Application Notes: General

Level up your Empower data with in-depth dashboards and powerful custom data analytics. waters_connect Data Intelligence Software provides real-time, in-depth dashboards and powerful, purpose-built analytics on your Empower Software data to reduce risk, save money, improve productivity, and accelerate time- to-market.

Discover how you can simplify the audit preparation process with data integrity dashboards that provide transparency to key actions, and seamlessly track long-term trends and patterns, helping to prevent system suitability failures before they occur with waters_connect Data Intelligence software.

PFAS testing can be highly challenging, which is why we make it easier with our convenient portfolio of PFAS reference materials. Advance environmental and health safety with standards you can trust.

Ensure environmental and consumer health with our standards for 6PPD-quinone testing. 6PPD-quinone has been detected in the environment and has shown toxicity to aquatic life. Chiron, by Zeptometrix® offers reference standards suitable for Draft EPA Method 1634.

This white paper explores the health risks, environmental impacts, and detection technologies associated with PFAS, along with the latest advancements in PFAS research. It also provides an overview of the regulatory landscape and emphasizes the crucial role of companies like ZeptoMetrix® in supplying PFAS reference materials, which are essential for ensuring the accuracy of testing. Lastly, the paper outlines key areas for future PFAS research.

The World’s First Microplastics Reference Materials. Our scientists have focused on these emerging global threats, and are excited to share Chiron MicroPrefs®, the first commercial microplastic reference material. The MicroPref® portfolio is designed to detect the six most abundant plastics in the environment and is available in a novel, easy-to-use tablet formulation. Be among the first labs to join in the fight against microplastic pollution by exploring the NEW line of Chiron MicroPrefs® microplastic standards.

Learn the latest techniques for improved chromatographic performance and throughput of AAV analysis.

In the development of protein-based drugs, deep analysis of glycan types and locations and high-throughput trending analysis of glycan profiles are crucial to maintaining product quality.

Per- and polyfluoroalkyl substances (PFAS) are exceptionally versatile chemicals and have seemingly endless applications. Due to their persistent nature; prevalence in blood, food and environment; and emerging toxicity, there is a significant concern for their presence in the human body. Analysis is especially complicated due to background contamination in laboratory supplies and system. This paper will discuss sample preparation and LC-MS/MS analysis of an expanded panel of PFAS compounds in serum.

Oligonucleotides—therapeutic agents for difficult-to-treat diseases—rely on precise molecular weight confirmation to ensure quality during development. Experts from Agilent Technologies explore the challenges of liquid chromatography-mass spectrometry (LC/MS) analysis in oligonucleotide synthesis, as well as strategies for accurate deconvolution, method development, and optimal equipment selection.

This application note examines the selectivity differences between three L1 columns. The CORTECS Premier C18, CORTECS Premier C18+ and CORTECS Premier T3 Columns were used to separated paracetamol and its impurities using EP monograph conditions.

In this application note, a USP monograph is modernized to a shorter column using 5 µm particles, which can be allowable under USP <621> as long as the N value is within the guidelines. By using highly efficient CORTECS 5 μm particles, this type of modernization is possible and reductions in solvent usage and run times are achieved.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

In this work the development of two key attributes of the 5um CORTECS Columns is examined. First column efficiency is compared across CORTECS and other solid-core 5 μm columns. Next scalability from sub-2 μm to 5 μm particles is examined between the CORTECS Column lines and competitive column lines. It was found that CORTECS columns have higher efficiency compared to other solid-core columns and that CORTECS particles are fully scalable.

Trace-level analysis of VOCs in a tomato product using headspace extraction with large volume preconcentration (LVP) and multi-step enrichment (MSE)

This study demonstrates the Centri® sample extraction and enrichment platform’s unique multi-step enrichment feature to enhance the discovery of trace-level, packaging-derived contaminants in food and beverage samples by GC–MS.

Efficient separation N-glycans and isomers by HPAEC and quantification with Pulsed Amperometric Detection (PAD) and MS for identification.

This study demonstrates highly sensitive detection of residual fumigants in seeds and spices using MSE–HS–trap with GC–MS, achieving detection limits as low as 0.0018 mg/kg, well below EU regulatory limits. Excellent linearity (R² > 0.99), reproducibility, and throughput (40 samples/day) are achieved. Enhanced sensitivity with single-ion-monitoring (SIM) is also demonstrated for ethylene oxide and 2-chloroethanol.

Various samples, ranging from short-chain FOS to long-chain inulin including heterogenous blends of inulin-type fructans, can be easily analyzed using HPAEC-PAD.

Separation of oligo- and polysaccharides in honey up to DP40 within 25 minutes run time, enabling fast profiling of fraudulent/adulterated honey.

Optimized method for oligo– and polysaccharides profiling by separating fructo– and maltooligosaccharides for honey adulterations (fraud) measurements.

Fast separation (within 8 min) and quantification of lactose and its isomers in commercial lactose-free products using HPAEC-PAD.

Comprehensive analysis of fermentable an non-fermentable sugars in beer using the ALEXYS™ Carbohydrate Analyzer equipped with SweetSep™ AEX200 column.

Fast and sensitive method for the quantification of sucralose within 8 min in beverages and chewing gum using HPAEC-PAD with SweetSep™ column.

Application note outlining the methodology for assessing significant levels of PFAS emissions from food contact materials under typical usage conditions. PFAS were detected across a range of temperatures for the items tested, with concentrations varying from 1 pg/g to over 6000 pg/g.

This study shows how GC–MS performance for the sampling of aroma compounds and off-odours in beverages can be enhanced by using techniques incorporating trap-based preconcentration. The first part of the study focuses on SPME, and how trapping and enrichment can improve peak symmetry, qualitative analysis and sensitivity. The second part of the study compares these methods against automated robe-based high-capacity sorptive extraction, which as well as being operationally robust, ffers improved recovery for higher-boiling compounds and an extended analyte range.

High-resolution separation of maltodextrin with outstanding linearity, repeatability, and sensitivity, enabling the precise quantification of its components.

LPGC-MS Multiresidue Pesticides Analysis

An extraction method with an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 HPLC column.

Evaporation enhances method sensitivity. This study investigates the impact of gas temperature and flow rate on blowdown evaporation of solvents and their mixtures