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Playing with Selectivity for Optimal Chiral SeparationIn this study, various ways of achieving resolution by playing with selectivity are presented.
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EPA 608.3: SPE of Organochlorine Pesticides and PCBsA modified Solid Phase Extraction (SPE) method using UCT’s C18 cartridges has been demonstrated for the extraction of organochlorine pesticides and PCBs in water.
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Analysis of N-Glycans Using HPAEC-PAD/MSEfficient separation N-glycans and isomers by HPAEC and quantification with Pulsed Amperometric Detection (PAD) and MS for identification.
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When van Deemter Goes Upside Down in Chiral ChromatographyThe occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.
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Application of a Risk‑Based Approach to the Migration of Validated Test MethodsThe advantages of a risk-based approach to instrument replacement are discussed.
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Mother Liquor ManagementThis application note demonstrates the potential of valorizing mother liquor using Centrifugal Partition Chromatography (CPC) to remediate minor cannabinoids.
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Determination of Polychlorinated Biphenyls in Water Samples Using a Needle Trap Device Combined with Gas ChromatographyThe method presented here, which uses a novel needle trap device (NTD) with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.
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Separation Science: The State of the Art: The Future of Column Packing TechnologyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
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Multivariate Optimization Procedure for Dynamic Headspace Extractions Coupled to GC(×GC)A generalized approach using DoE is presented for the optimization of DHS extraction parameters.
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Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical WorkflowsCompact instrumentation offers important advantages for many workflows, as illustrated by these examples.
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Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic FactorDisplacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.
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Whither Gas Chromatography? New Tools ~ New SolutionsWe might well ask “Where is gas chromatography (GC) heading?” For many analysts, the answer may be just “more of the same,” reflecting that GC is mature and that most analysis tasks and sample types have been tried and tested. In this scenario, any changes to the basic method may be marginal—sample introduction, and maybe a new detector? But beneath this status quo is an undercurrent of passion, excitement, and power.
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Separation Science: The State of the Art: New Frontiers in Multidimensional Liquid ChromatographyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
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Mass Spectrometry–Based Process Analytical Technologies for Cell TherapiesEven compared to other biopharmaceuticals, chimeric antigen receptor (CAR) T-cell therapies are particularly complex. Mass spectrometry (MS)–based process analytical technologies can be a powerful tool in identifying and monitoring key attributes throughout the manufacturing process.
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Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary SelectivityThis review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
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Dispersive Solid-Phase Extraction and Solid-Phase Extraction for ppt-level PFAS Analysis in Apples: A ComparisonPer- and polyfluoralkyl substances (PFAS) are found in our food. Sensitive, precise, and accurate analytical methods are needed to estimate human exposure to these chemicals. A comparative study was performed between two extraction and cleanup methods (solid-phase extraction [SPE] and dispersive SPE) for the analysis of PFAS in apples. Both methods showed excellent sensitivity, precision, and accuracy. dSPE has some benefits over conventional SPE, and vice versa. The advantages and disadvantages of both methods are discussed.
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On the Surprising Retention Order of Ketamine Analogs Using a Biphenyl Stationary PhaseAn unexpected retention order for ketamine analogs was observed when using a biphenyl stationary phase for liquid chromatography-mass spectrometry (LC–MS).
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Supplementary Information: Accelerating Monoclonal Antibody Quality Control: The Role of LC–MS in Upstream BioprocessingThis information is supplementary to the article “Accelerating Monoclonal Antibody Quality Control: The Role of LC–MS in Upstream Bioprocessing”, which was published in the May 2025 issue of Current Trends in Mass Spectrometry.
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Physicochemical Methods for Vectors and Ancillary Materials in Cellular and Gene TherapiesCellular and gene therapies (CGTs) have contributed significantly to the improvement of clinical outcomes for patients in the recent years. This paper discusses a range of physicochemical methods that play an important role in the difficult characterization of viral vectors, to meet the unique needs of CGT manufacturing process development, process and product characterization, and the quality control testing of these materials.
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Column Overload in Porous Layer Open Tubular (PLOT) ColumnsThe basic types of column overload (volume and mass) and the variables that most affect the sample loading capacity in PLOT column chromatography are examined.
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Micro-Pillar Array Column Separations for Proteomics of Liver OrganoidsA micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.
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Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide ProfilingAn overview of mRNA-based vaccine production and a discussion on the methods that are currently in use to check the quality of these vaccines.
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Development and Validation of Novel Gas Chromatography (GC) and Thin Layer Chromatography (TLC) Densitometry Methods for the Quantification of Stigmasterol 3-O-β-D-Glucopyranoside (S3G) in Balanites Aegyptiaca Extract: Application to Newly Formulated Balanites CapsulesGC and TLC methods are demonstrated for quantification of stigmasterol 3-O-β-D-glucopyranoside (S3G), the main active component in the herbal nutraceutical Balanites aegyptiaca, an antihyperglycemic in Egyptian folk medicine.
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Ready for combat: Fighting against lab space shortageThe UK's life sciences prowess and ambition to become a 'science superpower' rely on the continuous supply of highly specialised lab space. However, we are currently facing a national challenge, with lab space critically low in abundance. The question is, how do we fix this problem?
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Portable and Field‑Deployable Liquid Chromatography for Environmental StudiesLiquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.
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Sonication Impacts and Sonolytic Pathways for Some Organic Solvents Used in Pharmaceutical AnalysesHeadspace GC reveals that sonication can degrade common organic solvents.
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Online Ion-Exchange Solid-Phase Extraction Cleanup for PFAS in Food of Animal OriginOnline SPE–LC–MS/MS combined with the method presented enabled automated cleanup of food extracts and determination of PFAS compounds in the ng/kg range.
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A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array DetectorThis paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.
