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The Role of Ion Pairing Agents in Liquid Chromatography (LC) SeparationsWhen used appropriately in RPLC, ion pairing agents can increase the separation and retention of charged analytes. But should they be explored in other modes of HPLC as well?
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Development of Highly Sensitive LC–MS and CE–MS Methods for In-Depth Proteomic and Glycomic Profiling of Limited Biological SamplesBetter sample preparation and miniaturized separations are enhancing these analyses.
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A Standardized 2D-LC Screening Platform for Peak Purity Determination in Pharmaceutical AnalysisThis study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.
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5 Things to Consider When Selecting a Chromatography Silica (Sept 2024)Particularly in the pharmaceutical industry, drug purity isn’t just a goal – it’s essential for achieving safety, stability and efficacy. However, purification is easier said than done, especially with challenging molecules like DNA and RNA “oligonucleotides,” due in large part to their diversity and the range of impurities that can be generated during production. Enter DAVISIL® chromatographic silica, with a wide range of pore diameters and particle sizes to meet your specific application, performance and sustainability requirements. Before you choose the chromatography resin for your next purification application, take a look at these 5 considerations.
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Supercritical Fluid Chromatography for Chiral Analysis, Part 2: ApplicationsIn the second part of this review article, the recent progress in SFC for enantiomeric separations is evaluated. Several applications reported on the enantioselective separation of drugs and pharmaceutical compounds using chiral SFC are discussed, including pharmaceutical applications, clinical research, forensic toxicology, and environmental sciences.
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mRNA Characterization, from 5’ Cap to Poly (A): What Ion-Pair Reversed-Phase Liquid Chromatography (IP-RPLC) Analysis Can Tell YouThis article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.
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Analysis of PFAS in Locally Acquired Food ContainersThe combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.
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Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
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Benefits of Integrating Travelling Wave Ion Mobility Spectrometry into Liquid Chromatography and Mass Spectrometry Workflows for Steroid AnalysisThe advantages provided by the implementation of ion mobility spectrometry (IMS), and in particular travelling wave ion mobility spectrometry (TWIMS), in traditional liquid chromatography–mass spectrometry (LC–MS) systems are discussed.
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Challenges in the Analytical Characterization of VLPs Through HPLC-Based MethodsThis article discusses the challenges and effective solutions for high performance liquid chromatography (HPLC)-based analytical characterization of virus-like particles (VLPs).
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Superheated Water: A New Look at Chromatographic Eluents for Reversed-Phase HPLCThe authors describe the results they've achieved by using water heated to 100–240 ºC as a liquid eluent for reversed-phase HPLC instead of an organic modifier. They point out that this alternative avoids many of the problems – toxicity, flammability, and cost – associated with organic modifiers.
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Tackling PFAS Complexity with HRMS and Bioanalytical TechniquesThere are many benefits of using high-resolution mass spectrometry (HRMS) in analyzing PFAS accumulating in living organisms. This article shows how HRMS, when combined with exposure-relevant mixtures, can help elucidate more about PFAS toxicity and exposure.
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Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
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The Impact of Column Hardware on Efficiency in Liquid Chromatography (LC)Column hardware in liquid chromatography—that is, the tubing, the frits, and fitting—has a strong influence on separation performance or the reproducibility of chromatographic data. Flow distributors and frits can introduce serious band broadening when separation is performed in short, narrow-bore columns. The kinetic performances of packed and monolithic columns are usually similar for well-retained analytes. However, monolithic columns may show significantly better performance for early-eluting compounds, which can be attributed to the moderate mobile phase dispersion caused by the simpler column hardware because no frits are required.
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Unique high-throughput ENRICH-iST workflow for deeper plasma proteome coverage enables discovery of novel biomarkersThis high-throughput proteome profiling approach provides deeper proteome coverage with preserved biological information. The ENRICH-iST technology enables the analysis of previously undetected low- abundance proteins, facilitating the discovery of additional potential biomarkers.
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Validation of HPLC-DAD Based Stability Indicating Assay Method for Ornidazole in Periodontal Polymeric Hydrogel: Robustness Study using Quality by Design (QbD) ApproachIn the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.
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A Method Transfer from HPLC to UHPLC with Example of PolyphenolsWe recently introduced a new series of UHPLC columns with a particle size of 1.6 µm. Here we explain how to transfer an existing HPLC method to a new UHPLC method using polyphenols as an example.
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Retaining Talent in Field-Flow Fractionation: An InitiativeThe authors present their motivation for establishing the Young Scientists of FFF (YSFFF) initiative within the FFF community.
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Ensuring Data Integrity in the CloudThere’s a lot of interest in moving data to the cloud for security, centralization, and collaboration. Doing so, however, has implications for data integrity and audits. To understand more, we spoke to Monica Cahilly, the president and founder of Green Mountain Quality Assurance, in a podcast interview. Cahilly has more than 25 years of consulting experience, with specialized interest and enthusiasm for data integrity assurance and data governance. A short excerpt of that interview is presented here.
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Take Control of ResolutionSeparation scientists may seek an optimum spot between chromatographic performance required to obtain sufficient results quality, and the time and resources needed to do so. This instalment of “GC Connections” examines the factors that control peak resolution-one of the main drivers of separation quality-and how chromatographers can use this information to find an optimum between time, cost, and performance.
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Innovation in Nutrition and Food Analysis: The FoodOmicsGR_Research InfrastructureFoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
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Analysis of Organophosphorus Pesticides by HPLC Using Magnetic SPE with Nitrogen-Doped Reduced Graphene Oxide/Fe3O4 Nanocomposite as the AdsorbentIn this study, a method was developed for extraction and preconcentration of trace amounts of organophosphorus pesticides (OPPs), including fenitrothion, chlorpyrifos-methyl, and chlorpyrifos in environmental water.
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Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LCAnalyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
