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Multidimensional Gas Chromatography: Benefits and Considerations for Current and Prospective UsersWhen should multidimensional GC approaches be used for analyzing complex samples and analytes?
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Essentials of LC Troubleshooting, Part II, Continued: More Misbehaving Retention TimesWhen can analyte retention deviate from what is expected or normal? We explain three subtle causes.
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Characterization of Styrene–Butadiene Block Copolymers by Gel Permeation ChromatographyThe authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.
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Comparing the Chemical Profiles of Plant-Based and Traditional Meats Using GC–MS-Based MetabolomicsUntargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.
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A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in NeonatesThis simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
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Method Development for the Qualitative and Quantitative Analysis of Pesticides by Direct Injection Liquid Chromatography– Tandem Mass Spectrometry (LC–MS/MS)This modification of ASTM method D8026 for pesticides in environmental matrices includes more pesticides and lowers the reporting limits, thus increasing throughput and measurement capacity for a large surface-water monitoring project.
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Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
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The Importance of Complete Overlapping of Analyte and Internal Standard Peaks in Eliminating Matrix Effects with Liquid Chromatography–Mass Spectrometry (LC–MS)Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.
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Purification and Isolation of Cannabinoids: Current Challenges and PerspectivesThe current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
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Analysis of Accutrace Plus Marker in Fuels with GC–MSA new method has been developed to provide a sensitive, accurate and convenient GC–MS method to align with the new European regulation in relation to the introduction of the new Accutrace Plus marker for fuels.
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An LC–MS/MS Method for the Evaluation of Intestinal Permeability in Blood PlasmaA rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.
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Gas Chromatography–Mass Spectrometry (GC–MS) with Cold Electron Ionization (EI): Bridging the Gap Between GC–MS and LC–MSGas chromatography–mass spectrometry (GC–MS) with cold electron ionization (EI) is based on interfacing the GC and MS instruments with supersonic molecular beams (SMB) along with electron ionization of vibrationally cold sample compounds in SMB in a fly-through ion source (hence the name cold EI). GC–MS with cold EI improves all the central performance aspects of GC–MS. These aspects include enhanced molecular ions, improved sample identification, an extended range of compounds amenable for analysis, uniform response to all analytes, faster analysis, greater selectivity, and lower detection limits. In GC–MS with cold EI, the GC elution temperatures can be significantly lowered by reducing the column length and increasing the carrier gas flow rate. Furthermore, the injector temperature can be reduced using a high column flow rate, and sample degradation at the cold EI fly-through ion source is eliminated. Thus, a greater range of thermally labile and low volatility compounds can be analyzed. The extension of the range of compounds and applications amenable for analysis is the most important benefit of cold EI that bridges the gap with LC–MS. Several examples of GC–MS with cold EI applications are discussed including cannabinoids analysis, synthetic organic compounds analysis, and lipids in blood analysis for medical diagnostics.
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Reimagined Lab Management for the Lab of the FutureDiscover an easy to implement alternative to LIMS that combines data management, workflow, and sample management.
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The Role of Ion Pairing Agents in Liquid Chromatography (LC) SeparationsWhen used appropriately in RPLC, ion pairing agents can increase the separation and retention of charged analytes. But should they be explored in other modes of HPLC as well?
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Changing carrier gas from Helium to HydrogenThis application note highlights steps you need to consider when changing your carrier gas from helium to hydrogen.
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Development of Highly Sensitive LC–MS and CE–MS Methods for In-Depth Proteomic and Glycomic Profiling of Limited Biological SamplesBetter sample preparation and miniaturized separations are enhancing these analyses.
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A Standardized 2D-LC Screening Platform for Peak Purity Determination in Pharmaceutical AnalysisThis study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.
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5 Things to Consider When Selecting a Chromatography SilicaParticularly in the pharmaceutical industry, drug purity isn’t just a goal – it’s essential for achieving safety, stability and efficacy. However, purification is easier said than done, especially with challenging molecules like DNA and RNA “oligonucleotides,” due in large part to their diversity and the range of impurities that can be generated during production. Enter DAVISIL® chromatographic silica, with a wide range of pore diameters and particle sizes to meet your specific application, performance and sustainability requirements. Before you choose the chromatography resin for your next purification application, take a look at these 5 considerations.
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Supercritical Fluid Chromatography for Chiral Analysis, Part 2: ApplicationsIn the second part of this review article, the recent progress in SFC for enantiomeric separations is evaluated. Several applications reported on the enantioselective separation of drugs and pharmaceutical compounds using chiral SFC are discussed, including pharmaceutical applications, clinical research, forensic toxicology, and environmental sciences.
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mRNA Characterization, from 5’ Cap to Poly (A): What Ion-Pair Reversed-Phase Liquid Chromatography (IP-RPLC) Analysis Can Tell YouThis article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.
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Analysis of PFAS in Locally Acquired Food ContainersThe combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.
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Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
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Benefits of Integrating Travelling Wave Ion Mobility Spectrometry into Liquid Chromatography and Mass Spectrometry Workflows for Steroid AnalysisThe advantages provided by the implementation of ion mobility spectrometry (IMS), and in particular travelling wave ion mobility spectrometry (TWIMS), in traditional liquid chromatography–mass spectrometry (LC–MS) systems are discussed.
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Challenges in the Analytical Characterization of VLPs Through HPLC-Based MethodsThis article discusses the challenges and effective solutions for high performance liquid chromatography (HPLC)-based analytical characterization of virus-like particles (VLPs).
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Superheated Water: A New Look at Chromatographic Eluents for Reversed-Phase HPLCThe authors describe the results they've achieved by using water heated to 100–240 ºC as a liquid eluent for reversed-phase HPLC instead of an organic modifier. They point out that this alternative avoids many of the problems – toxicity, flammability, and cost – associated with organic modifiers.
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Tackling PFAS Complexity with HRMS and Bioanalytical TechniquesThere are many benefits of using high-resolution mass spectrometry (HRMS) in analyzing PFAS accumulating in living organisms. This article shows how HRMS, when combined with exposure-relevant mixtures, can help elucidate more about PFAS toxicity and exposure.
