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Two-Dimensional Liquid Chromatography (2D-LC): Analysis of Size-Based Heterogeneities in Monoclonal Antibody–Based Biotherapeutic ProductsWe review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.
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A Turnkey Protocol for Detecting Taste and Odor Compounds in Drinking and Surface Waters by Gas Chromatography–Mass Spectrometry and Electron Capture Detector (GC–MS/ECD)A comprehensive monitoring protocol has been developed using GC–MS/ECD in selective ion monitoring (SIM) mode, with injection performed by solid-phase microextraction (SPME) and headspace (HS). This single system has been configured to analyze for all taste and odor (T&O) compounds in Standard Method 2170, with minimal changing of columns, injectors, or SPME fibers between methods.
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An Efficient Procedure for Determining Simple Sugars in Fruit JuicesIn this column, a fast, simplified procedure for determining simple sugars in fruit juices is described.
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Effect of Mobile Phase pH and Counterion Concentration on Retention and Selectivity for a Diol Column in Hydrophilic Interaction Liquid ChromatographyThis work focuses on the effects of the mobile phase pH and the counterion concentration in buffer solution on retention in hydrophilic interaction liquid chromatography (HILIC) mode.
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Achieving Success in a Career as a Separation Scientist: An Overview of the Chinese American Chromatography Association (CACA) Career Development Workshop at PittconSince 2008, the Chinese American Chromatography Association (CACA) has hosted career development workshops at Pittcon, featuring over 65 well-established pharmaceutical, biotechnology, and contract research organizations, and academia professionals. This year’s workshop featured five speakers, all of whom shared valuable insights and advice for individuals at different stages of their careers, including graduate students, early-career professionals, high-level managers, and aspiring entrepreneurs.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography SorbentsMonoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.
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Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease DigestTrypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
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Evaluating Two Dioscin-Based Silica Stationary Phases and their Application to Achiral and Chiral SeparationsImproved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.
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Technology Trends In Separation ScienceInformative panel discussions featuring key opinion leaders from the separation science sector discussing the latest technology trends and developments in liquid chromatography, gas chromatography, sample preparation, and data handling.
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A Dream Come True: Separation Science at AnalyticaAt Analytica this year, an oral session titled “A Dream Comes True: Fantastic News from Analytical Chemistry” focused on cutting-edge techniques in separation science in Hall 5 of the International Congress Center Munich (ICM) International Congress Center Munich (ICM).
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Playing with Selectivity for Optimal Chiral SeparationIn this study, various ways of achieving resolution by playing with selectivity are presented.
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EPA 608.3: SPE of Organochlorine Pesticides and PCBsA modified Solid Phase Extraction (SPE) method using UCT’s C18 cartridges has been demonstrated for the extraction of organochlorine pesticides and PCBs in water.
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Analysis of N-Glycans Using HPAEC-PAD/MSEfficient separation N-glycans and isomers by HPAEC and quantification with Pulsed Amperometric Detection (PAD) and MS for identification.
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When van Deemter Goes Upside Down in Chiral ChromatographyThe occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.
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Application of a Risk‑Based Approach to the Migration of Validated Test MethodsThe advantages of a risk-based approach to instrument replacement are discussed.
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Mother Liquor ManagementThis application note demonstrates the potential of valorizing mother liquor using Centrifugal Partition Chromatography (CPC) to remediate minor cannabinoids.
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Determination of Polychlorinated Biphenyls in Water Samples Using a Needle Trap Device Combined with Gas ChromatographyThe method presented here, which uses a novel needle trap device (NTD) with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.
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Separation Science: The State of the Art: The Future of Column Packing TechnologyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
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Multivariate Optimization Procedure for Dynamic Headspace Extractions Coupled to GC(×GC)A generalized approach using DoE is presented for the optimization of DHS extraction parameters.
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Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical WorkflowsCompact instrumentation offers important advantages for many workflows, as illustrated by these examples.
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Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic FactorDisplacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.
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Whither Gas Chromatography? New Tools ~ New SolutionsWe might well ask “Where is gas chromatography (GC) heading?” For many analysts, the answer may be just “more of the same,” reflecting that GC is mature and that most analysis tasks and sample types have been tried and tested. In this scenario, any changes to the basic method may be marginal—sample introduction, and maybe a new detector? But beneath this status quo is an undercurrent of passion, excitement, and power.
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Separation Science: The State of the Art: New Frontiers in Multidimensional Liquid ChromatographyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
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Mass Spectrometry–Based Process Analytical Technologies for Cell TherapiesEven compared to other biopharmaceuticals, chimeric antigen receptor (CAR) T-cell therapies are particularly complex. Mass spectrometry (MS)–based process analytical technologies can be a powerful tool in identifying and monitoring key attributes throughout the manufacturing process.
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Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary SelectivityThis review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
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Dispersive Solid-Phase Extraction and Solid-Phase Extraction for ppt-level PFAS Analysis in Apples: A ComparisonPer- and polyfluoralkyl substances (PFAS) are found in our food. Sensitive, precise, and accurate analytical methods are needed to estimate human exposure to these chemicals. A comparative study was performed between two extraction and cleanup methods (solid-phase extraction [SPE] and dispersive SPE) for the analysis of PFAS in apples. Both methods showed excellent sensitivity, precision, and accuracy. dSPE has some benefits over conventional SPE, and vice versa. The advantages and disadvantages of both methods are discussed.
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On the Surprising Retention Order of Ketamine Analogs Using a Biphenyl Stationary PhaseAn unexpected retention order for ketamine analogs was observed when using a biphenyl stationary phase for liquid chromatography-mass spectrometry (LC–MS).
