Timo F. Beskers previews his tutorial lecture at HPLC 2023 in Düsseldorf, where he will give an overview on polymer REACH, its expected regulatory requirements, challenges, and analytical needs, as well as strategies to deal with those from the perspective of polymer chromatography.
A generalized approach using DoE is presented for the optimization of DHS extraction parameters.
Reversed-phase LC–MS has limitations in numerous analytical applications. Alternative MS-compatible chromatographic techniques separate analytes in the liquid phase based on different retention mechanisms compared with reversed-phase LC. This article describes these alternative chromatographic approaches, relevant applications, and the future of these techniques.
Headspace-solid-phase microextraction (HS-SPME) is widely used for the analysis of volatile compounds from different kinds of samples.
This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.
An examination of how greening analytical methods is directly connected to greening the sample preparation step
Non-proximate MS presents challenges for ion transmission and preventing loss of analyte signal. For continuous, direct MS analysis, there are four models for non-proximate MS that work best.
Acoustic ejection mass spectrometry (AEMS) has recently emerged as the premier ultrahigh-throughput mass spectrometric methodology for drug discovery and related fields.
The success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
The basic operating principles of ion mobility spectrometers (IMS), their resulting strengths and weaknesses, and why both perfectly align with the capabilities and requirements of gas chromatographs are discussed.
This review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
Pyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
This information is supplementary to the article “Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates” that was published in the March 2022 issue of LCGC Asia Pacific.
Although well known, the extent of adduct ion formation in LC–ESI-MS/MS, and the implications for quantitative analysis and analyte characterization are not fully appreciated. We explore this problem and explain the implications for reproducibility, quantitative and qualitative analyses, and DDA and DIA.
This month's "CE Currents" continues an examination of problems that users may find in routine CE work. Readers can avoid these problems by adopting the working practices suggested by the authors.
The purpose of this short tutorial article is to review the terms and official nomenclatures for size-exclusion separations and to provide some guidance and recommendations for practicing chromatographers. The interconversion between the different metrics is explained and some examples are presented.
In this study, the experimental nontargeted screening approach and corresponding data analysis workflows—simultaneously using molecular ion information and structural information—are presented for the molecular identification and authenticity verification process from a brand perfume using GC–ecTOF-MS.
A look at the LC and LC–MS methods that are helping to overcome challenges in sample analysis, and how this can make adeno‑associated virus (AAV)–based gene therapy more accessible.
A HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.
The authors describe the results they've achieved by using water heated to 100–240 ºC as a liquid eluent for reversed-phase HPLC instead of an organic modifier. They point out that this alternative avoids many of the problems – toxicity, flammability, and cost – associated with organic modifiers.
This month's "Column Watch" describes moving bed chromatography and how the concept is realized in the form of simulated moving bed chromatography. Also included is an inside look at instrumentation, method development, and applications for industrial purifications.
The study of the thermal behaviour and pyrolysis products of these terpenoids could possibly suggest flavour precursors that could be used to provide specific flavours to tobacco.
Gas chromatography–mass spectrometry (GC–MS) with cold electron ionization (EI) is based on interfacing the GC and MS instruments with supersonic molecular beams (SMB) along with electron ionization of vibrationally cold sample compounds in SMB in a fly-through ion source (hence the name cold EI). GC–MS with cold EI improves all the central performance aspects of GC–MS. These aspects include enhanced molecular ions, improved sample identification, an extended range of compounds amenable for analysis, uniform response to all analytes, faster analysis, greater selectivity, and lower detection limits. In GC–MS with cold EI, the GC elution temperatures can be significantly lowered by reducing the column length and increasing the carrier gas flow rate. Furthermore, the injector temperature can be reduced using a high column flow rate, and sample degradation at the cold EI fly-through ion source is eliminated. Thus, a greater range of thermally labile and low volatility compounds can be analyzed. The extension of the range of compounds and applications amenable for analysis is the most important benefit of cold EI that bridges the gap with LC–MS. Several examples of GC–MS with cold EI applications are discussed including cannabinoids analysis, synthetic organic compounds analysis, and lipids in blood analysis for medical diagnostics.
Two alternative approaches based on gas chromatography coupled to tandem mass spectrometry (GC–MS/MS) were developed to control the maximum residue limit of the banned insecticide, ethylene oxide (EtO), and its transformation product, 2-chloroethanol (2-CE).
How can low-resolution mass spectrometry (LRMS) and high-resolution (HR) MS work together to provide unambiguous results in clinical and forensic toxicology?
This application note will illustrate how a new integrated pump controller is giving users automated control of pump operations in critical biopharma applications.
How to speed up sample prep time and equivalent acccuracy and precision.
In this instalment of “Column Watch”, a simple method for profiling free naphthenic acids in produced water by GC–MS is proposed.