Application Notes: Size-Exclusion Chromatography (SEC)

Switching the GPC/SEC solvent from THF to 2-methyl-THF provides an easy swap to provide a greener, safer, and less toxic option.

This technical note demonstrates the SEC-based separation and quantification of Cas9 IVT mRNA (with a total length of approximately 4500 nucleotides) and its aggregates.

SEC data demonstrates mRNA aggregate analysis for vaccine stability. Optimal separation occurs at 100 mM NaCl and low flow rates, enhancing mRNA production quality.

The data show that a separation between circular and linear plasmid DNA is possible and the performed linearization can be monitored using SEC-HPLC.

This application showcases the utility of SEC to resolve s.s. oligonucleotides from duplexes in mixtures of four oligonucleotide lengths (21, 40, 80, and 100mers).

Biozen dSEC-7 accurately resolves AAV serotypes 5, 8 and 9 monomer from aggregates in under 10 minutes with consistent results across different mobile phase conditions.

This application note demonstrates the performance of BioCore SEC columns for determining aggregation and fragmentation associated with antibody-based biotherapeutics.

Determine the molecular weight of low abundant species with confident with the highly sensitive DAWN MALS detector and improved peak separation with Waters BEH Column.

Explore SEC-MALS-RI multi-attribute quantification of antibodies, biosimilars, and AAVs.

Explore the optimization of AAV separation and the combined intrinsic fluorescence detection with SEC-MALS for low-concentration measurement.

In this application note, the charge variant characterisation of an IgG4-based mAb was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

GPC/SEC-viscometry enables measurement of true molar masses for carrageenan and detects subtle differences between samples with similar molar mass distributions.

GPC/SEC-MALLS analysis of hyaluronic acid obtains absolute molar mass while conventional GPC/SEC with concentration detectors only provides apparent molar masses.

Discover how SEC can be used in biopolymer applications including bioconjugates, proteins, mAbs, mRNA1, viruses, extracellular vesicles, and liposomal nanoparticles.

This paper discusses sustainable solutions for size separation of macromolecules that align with the 12 principles of green chemistry.

Tips & Tricks designed to support GPC/SEC users in their daily work, providing comprehensive overviews on different aspects of this powerful technique.

Robust, reliable GPC/SEC analysis of saccharides and polysaccharides can occur with very high resolution using Agilent SUPREMA columns with 3 and 5 µm particles.

Columns of the YMC-Pack Diol SEC series (120–300 Å), which have distinct pore sizes, were tested for their ability to separate DNA of various lengths (10–300mer).

Determine down to 8x10^9 AAV capsids and separate impurities of AAV samples by separation using a mixed-bed SEC column & highly sensitive MALS detection.

Learn the importance of light scattering detection to determine the true molecular weight distribution of a poly(glutamic acid)-based copolymer for drug-delivery.

This application note introduces application data obtained by using FlexFire SEC Columns that have adopted newly developed superior silica.

Shodex™ has successfully developed the LB-800 columns which are suitable with MALS detection. The columns are well suited to provide signal at low concentration with low molecular weight standards.

Shodex™ has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.

This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.

When selecting a suitable analytical SEC column, not only the analytical range for the fractionation, but adsorption of the target compounds, which influences the quantification and yield, should also be considered.

This application note explains how SEC coupled with MALS can be used for the determination of structural changes in polysaccharides.

This application note introduces some new application data obtained by using FlexFire SEC that is adopted by newly developed silica gel.

Field-flow fractionation coupled to multi-angle light scattering (FFF-MALS) is a powerful analytical tool that determines multiple CQAs of LNP-RNA nanoparticles.