Biopharmaceuticals and Protein Analysis

Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.

Politecnico di Milano scientists recently created a new optimization strategy for chromatographically purifying oligonucleotides.

Michael G. Bartlett of the University of Georgia looked at how hydrophilic interaction liquid chromatography (HILIC) has evolved for oligonucleotide analysis in a new article.

Scientists recently used thermal desorption (TD) and gas chromatography– time-of-flight mass spectrometry (GC×GC-TOFMS) to track per- and polyfluoroalkyl substances (PFAS) emissions from materials used in firefighter gear.

Researchers from Bristol Myers Squibb and Sanofi recently tested a new means of using hydrophobic interaction chromatography (HIC) for removing impurities in monoclonal antibodies (mAbs).

Northwest University and Shaanxi Institute for Food and Drug Control scientists recently tested a new means of separating bioactive compounds from herbal extracts.

Navin Rauniyar and Xuemei Han of Tanvex Biopharma USA recently discussed how identifying product-related variants through characterization enables the recognition of impurities that compromise the quality and safety of drugs.

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.

Separation and Characterization of Macromolecules 11 (SCM-11) will take place from 22–24 January 2025 in Amsterdam, The Netherlands.

As part of our ISC 2024 coverage, we recently interviewed Alexandre Goyon of Genentech about his research and his being awarded our Rising Stars of Separation Science Award for Outstanding Achievement in Pharmaceutical Analysis.

As part of our ISC 2024 coverage, we recently interviewed Amarande Murisier of the University Hospital of Lausanne, Switzerland (CHUV) about her winning the Rising Stars of Separation Science Award for Biopharmaceutcal Analysis and her scientific background.

Amarande Murisier has earned recognition in the field of biopharmaceutical separation sciences with method development of SEC and IEC hyphenated with MS.

The 34th International Symposium on Chromatography kicked off on October 6 in Liverpool, featuring two plenary sessions and an opening ceremony.

MS-SMART integrates three intelligent algorithms: automatic mining of diagnostic ions, rapid filtration of alkaloids from untargeted MS/MS data, and structural recommendations for filtered components.

Scientists from Tianjin universities and Cleveland State University investigated how to characterize a potent chemical compound in Platycodon grandiflorus roots.

Here is some of the most popular content posted on LCGC International this week.

Hangzhou Dianzi University scientists recently tested a new metal–organic framework (MOF)-based system for extracting benzodiazepines (BZPs) from urine.

Soochow University scientists recently reviewed how HILIC breakthroughs have helped with handling glycopeptides and glycans.

Scientists from Politecnico di Milan in Milano, Italy created a new optimization approach for chromatographically purifying oligonucleotides.

Karlstad University scientists modeled pH-dependent retention and competitive adsorption of solutes using mixed-mode and reversed-phase liquid chromatography.

Lausanne University Hospital scientists recently tested ultrahigh-performance supercritical fluid chromatography hyphenated to tandem mass spectrometry (UHPSFC–MS/MS) for detecting urinary endogeneous steroids.

Waters Corporation scientists recently examined how different mobile and stationary phases affect small interfering RNA (siRNA) duplex stability in liquid chromatography.

Scientists from Shanghai Jiao Tong University and the Sichuan China Tobacco Industry Company used gas chromatography-ion mobility spectrometry (GC-IMS) to analyze the flavor profiles of different cigars.

Scientists from Universidad Autónoma del Estado de Hidalgo recently tested a new dispersive solid phase micro-extraction (DSPME) technique for removing naphthol isomers from water samples.

National Institute of Pharmaceutical Education and Research (NIPER) scientists recently highlighted LC–MS’s use in improving drug modalities.

Scientists from the Swedish university analyzed the different ways water and trifluoroacetic acid (TFA) affect peptides in sub- and supercritical fluid chromatography (SFC) separations.

Steven Ray Wilson and Hanne Røberg-Larsen of the University of Oslo recently discussed the practical application and advantages of their FDA-validated research to analyse small molecule drugs and their metabolites in organoid/organ-on-chip systems that serve as alternatives to animal models.

Mankind Research Centre scientists recently tested a new solid phase extraction (SPE)-based approach coupled with GC–MS for analyzing small molecule N–nitrosamine impurities in antitussive syrups.

Here are the top five articles published on LCGC International this week.