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Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
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The Impact of Column Hardware on Efficiency in Liquid Chromatography (LC)Column hardware in liquid chromatography—that is, the tubing, the frits, and fitting—has a strong influence on separation performance or the reproducibility of chromatographic data. Flow distributors and frits can introduce serious band broadening when separation is performed in short, narrow-bore columns. The kinetic performances of packed and monolithic columns are usually similar for well-retained analytes. However, monolithic columns may show significantly better performance for early-eluting compounds, which can be attributed to the moderate mobile phase dispersion caused by the simpler column hardware because no frits are required.
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Unique high-throughput ENRICH-iST workflow for deeper plasma proteome coverage enables discovery of novel biomarkersThis high-throughput proteome profiling approach provides deeper proteome coverage with preserved biological information. The ENRICH-iST technology enables the analysis of previously undetected low- abundance proteins, facilitating the discovery of additional potential biomarkers.
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Validation of HPLC-DAD Based Stability Indicating Assay Method for Ornidazole in Periodontal Polymeric Hydrogel: Robustness Study using Quality by Design (QbD) ApproachIn the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.
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A Method Transfer from HPLC to UHPLC with Example of PolyphenolsWe recently introduced a new series of UHPLC columns with a particle size of 1.6 µm. Here we explain how to transfer an existing HPLC method to a new UHPLC method using polyphenols as an example.
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Ensuring Data Integrity in the CloudThere’s a lot of interest in moving data to the cloud for security, centralization, and collaboration. Doing so, however, has implications for data integrity and audits. To understand more, we spoke to Monica Cahilly, the president and founder of Green Mountain Quality Assurance, in a podcast interview. Cahilly has more than 25 years of consulting experience, with specialized interest and enthusiasm for data integrity assurance and data governance. A short excerpt of that interview is presented here.
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Take Control of ResolutionSeparation scientists may seek an optimum spot between chromatographic performance required to obtain sufficient results quality, and the time and resources needed to do so. This instalment of “GC Connections” examines the factors that control peak resolution-one of the main drivers of separation quality-and how chromatographers can use this information to find an optimum between time, cost, and performance.
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Innovation in Nutrition and Food Analysis: The FoodOmicsGR_Research InfrastructureFoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
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Analysis of Organophosphorus Pesticides by HPLC Using Magnetic SPE with Nitrogen-Doped Reduced Graphene Oxide/Fe3O4 Nanocomposite as the AdsorbentIn this study, a method was developed for extraction and preconcentration of trace amounts of organophosphorus pesticides (OPPs), including fenitrothion, chlorpyrifos-methyl, and chlorpyrifos in environmental water.
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Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LCAnalyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
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On-Column Digest Followed by SPE DesaltingTecan’s proprietary NBE™ format enables digestion or incubation AND elution in a single SPE column. Read this app note to learn more and streamline your workflows!
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Affinity Selection-Mass Spectrometry: Defining the Bioactive Compounds in Complex Mixtures of Natural Products and Combinatorial LibrariesDrug discovery using high-throughput screening of discreet compounds, and the discovery of natural products with pharmacological mechanisms of action, rely on bioassay-guided fractionation analysis. Recent applications of affinity selection–mass spectrometry (AS-MS) are useful for exploring the discovery of ligands to membrane-bound proteins and RNA targets.
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Top-Down Mass Spectrometry of Intact Proteins and Complexes: From Rare to RoutineIn recent years, top-down mass spectrometry has quickly advanced from academic rarity to (near-) routine. How did we get here? And what can we expect in the near future?
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Development of a “Freeze-Pour” Sample Preparation Method for the GC Analysis of Semivolatile Flavouring Chemicals Present in E-cigarette Refill LiquidsHS-GC analysis is presented as an excellent method for the analysis of high volatile components in e-liquids. For the analysis of semivolatile ingredients, an additional sample preparation step is proposed based on a LLE followed by a freeze-out of the matrix components.
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Development of a Standardized Protocol for the Classification of Column Sets in Comprehensive Two-Dimensional Gas ChromatographyThe article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
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Methods for the Passivation of HPLC Instruments and ColumnsNonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.
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In-Loop Analyte Degradation in Two-Dimensional Liquid Chromatography: Example and SolutionsIn this installment of “LC Troubleshooting,” we describe an artifact that can arise because of compound degradation during the transfer of fractions of the first dimension (1D) column effluent to the 2D separation.
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The Effects of Inner Surface Roughness of Microbore Column Blanks on Column PerformanceThe efficiencies of microbore-2 columns, which are prepared from blanks that have a wide variety of inner surface roughness, drop sharply when the size of individual surface roughness features approaches the particle size of the packing material. The results suggest that two categories of packed column structure relate to the surface features and yield high and low efficiency columns. This installment of "Column Watch" discusses this conclusion in terms of the stability of an agglomerated layer of packing particles on the blank wall when subjected to shear forces during column packing.
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Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
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Optimal Separation of Polar Anionic Pesticides from Fruits and Vegetables with HPLC Column SelectivityThis article presents a new column technology that uses reversed phase separation and offers excellent peak shape, resolution, and robustness for a wide variety of food matrices.
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Online Solid-Phase Extraction–Gas Chromatography–Flame Ionization Detection System for Monitoring Contaminants at Parts-Per-Trillion Concentrations in Process WatersOnline monitoring of odour and taste components that occur at parts-per-trillion (ppt) levels in industrial process waters requires specialized analytical hardware that is generally not compatible with the harsh environmental conditions in these typical industrial settings. An alternative instrumental method is proposed that uses dynamic extraction in combination with gas chromatography (GC) equipped with a simple flame ionization detector (FID) to achieve these extremely low detection limits.
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A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in NeonatesThis simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
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Cannabis Metabolomic Data Processing: Challenges to be AddressedThe challenges that arise during cannabis metabolomics analysis using ultrahigh-performance reversed-phase liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC–reversed-phase–HRMS/MS) are presented.
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Identification of Microplastics in Environmental Monitoring Using Pyrolysis–GC–MS AnalysisPyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Determination of Polychlorinated Biphenyls in Water Samples Using a Needle Trap Device Combined with Gas ChromatographyThe method presented here, which uses a novel needle trap device (NTD) with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.
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Electromembrane Extraction with Vials of Conducting PolymerIn electromembrane extraction (EME), the target analyte is extracted from an aqueous sample across a supported liquid membrane (SLM) and analyzed using LC. Through the study presented here, we demonstrate the principles of the technique and test its performance.
