannabinol Vape Oils Using Pyroprobe by Thermal Extraction

This application note demonstrates a multistep thermal extraction study on tetrahydrocannabinol (THC) vape oils.



Amber Hupp

Latest:

The LCGC Blog: Career Advice for Aspiring Academics

In this edition of the LCGC Blog, Amber Hupp of the College of the Holy Cross provides advice for aspiring analytical chemists and shares accounts of other professionals.


C. Rimmer

Latest:

Development of a Standardized Protocol for the Classification of Column Sets in Comprehensive Two-Dimensional Gas Chromatography

The article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.


Abbey Fausett

Latest:

Novel GC–MS Software Programs for Analyzing Seized Drug Samples

The role of software to identify and distinguish seized drug samples.