Analytical Instrumentation

In this column, we look at the current version and the update of USP <621> on high-performance liquid chromatography (HPLC) that becomes effective 1st May 2025.

At HTC-18 in Leuven, Executive Editor of LCGC International, Alasdair Matheson, spoke to Brett Paull from the University of Tasmania and HyTECH about his talk entitled: “Analytical Performance and Disruptive Potential of Fully Portable Portable and Field Deployable LC–MS Technology.”

Are you a chromatographer or a chromatography user?

In this edition of "The LCGC Blog," Jonathan Shackman discusses the differences between chromatographers and chromatography users, as well as advancements that have made chromatography a more accessible field.

A group of Malaysian and American scientists recently analyzed the benefits of using green metrics for detecting emerging contaminants (ECs) using analytical methods.

Splitless modes of operation using an optimized pulse injection are described.

A collaborative multi-analyte method developed utilizes HPLC and UHPLC to analyze more than 70 active ingredients. This method offers a validated approach for determining technical AI alongside linearity, precision, accuracy, and specificity tests for seven active ingredients.

Mary Ellen McNally and Doug Raynie covered green chemistry and sustainable separations, from sample preparation through analysis with chromatographic techniques, at EAS 2023.

The impact of contamination on each part of the inlet is examined.

The author discusses the benefits and challenges of using hydrogen in the laboratory, specifically regarding gas chromatography and GC–MS procedures.

Studies published over the last five years have focused on fully or semi-automated approaches and their applications in extraction of both organic and inorganic pollutants, while modern robotic tools and 3D printing are starting to make impacts.

This instalment explores the challenges involved in detecting the effluent from a capillary gas chromatographic column and summarizes the many detector options.

Researchers have introduced MCnebula, a new framework for untargeted LC–MS/MS data analysis. MCnebula focuses on critical chemical classes and utilizes visualization techniques to streamline the analysis process, enabling efficient pathway analysis, biomarker discovery, and classification of unknown compounds beyond the limits of spectral libraries.

A new way to control ion transports in microfluidic systems has the potential to improve the technology and expand applications.

With Shimadzu Corporation's new location, the company hopes to innovate their research and technological efforts.

This comparison of various silica-based HILIC stationary phases with different types of coatings provides a better understanding of how stationary phase selectivity can be influenced.

When the unique shape-selective properties of PGC are appropriately identified, PGC stationary phases can be harnessed for fine separations of structurally related compounds.

An examination of the new high performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products that were introduced over the last year.

Given that artificial intelligence (AI) seems to be on everyone’s mind, it’s worth pausing to look at how AI works, and how it can fit into analytical chemistry.

This installment of Tips & Tricks will provide some helpful pointers to keep your GPC/SEC system in good shape.

With suspect PFAS screening becoming more popular, and the list of known PFAS now in the thousands, novel analytical tools are needed to generate transferable data sets. We highlight key updates here.

Online 2D-LC is a powerful—and accessible—tool for analyzing pharmaceutical and biopharmaceutical samples, in both R&D and QC. It’s now easier than ever to develop 2D-LC methods, and they can be run in a fully automated manner.

Is the informing power of separation science without limits?

The fundamental mechanisms of band broadening are usually introduced to students through the van Deemter equation. Dimensional analysis of this equation can give physical meaning to the equation coefficients and enhance our understanding relative to qualitative descriptions. This approach can also guide improvements to future liquid chromatography (LC) column designs.

Further miniaturization of separations will greatly extend the reach of single-cell proteomics, metabolomics, and lipidomics, but key challenges in instrumentation, column technology, and ionization sources must be addressed.

This paper provides an overview of some current and emerging physicochemical analytical challenges associated with these sophisticated drug systems and some of the technical advances needed in chromatographic systems to enable their design, development, and manufacture.

As two-dimensional liquid chromatography (2D-LC) becomes more widely used, system suitability tests (SSTs) become even more important.

With a new prototype, we explore how to improve the resolution power and usability of LC–MS instruments for routine analysis in pharmaceutical applications.

New sample preparation workflows for intact proteins in biological matrices are needed. We hope that others will join in this important field of research.