LCGC TV: Hyphenated Techniques

HILIC was introduced more than 50 years ago but has attracted more attention recently. Giorgia Greco from the Technische Universität Munchen in Germany discusses the different options available for combining HILIC to reversed-phase LC, and how HILIC can be hyphenated with atmospheric pressure chemical ionization MS.

Performing liquid chromatography–mass spectrometry (LC–MS) analysis on tissue samples requires sample homogenization, which destroys the sample and loses its spatial resolution. Gérard Hopfgartner from the University of Geneva, University of Lausanne, Switzerland, presents an alternative method coupling liquid extraction surface analysis with modifier-assisted differential ion mobility spectrometry, describes other application areas, and gives his opinion on whether this is the beginning of the end for chromatography.

In this new video from LCGC TV, Christian Wachsmuth from the University of Regensburg in Germany, discusses how the field of metabolic fingerprinting has developed over time, and talks about why gas chromatography–mass spectrometry (GC–MS) is the ideal method for metabolic fingerprinting.

HILIC was introduced more than 50 years ago but has attracted more attention recently. Giorgia Greco from the Technische Universität Munchen in Germany discusses how the principles of HILIC differ from those of reversed-phase HPLC and how HILIC as a technique has evolved over time.

Mycotoxins are an important group of secondary metabolites produced by fungi that can cause disease in humans and animals. Active in the field of mycotoxin analysis is Rudolf Krska from BOKU, IFA-Tulln in Vienna and in this video he discusses his work investigating the human metabolism of Fusarium mycotoxins and gives his view on the future direction of mycotoxin research.

In the first video update to LCGC TV of 2015, Gérard Hopfgartner from the University of Geneva, University of Lausanne, Switzerland, discusses the advances in ion mobility mass spectrometry, highlighting the benefits of the technique and how it can be hyphenated to both 1D and 2D LC.

In this new video, Michal Holčapek from the University of Pardubice in the Czech Republic discusses the surprising advantages of LC–MALDI coupling and gives reasons why ion mobility mass spectrometry is gaining more attention.

In this new video from LCGC TV, Michal Holčapek from the University of Pardubice in the Czech Republic describes the requirements of "fast LC–MS" and areas where the technique can be applied in industry.

By 2020, more than 50% of approved drugs are expected to be protein biopharmaceuticals. Koen Sandra from the Research Institute for Chromatography in Belgium discusses the challenges of characterizing protein biopharmaceuticals and why LC–MS is now indispensable for characterizing this class of drugs.

Frederic Lynen of Ghent University in Belgium discusses why LC–MS is the ideal analytical tool for the quantitative analysis of analytes in biological samples, and presents a new HILIC-based SPE approach to improve analyte recovery and sensitivity by removing phospholipids from samples.

More and more analysts are moving away from low-resolution mass spectrometry to high-resolution mass spectrometry, but what are the requirements for making such a change? Michal Holčapek, professor of analytical chemistry at the University of Pardubice, Czech Republic, discusses what you need to consider when switching to a high resolution system. Holčapek also offers advice on how to choose the most effective ionization technique for your analysis.