Liquid Chromatography (LC/HPLC)

In this installment of "LC Troubleshooting," Dwight Stoll discusses several essential principles related to when and why buffers are important, as well as practical factors, such as commonly used buffering agents, that are recommended for use with different types of detectors.

The Chromatographic Society (ChromSoc) has announced the winners of the Martin Medal and the Silver Jubilee Medal for 2025. Professor Bogusław Buszewski of Nicolaus Copernicus University in Torun, Poland, has been awarded the prestigious Martin Medal, and the 2025 Silver Jubilee Medal has been awarded to Elia Psillakis of the Technical University of Crete in Greece.

Known as “forever chemicals”, PFAS exposure has been linked indirectly and directly to a range of potential health issues. The objective of this study was the development of an optimized sample preparation and LC–MS/MS method for the analysis of PFAS in serum for biomonitoring applications.

Recently, we talked with Jim Grinias of Rowan University about his experiences at EAS 2024 and his expectations for analytical chemistry trends in 2025.

Shanghai University of Sport scientists recently developed a doping control method based on gas chromatography combustion isotope ratio mass spectrometry (GC/C/IRMS).

As part of our RAFA 2024 coverage, we interviewed Rudolf Krska of BOKU University about his research on food safety, specifically regarding using analytical methods to mycotoxins and other secondary metabolites in plants and foods.

Giorgia Purcaro from the University of Liège was interviewed at RAFA 2024 by LCGC International on the benefits of modern multidimensional GC methods to analyze mineral oil aromatic hydrocarbons (MOAH) and mineral oil saturated hydrocarbons (MOSH).

Michael Sulyok of the University of Natural Resources and Applied Life Sciences discusses a novel “dilute and shoot” LC–MS/MS technique to analyze mycotoxins in grain products.

Here is some of the most popular content posted on LCGC International this week.

A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).

Here is some of the most popular content posted on LCGC International this week.

The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, June 15-19, 2025, in Bruges, Belgium.

LCGC International spoke with various corporate personalities about trends and developments in sample preparation.

LCGC International interviewed FeMS Empowerment Award winner Marta Relvas-Santos on her use of mass spectrometry to identify potential biomarkers and therapies for bladder cancer. She also shared insights on her work with FeMS and advice for fellow scientists.

This article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.

Saer Samanipour from the Van ‘t Hoff Institute for Molecular Sciences (HIMS) at the University of Amsterdam spoke to LCGC International about the benefits of a data-driven reversed-phase liquid chromatography (RPLC) approach his team developed.

Here is some of the most popular content posted on LCGC International this week.

Mithibai College of Arts scientists recently used liquid chromatography-tandem mass spectrometry (LC–MS/MS) for detecting nitrosamines in blood pressure medication.

Informative panel discussions featuring key opinion leaders from the separation science sector discussing the latest technology trends and developments in liquid chromatography, gas chromatography, sample preparation, and data handling.

Scientists recently developed new GC/LC-Q-TOF/MS-based method for determining pesticides in Angelica sinensis, a traditional Chinese medicine.

LCGC International sat down with Faith Johnson of ECOLAB to discuss her career and work with the Females in Mass Spectrometry (FeMS) group.

A study has used gas chromatography coupled to a time-of-flight mass spectrometer to build an electron ionization mass spectra database of more than 250 chemicals classified as either volatile or semi-volatile compounds. An additional, confirmatory layer of liquid chromatography–mass spectrometry analysis was subsequently performed.

The evolution of LC–MS for characterizing mRNA therapeutics is discussed.

Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?

Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.

Here is some of the most popular content posted on LCGC International this week.

Biotech Fluidics recently released the Biotech Micro Flow Meter, a tool for continuously monitoring the consistency of nanoscale dispensing systems as well as UHPLC and LC/MS solvent/reagent delivery systems.

Biotech Fluidics reports that Dr Maisch HPLC GmbH have installed a Biotech Liquid Micro Flow Meter in their testing set-up to validate the performance of all capillary columns it manufactures.

Scientists from the Institute of Analytical Chemistry of the Czech Academy of Sciences and Masaryk University recently tested various saliva sampling methods to optimize bile acid analysis.

Michael G. Bartlett of the University of Georgia looked at how hydrophilic interaction liquid chromatography (HILIC) has evolved for oligonucleotide analysis in a new article.