Author


Hai Yue

Latest:

Physicochemical Methods for Vectors and Ancillary Materials in Cellular and Gene Therapies

Cellular and gene therapies (CGTs) have contributed significantly to the improvement of clinical outcomes for patients in the recent years. This paper discusses a range of physicochemical methods that play an important role in the difficult characterization of viral vectors, to meet the unique needs of CGT manufacturing process development, process and product characterization, and the quality control testing of these materials.


Katarina Oden

Latest:

Column Overload in Porous Layer Open Tubular (PLOT) Columns

The basic types of column overload (volume and mass) and the variables that most affect the sample loading capacity in PLOT column chromatography are examined.


Lise Midtøy

Latest:

Micro-Pillar Array Column Separations for Proteomics of Liver Organoids

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.


Kris Morreel

Latest:

Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide Profiling

An overview of mRNA-based vaccine production and a discussion on the methods that are currently in use to check the quality of these vaccines.



Amira Abdel Motaal

Latest:

Development and Validation of Novel Gas Chromatography (GC) and Thin Layer Chromatography (TLC) Densitometry Methods for the Quantification of Stigmasterol 3-O-β-D-Glucopyranoside (S3G) in Balanites Aegyptiaca Extract: Application to Newly Formulated Balanites Capsules

GC and TLC methods are demonstrated for quantification of stigmasterol 3-O-β-D-glucopyranoside (S3G), the main active component in the herbal nutraceutical Balanites aegyptiaca, an antihyperglycemic in Egyptian folk medicine.


Bruntwood SciTech

Latest:

Ready for combat: Fighting against lab space shortage

The UK's life sciences prowess and ambition to become a 'science superpower' rely on the continuous supply of highly specialised lab space. However, we are currently facing a national challenge, with lab space critically low in abundance. The question is, how do we fix this problem?


Alireza Ghiasvand

Latest:

Portable and Field‑Deployable Liquid Chromatography for Environmental Studies

Liquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.


Yu (Yon) Wang

Latest:

Sonication Impacts and Sonolytic Pathways for Some Organic Solvents Used in Pharmaceutical Analyses

Headspace GC reveals that sonication can degrade common organic solvents.


Thomas Brandsch

Latest:

Online Ion-Exchange Solid-Phase Extraction Cleanup for PFAS in Food of Animal Origin

Online SPE–LC–MS/MS combined with the method presented enabled automated cleanup of food extracts and determination of PFAS compounds in the ng/kg range.


Bingfeng Li

Latest:

A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array Detector

This paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.


Wiley

Latest:

ChromTalks: The Benefit of Hindsight in Chromatography

***Thursday and Friday May 13-14 | May 20-21, 2021*** In the relaxed and unconventional talks in this symposium, highly experienced speakers from industry and academia will impart key learnings from their long experience with chromatography—from heroic failures that have taught them valuable lessons, to moments of inspirational serendipity, and everything in between. CHROMTalks will deliver lifetimes of learning in condensed form, covering topics across implementation, troubleshooting, and method development of analytical techniques. Time spent at CHROMTalks will pay back as soon as you enter your laboratory! ***Available On Demand Until May 21, 2022***


Jared Auclair

Latest:

Challenges in the Analytical Characterization of VLPs Through HPLC-Based Methods

This article discusses the challenges and effective solutions for high performance liquid chromatography (HPLC)-based analytical characterization of virus-like particles (VLPs).


Gunter Iberer

Latest:

Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents

Monoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.


Shen Huang

Latest:

Py-GC-MS Investigation of Pyrolysis Behaviours and Decomposition Products of α- and β-2,7,11-Cembratriene-4,6-Diols

The study of the thermal behaviour and pyrolysis products of these terpenoids could possibly suggest flavour precursors that could be used to provide specific flavours to tobacco.


Keon Sandra

Latest:

Hydrophobic Interaction Chromatography (HIC) for the Characterization of Therapeutic Monoclonal Antibodies and Related Products, Part 2: Practical Considerations

In the second part of this review of the current state of HIC, some practical considerations are explained, including method development, selection of the phase system, combined salt systems, and possibilities to combine HIC with other chromatographic modes.


Hanne Røberg-Larsen

Latest:

Organoids and Organ-on-a-Chip: A Need for Separation Science

Organoids are predicted to become important tools for personalized medicine and are alternatives to animal models. Separation science and mass spectrometry (MS) are key approaches for studying organoids and organ‑on-a-chip systems. Applications include the study of organoid drug metabolism and biomarker discovery.


