Analytical Theory

Pop quiz—can you define resolution? Would you be surprised at the number of correct answers? Which one is the best for chromatographers? In this LCGC Blog, we look at the various ways resolution can be interpreted scientifically.

Understanding the relationship between a number of variables and analysis time, and their effects on other choices made during method development, is helpful for developing methods that are both effective and time-efficient.

As part of the upcoming Recent Advances in Gas Chromatography meeting, Paul O'Nion of RSSL will provide his perspective on different elements in the periodic table.

An examination of how greening analytical methods is directly connected to greening the sample preparation step

Pop quiz – can you define resolution? Would you be surprised at the number of correct answers? Which one is the best for chromatographers? In this LCGC Blog, we look at the various ways resolution can be interpreted scientifically.

A recent study reveals that pesticide residues in herbs have a low transfer rate to herbal infusions, ensuring safer consumption of these products. The validated analytical method used in the study provides valuable insights into the levels of pesticide residues in herbs and their limited presence in the resulting infusions.

In his plenary lecture at HPLC 2023, Michal Holčapek presented his team's findings on how chromatography can help scientists record the human lipodome and better our understanding of diseases.

Metal organic frameworks (MOFs) and ionic liquids (ILs) combine to form a powerful gas chromatography stationary phase, enabling improved separation properties and expanded application possibilities. This innovative system offers enhanced separation capabilities and paves the way for advancements in gas chromatography analysis.

A new way to control ion transports in microfluidic systems has the potential to improve the technology and expand applications.

In this LCGC blog, we reflect on the movement to make analytical chemistry more environmentally friendly, and how to potentially better those efforts for the industry's future.

Developing a short list of likely causes of retention-time problems in reversed-phase LC makes troubleshooting easier.

A kinetic plot is a powerful tool, but how do you construct one—from either experimental data or data from other sources? We explain.

When applying Golay’s equation for height equivalent of a theoretical plate (HETP) to capillary GC, you should ask three key questions.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

Kinetic plots can help us understand how different combinations of parameters will perform in relation the time needed to acquire a particular column efficiency—and thus resolution.

We are all familiar with Golay’s equation relating to height equivalent to a theoretical plate (HETP) in GC. But do we understand it correctly?

Are we ready to let software and instruments take over the art of separations?

This article explains how statistical overlap theory can be applied to chromatography in everyday usage.

The statistical overlap theory (SOT) of chromatography relates the number of peaks that appear in a chromatogram to the number of detectable components and the peak capacity.

Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation?

Click here to view the complete E-Separation Solutions newsletter from February 7, 2013

Light-scattering detection: the theory and the advantages