Application Notes: LC

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple efficient identification of the whole vitamin series (K2, K3, and K4).

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This application note demonstrates the potential of valorizing mother liquor using Centrifugal Partition Chromatography (CPC) to remediate minor cannabinoids.

This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.

In this application note the chelating agents 8-hydroxyquinoline and hinokitiol were analyzed using a bioinert coated YMC-Accura Triart C18 column.

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

A new technical study demonstrates the utility of a non-invasive liquid flowmeter for validating the performance of HPLC systems across a range of 9 different solvents.

We assess the benefits of the Alliance iS Bio HPLC System by migrating and modernizing a compendial SEC method outlined in the USP General Chapter <129>.

Combining the gradient method adjustments with the Alliance™ iS HPLC System to achieve both column dimension and system modernization for the USP monograph separation of abacavir sulfate

We show the development of HPLC-ELSD methods for high-throughput and sensitive analysis of the lipids in lipid nanoparticles (LNPs), used to administer mRNA vaccines.

The traditional method for HPLC gradient validation uses a UV detector which has documented limitations. An improved approach using non-invasive flowmeters is described.

This brief communication highlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

Despite the utility of the essential oil mixture, it has been shown that allergies can arise from some of the individual components.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.

Chromium can pose as a beneficial element or a poison, therefore it is imperative to identify contaminated waters, especially drinking water.

GRPC peptides are found in the entire body. Isolation of seven classic GRPC peptides initiating in the GI tract could implicate disease.

The isolation and identification of 5 common psychedelic compounds was performed using the alkaline ion-pairing agent hexylamine to facilitate excellent resolution.

This note summarizes the optimal methods for the HPLC separation of semaglutide and liraglutide under acidic and alkaline conditions.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

We show the development of HPLC-ELSD methods for high-throughput and sensitive analysis of the lipids in lipid nanoparticles (LNPs), used to administer mRNA vaccines.

Recycling chromatography is a powerful technique that allows the separation of two closely eluting peaks by increasing the column bed length.