Application Notes: LC-MS

A comparison made between a stainless steel hardware column and an INERT column using the same lot of HALO 90 Å C18, 2.7µm results in tailing factor and area improvement using the HALO®INERT column.

Using HALO®Elevate C18 and a high pH mobile phase obtains a great separation of drugs of abuse and metabolites without the use of a specialized stationary phase.

The positively charged surface chemistry of HALO® PCS Peptide delivers narrower peak width, higher signal response and double the peak capacities compared to standard uncharged peptide materials

Over the course of 5000 injections, the HALO®Elevate C18 1.5 mm ID column demonstrates stability and robustness in the stationary phase and the column hardware, with no significant changes to the retention time, peak shape, and back pressure.

A mix of four antidepressants is separated using the HALO®PCS (positive charged surface) C18 column demonstating the improved tailing factor and efficiency when compared to a traditional (uncharged) C18 stationary phase.

The HALO® OLIGO C18 2.7 µm column outperformed an FPP Oligo C18 column in oligonucleotide separations, achieving faster separations while maintaining efficiencies comparable to a 1.9 µm FPP with more than half the backpressure.

A separation of ultra-short and long chain PFAS (C1-C18) is performed on a HALO®PCS Phenyl-Hexyl column along with a HALO®PFAS Delay column which demonstrates excellent retention for both hydrophilic and hydrophobic analytes.

Crucial phosphorylated compounds can be analyzed by a polymeric iHILIC-(P) Classic HILIC column and MS. The method is applicable for targeted and non-targeted metabolomics.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

In this application note, the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

The non-targeted screening of polar metabolites in human plasma with high sensitivity is achieved using a bioinert YMC Accura Triart Diol HILIC column.

The analysis of human plasma samples using a bioinert coated YMC Accura Triart C8 column demonstrates that real-life lipidomic analysis in under 10 minutes is possible.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

This application note demonstrates the analysis of 9 halogenated acetic acids, which are regulated by the EPA and the European Union, using ICPMS/MS for detection.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

HPLC coupled with negative ESI-MS provides a reliable method with unique capabilities for separation and identification of (therapeutic) oligonucleotides.

Microplastics in water are a major concern, found even in tap water and human bodies. Learn how Sartorius Arium® Pro VF series removes them effectively.

In this poster, we explore parameters that could impact data integrity with the analytes in EPA Method 1633, UCMR 5 and the EU Drinking Water Directive regulations.

Measuring ultrashort-chain PFAS (C1-C4) in water is crucial due to their ubiquity and potential human exposure. Standard RPLC tests struggle with these compounds.

Achieve exceptional peak shapes in LC-MS analysis of the challenging phospholipids LPC and PC with the bioinert coated YMC Accura Triart C18 columns.

Explore the game-changing benefits of the bioinert YMC Accura Triart C18 columns for phosphorylated peptide analysis to achieve reproducible results.

The non-targeted screening of polar metabolites in human plasma with high sensitivity is achieved using a bioinert YMC Accura Triart Diol HILIC column.

The analysis of human plasma samples using a bioinert coated YMC Accura Triart C8 column demonstrates that real-life lipidomic analysis in under 10 minutes is possible.

The analysis of 3 different RNA and DNA oligonucleotide mixtures demonstrates the supremacy of the bioinert coated YMC Accura Triart Diol-HILIC columns.

Measuring ultrashort-chain PFAS (C1-C4) in water is crucial due to their ubiquity and potential human exposure. Standard RPLC tests struggle with these compounds.

Crucial phosphorylated compounds can be analyzed by a polymeric iHILIC-(P) Classic HILIC column and MS. The method is applicable for targeted and non-targeted metabolomics.

In this short note, we will consider the selection of LC procedures to separate diniconazol enantiomers and show how data automation improves the process.

This application note discusses how to streamline method transfer outcomes using ACD/Labs' method development software, Method Selection Suite.

This application note demonstrates a novel composite technology that enhances reproducibility of solid phase extraction compared to a traditional loose packed format.