Author


Jelena Petrovic

Latest:

Analysis of Boronic Compounds as Potential Mutagenic Impurities in Drug Substances By GC–MS

This novel gas chromatography (GC)–MS approach enables analysis of the suspected mutagens bis(pinacolato)diboron (BPD) and tetrahydroxydiboron (BBA) found at low-levels in certain intermediates and active pharmaceutical ingredients (APIs), even in the presence of boron from other sources.


Debby Bracke

Latest:

Detailed Glycosylation Analysis of Therapeutic Enzymes Using Comprehensive 2D-LC–MS

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.


Silvia Millán-Martín

Latest:

Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.


Y. Hua

Latest:

In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes Analysis

A new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.


Sylvain Lehmann

Latest:

LC–MS/MS for the Quantification of Tau and Phosphorylated Tau in Alzheimer’s Disease Patients’ CSF

Quantification of Tau proteoforms in cerebrospinal fluid (CSF) could be useful to diagnose neurodegenerative diseases. LC–MS/MS results can help explain the underlying molecular mechanisms of disease evolution.


Zhonghua Shen

Latest:

A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS Transmission

The investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.


Orapin Chienthavorn

Latest:

Superheated Water: A New Look at Chromatographic Eluents for Reversed-Phase HPLC

The authors describe the results they've achieved by using water heated to 100–240 ºC as a liquid eluent for reversed-phase HPLC instead of an organic modifier. They point out that this alternative avoids many of the problems – toxicity, flammability, and cost – associated with organic modifiers.


The Chromatography Forum of the Delaware Valley

Latest:

Dal Nogare Session Highlights Approaches for Difficult Separations, Improved Efficiency, Better Sustainability, and Career Success

Because the Covid-19 pandemic of 2020–2023 disrupted yearly face-to-face events, the Dal Nogare Award Symposium at Pittcon 2023 included both the 2021 and 2022 winners. Read more about the symposium here.



Selina Tisler

Latest:

Separation Science: The State of the Art: Nontargeted Screening in Environmental Analysis: An Example Showing Migration of Chemicals from Reusable Sports Plastic Bottles into Drinking Water

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.


Antonio Ricci

Latest:

The Role of Adsorption and pH of the Mobile Phase on the Chromatographic Behavior of a Therapeutic Peptide

We explore the impact of two different stationary phases and ion-pair reagents on the retention behavior of a therapeutic peptide using reversed-phase liquid chromatography. This information is of fundamental importance for the development of reliable, selective, and fast analytical methods able to separate and identify the target peptide.


Rahmat Ullah

Latest:

New Technologies That Accelerate Persistent Organic Pollutant (POP) Sample Preparation

Accelerated solvent extraction (ASE) is widely viewed as a better alternative to GC–MS when analyzing persistent organic pollutants (POPs). The authors explain why new technological developments offer many benefits and accelerate POP sample preparation processes.


Michael J. Feeney

Latest:

Achieving Optimum GC Separation – Designing New Capillary Column Stationary Phases Using Computer Modeling

By calculating optimal stationary-phase composition and physical parameters, column developers can construct columns that provide the exact separation requirements desired by analysis or current methods.


Zongyun Huang

Latest:

Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry Perspective

This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.


Gopal Prasad Agrawal

Latest:

Validation of HPLC-DAD Based Stability Indicating Assay Method for Ornidazole in Periodontal Polymeric Hydrogel: Robustness Study using Quality by Design (QbD) Approach

In the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.


Trevor I. Smith

Latest:

LCGC and ACS Subdivision on Chromatography and Separations Chemistry Survey 2023: What Skills Do New Analytical Chemistry Employees Need?

For this year’s career issue, LCGC North America teamed up with the American Chemical Society Subdivision on Chromatography and Separations Chemistry to ask the analytical chemistry community what skills new employees in the field need to succeed. In this report, we analyze the survey results and explore how they can inform the future of analytical chemistry curriculum development.


Xiaohui Zhang

Latest:

Using the Empore EZ-Trace SPE Workstation to Perform Efficient and Reproducible Extractions of SVOCs

This application note demonstrates how the Empore EZ-Trace SPE system is used to perform multiple extractions simultaneously while achieving clean and reproducible results using EPA Method 525.3 as an example application.


Ivan Lebedev

Latest:

Method Robustness and Reproducibility for Monoclonal Antibody Purity Analysis

The benefits of a robustness assessment for the analysis of a NIST mAb using a wide pore C4 LC column are described.


Andrew Stark

Latest:

Supplementary Info: Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates

This information is supplementary to the article “Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates” that was published in the March 2022 issue of LCGC Asia Pacific.


Claire Dauly

Latest:

Separation of Isomeric Metabolites and Gangliosides with High Performance (Drift Tube) Ion Mobility–Mass Spectrometry

Employing ion mobility at atmospheric pressure without vacuum enables coupling to high-resolution mass analyzers. High performance ion mobility–orbital trap mass spectrometry (HPIM-OT-MS) is a useful alternative for separating isomers, such as isomeric metabolites seen in drug discovery.




Christina Virgiliou

Latest:

Innovation in Nutrition and Food Analysis: The FoodOmicsGR_Research Infrastructure

FoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.



Line Noreng

Latest:

On the Surprising Retention Order of Ketamine Analogs Using a Biphenyl Stationary Phase

An unexpected retention order for ketamine analogs was observed when using a biphenyl stationary phase for liquid chromatography-mass spectrometry (LC–MS).


Ron Bonner

Latest:

The Origin and Implications of Artifact Ions in Bioanalytical LC–MS

Although well known, the extent of adduct ion formation in LC–ESI-MS/MS, and the implications for quantitative analysis and analyte characterization are not fully appreciated. We explore this problem and explain the implications for reproducibility, quantitative and qualitative analyses, and DDA and DIA.


Juby Mathew

Latest:

Rapid Quantitative Analysis of Ethylene Oxide and 1,4-Dioxane in Polymeric Excipients Using SIFT-MS

Pharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>.


Florian Füssl

Latest:

Exploring Capillary Electrophoresis–Mass Spectrometry for the In-Depth Intact Native Analysis of Cysteine-Conjugated Antibody-Drug Conjugates

This article explores the potential of microchip capillary electrophoresis–mass spectrometry (CE–MS) for the characterization of intact biopharmaceuticals, and how this technique can effectively be employed to monitor essential quality attributes of interest.


Michael Hicks

Latest:

Book Review: Practical Application of Supercritical Fluid Chromatography for Pharmaceutical Research and Development

Why is SFC becoming increasingly more important as a sustainable analytical technique?


Fred Klink

Latest:

New Sample Preparation Approaches to Biological Matrices for LC–MS

Current sample preparation procedures for LC–MS as applied to samples from biological matrices.