HILIC

Here is some of the most popular content posted on LCGC International this week.

Scientists recently studied how different factors affect antisense oligonucleotides (ASOs) under hydrophilic interaction liquid chromatography (HILIC) conditions.

Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.

Here is some of the most popular content posted on LCGC International this week.

Michael G. Bartlett of the University of Georgia looked at how hydrophilic interaction liquid chromatography (HILIC) has evolved for oligonucleotide analysis in a new article.

Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.

Here is some of the most popular content posted on LCGC International this week.

Soochow University scientists recently reviewed how HILIC breakthroughs have helped with handling glycopeptides and glycans.

Karlstad University scientists modeled pH-dependent retention and competitive adsorption of solutes using mixed-mode and reversed-phase liquid chromatography.

Waters Corporation scientists recently examined how different mobile and stationary phases affect small interfering RNA (siRNA) duplex stability in liquid chromatography.

Here are the top five articles that the editors of LCGC International published this week.

Scientists from the University of Geneva in Geneva, Switzerland and AstraZeneca in Mölndal analyzed how HILIC is related to the connections between oligonucleotide structures and diastereomer separation.

University of Tübingen scientists recently tested a new approach to hydrophilic-interaction chromatography (HILIC) to address retention time repeatability issues.

In the world of liquid chromatography, innovative strides in column technology continue to take place. We are also reminded that there is always more to learn about “well-known” methodologies, and our craft is continuously influenced by important social concerns.

This work focuses on the effects of the mobile phase pH and the counterion concentration in buffer solution on retention in hydrophilic interaction liquid chromatography (HILIC) mode.

LCGC’s Senior Technical Editor uses artificial intelligence (AI) platform ChatGPT to answer questions about the role of AI in separation science. Do you agree with ChatGPT?

Here are the top five articles that the editors of LCGC International published this week.

This article covers liquid chromatography columns and accessories commercially released after Pittcon 2023 through the 2024 conference.

The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.

At Pittcon 2024, LCGC International editor Patrick Lavery sat down with Wolfgang Lindner of the University of Vienna to talk about his career in liquid chromatography (LC).

The process was assisted by pH-dependent acid-base partitioning coupled with salting-out-assisted liquid-liquid extraction (LLE).

A variety of chromatographic techniques have been used to quantify the full range of acyl-CoAs, formed when a fatty acid bonds with coenzyme A, but added complexity and analysis time adversely affect the level of throughput.

The Chromatographic Society will honor five scientists and academics next year, conferring its highest honor, the Martin Medal, to David McCalley and Fabrice Gritti.

A comprehensive analytical approach utilizing online two-dimensional liquid chromatography coupled with hybrid linear ion trap orbital trap mass spectrometry, has enabled the in-depth analysis of Huangqi Guizhi Wuwu decoction, identifying 170 chemical constituents and offering insights into its complex chemical composition and potential therapeutic properties.

This instalment of “Column Watch” presents many of the highlighted topics and trends observed at this exceptionally well-run symposium.

Increases and decreases in anthocyanin concentration, which relates to the level of sugars found in red wine, were observed at specific weekly intervals of the grape ripening process.

An overview of different approaches for the qualitative and quantitative analysis of polysorbates.

A team of researchers has investigated strategies to overcome sample introduction challenges in hydrophilic-interaction chromatography (HILIC). By employing techniques such as pre-column dilution and selecting alternative organic solvents, they successfully addressed the issues related to injection solvent strength, resulting in improved peak symmetry and separation efficiency in HILIC analyses.

In a presentation at HPLC 2023, Kevin Pagel of Freie Universität in Berlin, Germany described methods he has found most effective for better understanding the roles of glycans and glycoconjugates in biological processes.

Davy Guillarme of the University of Geneva said in a talk at HPLC 2023 that short columns have shown promise, but ultrahigh-performance liquid chromatography (UHPLC) instrumentation is not yet optimized to realize their benefits.