LCGC TV: Liquid Chromatography

The Kinetic Plot method is a useful tool in LC method development to compare the performance of different LC columns with each other. In this new video from LCGC TV, Deirdre Cabooter from KU Leuven (Belgium) describes how to use the variable length method development strategy to develop successful UHPLC methods and how this approach can save time for the analyst.

In these two new videos from LCGC TV, Mary Ellen McNally from DuPont Crop Protection addresses the issue of precision, covering the reasons why the precision of a measurement is so important and the factors that can influence the precision of an active ingredient assay.

Ultrahigh-performance liquid chromatography (UHPLC) offers improved run-times and enhanced resolution when compared with conventional liquid chromatography (LC) methods. In this new video from LCGC TV, Richard Henry, a consultant from State College (Pennsylvania, USA), gives his advice on how to select UHPLC column size and discusses the features of an optimal UHPLC instrument.

Do you want to get more out of modern high performance liquid chromatography (HPLC) systems? In this new video from LCGC TV, Richard Henry, a consultant from State College (Pennsylvania, USA), discusses what chromatographers need to learn, from measuring dispersion and using Van Deemter and Van ‘t Hoff plots to getting familiar with mobile phase variables.

Fully porous particle columns with narrow particle distribution can improve the efficiency of HPLC separations. In this new video from LCGC TV, Richard Henry, a consultant from State College (Pennsylvania, USA) discusses the advantages of narrow particle distribution and whether optimized LC instruments are required for optimum performance.

Transferring methods between laboratories can be extremely difficult. In this new short video from LCGC TV, Mary Ellen McNally from DuPont Crop Protection gives her advice on best practices for maintaining precision during method transfer.

We all make mistakes. The first step to correcting them is recognizing them, and learning the right way to do things. In two new short videos, Merlin Bicking talks about two common errors that chromatographers make, and how to avoid them.

We all have always been told that we need to listen, and to listen more carefully. Growing up, we were told to listen to our parents and our teachers. As adults, we need to listen more attentively to our spouses at home and our bosses, colleagues, and customers at work. But have you considered listening to your analytical instruments? Here is why you should.

Two-dimensional liquid chromatography has moved from academia into industry. In this new video from LCGC TV, Kelly Zhang from Genentech discusses the next steps needed for the use of 2D LC by the pharmaceutical industry.

Performing ultrahigh-performance liquid chromatography (UHPLC) can significantly reduce analysis time without compromising on efficiency by using sub 2-μm particles that allow increased velocity while maintaining selectivity. In this new short interview, Deirdre Cabooter of KU Leuven in Belgium, discusses the advantages of using UHPLC in method development and tips to follow when transferring methods from UHPLC to HPLC instrumentation.

Two-dimensional liquid chromatography has moved from academia into industry. In this new video, Kelly Zhang of Genentech talks about the advantages gained by developing a two-dimensional LC method for analyzing impurities in pharmaceuticals, and gives practical examples.

Two-dimensional liquid chromatography has moved from academia into industry. In two new short video interviews, Kelly Zhang of Genentech talks about the advantages gained by developing a two-dimensional LC method for analyzing impurities in pharmaceuticals, as well as the challenges in developing the method.

Internal standards are an important tool of analytical chemists, yet errors in their usage are common. Mary Ellen McNally of DuPont Crop Protection offers advice for how to introduce an internal standard and how to avoid common errors with internal standard methods.

Dick Henry, consultant, discusses a trend that could renew efforts towards developing novel LC stationary phases with improved selectivity, peak symmetry, and stability. The question is, however, whether we really need more stationary phases in HPLC. Watch the video and find out.

Superficially porous particles for HPLC columns – often called "core–shell" particles have gotten a lot of attention in recent years because of their excellent performance. Yet so far, columns made with fully porous particles are still the most widely used. Will superficially porous particles eventually take over?

Monoliths have received renewed attention in the past few years. Will they - or chip-based columns - ever compete with particle-based columns? In a short video interview, Dick Henry weighs in.

In this new video clip from LCGC TV, Dwight Stoll of Gustavus Adolphus College discusses the development of new carbon-based phases for 2D LC that are well characterized and understood.

2D LC has huge potential to increase the resolving power of separations involving complex samples. In this new video clip, Dwight Stoll of Gustavus Adolphus College considers if this technique is ready to be adopted by chromatographers working in industry.

In this new video interview, Dwight Stoll of Gustavus Adolphus provides advice about key decisions in method development, common errors to avoid, and other advice for getting started with 2D LC.

Two-dimensional liquid chromatography (2D LC) is a powerful technique, but method development is more involved and time-consuming. So for what types of analyses does it make sense to invest the extra effort to develop a 2D LC method instead of a 1D method? Dwight Stoll explains.

Sequential-elution LC is a new methodology for the analysis of complex samples. In a new video initiative from LCGC, Joe Foley, Professor of Chemistry at Drexel University, Philadelphia, Pennsylvania, USA, explains very briefly how it works and its advantages over conducting multiple runs of conventional LC.