Multidimensional LC

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

In the first LCGC Blog of 2025, Caitlin Cain of the University of Michigan highlights the divide between gas and liquid chromatographers, and why both types of techniques should be in the same conversations.

Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

In this installment of “LC Troubleshooting,” we describe an artifact that can arise because of compound degradation during the transfer of fractions of the first dimension (1D) column effluent to the 2D separation.

Soochow University scientists used a mass spectrometry (MS)-based system to help better understand the structure of Sulodexide.

In this month’s column, I highlight some of the primary considerations we face in method development and point to resources that can help users overcome uncertainty and develop highly effective 2D-LC methods.

During the 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology, experts presented a workshop on different ways multidimensional liquid chromatography can be applied.

Here are the top five articles that the editors of LCGC International published this week.

At Analytica 2024 in Munich, Germany, LCGC International sat down with Sebastiaan Eeltink of Vrije Universiteit Brussel to talk about three-dimensional liquid chromatography (3D–LC) separations.

Lidia Montero and Miguel Herrero from the Institute of Food Science Research in Spain discuss the evolving role of comprehensive two-dimensional liquid chromatography (LC×LC) and multi-second dimension LC×LC (multi-2D LC×LC) in food analysis.

The 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology will take place from 28–31 May 2024 in Leuven, Belgium.

This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.

The 15th Multidimensional Chromatography Workshop is a free event involving keynote and contributed presentations, a poster session, and discussion groups on all multidimensional techniques, and will be held in-person on the campus of California State University, Los Angeles, from January 10 to 12, 2024.

A comprehensive analytical approach utilizing online two-dimensional liquid chromatography coupled with hybrid linear ion trap orbital trap mass spectrometry, has enabled the in-depth analysis of Huangqi Guizhi Wuwu decoction, identifying 170 chemical constituents and offering insights into its complex chemical composition and potential therapeutic properties.

At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

Researchers from Corteva Agriscience tested a new approach for separating and quantifying small molecule structure elucidation.

The 27th International Symposium on Separation Sciences (ISSS 2023) will be held 24–27 September 2023 in Cluj-Napoca, Romania. Here’s a sneak preview of what attendees can look forwards to.

This instalment of “Column Watch” presents many of the highlighted topics and trends observed at this exceptionally well-run symposium.

Analyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.

Increases and decreases in anthocyanin concentration, which relates to the level of sugars found in red wine, were observed at specific weekly intervals of the grape ripening process.

On a fundamental level, equilibrium and diffusion define chemical separations.

High-throughput proteomic research has been hampered by the limited peak capacity of one-dimensional liquid chromatography (1D-LC) separation performance.

The two-dimensional liquid chromatography (2D-LC) separations were supplemented by the use of capillary-channeled polymer (C-CP) fiber columns.

Researchers have developed a trapping mode two-dimensional liquid chromatography (2D-LC) workflow for the enrichment and quantification of low-level impurities in pharmaceutical development, offering a promising approach for enhancing impurity monitoring in the pharmaceutical industry.

Authentication of popular ginseng-based products has been difficult and has caused confusion in quality control between traditional Chinese medicine (TCM) and Chinese patent medicine (CPM).

At HPLC 2023, LCGC spoke with Andrea Gargano of the Van 't Hoff Institute for Molecular Science at the University of Amsterdam in The Netherlands. These clips comprise one of several HPLC interviews that were conducted at the conference.

At HPLC 2023, Paola Dugo held a lecture on how combining two-dimensional liquid chromatography (LC×LC) with mass spectrometry (MS) can help scientists analyze food samples.

Talks by Dwight R. Stoll, Tadeusz Górecki, André de Villiers, and Andrea Gargano highlighted the after-lunch presentations at HPLC 2023 on June 20.