Tips & Tricks

Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.

This instalment of Tips & Tricks discusses the effect of column order on separation efficiency.

The benefits of a molar mass distribution over molar mass averages and the differences between the different representations of molar mass distributions are discussed.

Did you know that applying the second derivative as a pretreatment to your near-infrared spectral data can help resolve overlapped peaks?

A key advantage of core-shell columns is that their performance can rival that of the traditional fully porous particle columns, but at somewhat larger particle sizes and at significantly lower backpressures.

Thermal desorption (TD) is a versatile preconcentration technique for gas chromatography (GC) that is used to analyze volatile and semi-volatile organic compounds in air, materials or liquids.

Combined with chromatography media, 96-well filter plates have emerged as an efficient tool for the fractionation of small-volume protein samples.

Extra column volume is the volume in an HPLC system external to the column that contributes to the total peak volume, which will impact peak width, resolution, and overall efficiency.

A main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.

With the introduction of the timsTOF platform, the next evolution in technology is achieved by adding an additional dimension of gas phase ion separation via the use of TIMS coupled to UHR-QTOF technology.