Author


Colleen Hall

Latest:

Ion Mobility Spectrometry A Useful Tool, But Challenges Remain

At HPLC 2023, Valerie Gabelica of the Institut Européen de Chimie et Biologie said much is still unknown about this approach to mass spectrometry.


Craig Jakes

Latest:

Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.


Laura Fichter

Latest:

LC–MS/MS for the Quantification of Tau and Phosphorylated Tau in Alzheimer’s Disease Patients’ CSF

Quantification of Tau proteoforms in cerebrospinal fluid (CSF) could be useful to diagnose neurodegenerative diseases. LC–MS/MS results can help explain the underlying molecular mechanisms of disease evolution.


Deepika Sarin

Latest:

Two-Dimensional Liquid Chromatography (2D-LC): Analysis of Size-Based Heterogeneities in Monoclonal Antibody–Based Biotherapeutic Products

We review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.


Mark Southard

Latest:

A Turnkey Protocol for Detecting Taste and Odor Compounds in Drinking and Surface Waters by Gas Chromatography–Mass Spectrometry and Electron Capture Detector (GC–MS/ECD)

A comprehensive monitoring protocol has been developed using GC–MS/ECD in selective ion monitoring (SIM) mode, with injection performed by solid-phase microextraction (SPME) and headspace (HS). This single system has been configured to analyze for all taste and odor (T&O) compounds in Standard Method 2170, with minimal changing of columns, injectors, or SPME fibers between methods.


Merlin K. L. Bicking

Latest:

An Efficient Procedure for Determining Simple Sugars in Fruit Juices

In this column, a fast, simplified procedure for determining simple sugars in fruit juices is described.


Aigu Xie

Latest:

Effect of Mobile Phase pH and Counterion Concentration on Retention and Selectivity for a Diol Column in Hydrophilic Interaction Liquid Chromatography

This work focuses on the effects of the mobile phase pH and the counterion concentration in buffer solution on retention in hydrophilic interaction liquid chromatography (HILIC) mode.



Tao Jiang

Latest:

Achieving Success in a Career as a Separation Scientist: An Overview of the Chinese American Chromatography Association (CACA) Career Development Workshop at Pittcon

Since 2008, the Chinese American Chromatography Association (CACA) has hosted career development workshops at Pittcon, featuring over 65 well-established pharmaceutical, biotechnology, and contract research organizations, and academia professionals. This year’s workshop featured five speakers, all of whom shared valuable insights and advice for individuals at different stages of their careers, including graduate students, early-career professionals, high-level managers, and aspiring entrepreneurs.


Steven W. Baertschi

Latest:

Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry Perspective

This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.


Rainer Hahn

Latest:

Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents

Monoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.


Sara Carillo

Latest:

Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.


Wen Shu

Latest:

Evaluating Two Dioscin-Based Silica Stationary Phases and their Application to Achiral and Chiral Separations

Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.


Will Wetzel

Latest:

Inside the Laboratory: The Richardson Group at the University of South Carolina

In this edition of “Inside the Laboratory,” Susan Richardson of the University of South Carolina discusses her laboratory’s work with using electron ionization and chemical ionization with gas chromatography–mass spectrometry (GC–MS) to detect DBPs in complex environmental matrices, and how her work advances environmental analysis.


Oliver J. Schmitz

Latest:

A Dream Come True: Separation Science at Analytica

At Analytica this year, an oral session titled “A Dream Comes True: Fantastic News from Analytical Chemistry” focused on cutting-edge techniques in separation science in Hall 5 of the International Congress Center Munich (ICM) International Congress Center Munich (ICM).


Bryan Tackett

Latest:

Playing with Selectivity for Optimal Chiral Separation

In this study, various ways of achieving resolution by playing with selectivity are presented.


United Chemical Technologies

Latest:

EPA 608.3: SPE of Organochlorine Pesticides and PCBs

A modified Solid Phase Extraction (SPE) method using UCT’s C18 cartridges has been demonstrated for the extraction of organochlorine pesticides and PCBs in water.


Antec Scientific

Latest:

Analysis of N-Glycans Using HPAEC-PAD/MS

Efficient separation N-glycans and isomers by HPAEC and quantification with Pulsed Amperometric Detection (PAD) and MS for identification.


Chiara De Luca

Latest:

When van Deemter Goes Upside Down in Chiral Chromatography

The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.


Uttam Ghosh

Latest:

Application of a Risk‑Based Approach to the Migration of Validated Test Methods

The advantages of a risk-based approach to instrument replacement are discussed.



RotaChrom Technologies PLC

Latest:

Mother Liquor Management

This application note demonstrates the potential of valorizing mother liquor using Centrifugal Partition Chromatography (CPC) to remediate minor cannabinoids.


Xiyue Wang

Latest:

Determination of Polychlorinated Biphenyls in Water Samples Using a Needle Trap Device Combined with Gas Chromatography

The method presented here, which uses a novel needle trap device (NTD) with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.


Ward Van Geite

Latest:

Separation Science: The State of the Art: The Future of Column Packing Technology

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.


Sheri A. Schmidt

Latest:

Multivariate Optimization Procedure for Dynamic Headspace Extractions Coupled to GC(×GC)

A generalized approach using DoE is presented for the optimization of DHS extraction parameters.


Alexis Zimmer

Latest:

Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical Workflows

Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.


Randall Rupp

Latest:

Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic Factor

Displacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.


Jane E. Hill

Latest:

Whither Gas Chromatography? New Tools ~ New Solutions

We might well ask “Where is gas chromatography (GC) heading?” For many analysts, the answer may be just “more of the same,” reflecting that GC is mature and that most analysis tasks and sample types have been tried and tested. In this scenario, any changes to the basic method may be marginal—sample introduction, and maybe a new detector? But beneath this status quo is an undercurrent of passion, excitement, and power.


Katia Arena

Latest:

Separation Science: The State of the Art: New Frontiers in Multidimensional Liquid Chromatography

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.


Richard Rogers

Latest:

Mass Spectrometry–Based Process Analytical Technologies for Cell Therapies

Even compared to other biopharmaceuticals, chimeric antigen receptor (CAR) T-cell therapies are particularly complex. Mass spectrometry (MS)–based process analytical technologies can be a powerful tool in identifying and monitoring key attributes throughout the manufacturing process.