Louis Fleck

Latest:

Extractable and Leachable Testing for Pharmaceutical Packaging, Finished Pharmaceutical Products, and Medical Devices: An Analytical Perspective

Extractable and leachable (E&L) testing for finished pharmaceutical products, bioprocess manufacturing systems, and medical devices is currently a subject of intense interest. The majority of the challenges encountered in analytical workflows are related to the highly complex matrices and relatively low analyte evaluation thresholds seen in E&L studies. This paper provide options for executing E&L testing to supporting regulatory submissions. There are multiple compliant routes possible, and the presented workflows and analytical solutions are only one of multiple successful approaches.


Siegrun Mohring

Latest:

Monitoring for Per- and Poly-Fluoroalkyl (PFAS) with Advanced Mass Spectrometry– Based Methods

Per- and poly-fluoroalkyl substances (PFAS) are a family of potentially thousands of synthetic compounds that have long been used in the manufacture of a variety of common products with stain-repellent and nonstick properties. Their signature strong fluorine and carbon bonds make them difficult to break down and, as a result, they are among the most persistent of today’s environmental pollutants. Alarmingly, PFAS can be found in drinking water and have been shown to accumulate in the body with the potential to cause multiple health problems, such as hormone disruption and cancer. Advances in mass spectrometry have facilitated the detection of known PFAS contaminants as well as the identification of poorly studied and novel compounds in watersheds. This article explores the detection of known and novel PFAS contaminants in aqueous film-forming foams and raw drinking water sources in North Carolina, using new advances in mass spectrometry and data acquisition to improve identification and quantitation.


Teknokroma Analítica

Latest:

Analysis of Omega-3 Supplements by GC-FID

FAME and cis/trans FAME composition of three omega-3 supplements have been analyzed using two diferent columns, a 100% polyethylene glycol and a 100% biscyanopropyl.


Derrick V. Gough

Latest:

Multidimensional Gas Chromatography: Benefits and Considerations for Current and Prospective Users

When should multidimensional GC approaches be used for analyzing complex samples and analytes?


Qinggang Wang

Latest:

In-Loop Analyte Degradation in Two-Dimensional Liquid Chromatography: Example and Solutions

In this installment of “LC Troubleshooting,” we describe an artifact that can arise because of compound degradation during the transfer of fractions of the first dimension (1D) column effluent to the 2D separation.


Joey Kingstad

Latest:

Novel LC–MS/MS Method with a Dual ESI and APCI Ion Source for Analysis of California-Regulated Pesticides and Mycotoxins in Medium-Chain Triglyceride (MCT) Oil Cannabis Tinctures

A novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) source was developed for analysis of 66 pesticides and 5 mycotoxins regulated by the State of California in medium-chain triglyceride (MCT) oil cannabis tinctures. The limits of quantitation (LOQs) of all of the pesticides and mycotoxins were well below California state action limits of these analytes in cannabis-based non-inhalable or edible products. A total of 62 (out of 66) pesticides and 5 mycotoxins were analyzed using LC–MS/MS with an ESI source, and the remaining 4 pesticides were determined using LC–MS/MS with an APCI source. A simple, fast, and cheap acetonitrile solvent extraction method was used for sample preparation to get good recovery and achieve high throughput for this analysis. For late-eluted analytes, a number of internal standards were used to compensate for ion suppression effects from the hydrophobic matrix.


Bram D’Haenens

Latest:

Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox

An overview of different approaches for the qualitative and quantitative analysis of polysorbates.


Erin McEvoy

Latest:

Study: Labeling Inaccuracies in Hemp-Derived CBD Products

A recent study analyzed nearly 100 CBD products to test the accuracy of advertised cannabinoid content.


Attila Felinger

Latest:

ISC 2022 Preview

The 33rd International Symposium on Chromatography (ISC 2022) will be held from 18–22 September 2022 in Budapest. Read on to find out what attendees can look forward to.


Yanchuang Feng

Latest:

Optimization of Determination Method of Cooling Agents in Cigarette Tipping Paper by Gas Chromatography

This GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.


Sarah E. Prebihalo

Latest:

Multidimensional Gas Chromatography: Benefits and Considerations for Current and Prospective Users

When should multidimensional GC approaches be used for analyzing complex samples and analytes?


Kathryn Cash

Latest:

Essentials of LC Troubleshooting, Part II, Continued: More Misbehaving Retention Times

When can analyte retention deviate from what is expected or normal? We explain three subtle causes.


A.G. Boborodea

Latest:

Characterization of Styrene–Butadiene Block Copolymers by Gel Permeation Chromatography

The authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.