At HPLC 2023, Valerie Gabelica of the Institut Européen de Chimie et Biologie said much is still unknown about this approach to mass spectrometry.
Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
Quantification of Tau proteoforms in cerebrospinal fluid (CSF) could be useful to diagnose neurodegenerative diseases. LC–MS/MS results can help explain the underlying molecular mechanisms of disease evolution.
We review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.
A comprehensive monitoring protocol has been developed using GC–MS/ECD in selective ion monitoring (SIM) mode, with injection performed by solid-phase microextraction (SPME) and headspace (HS). This single system has been configured to analyze for all taste and odor (T&O) compounds in Standard Method 2170, with minimal changing of columns, injectors, or SPME fibers between methods.
In this column, a fast, simplified procedure for determining simple sugars in fruit juices is described.
This work focuses on the effects of the mobile phase pH and the counterion concentration in buffer solution on retention in hydrophilic interaction liquid chromatography (HILIC) mode.
Since 2008, the Chinese American Chromatography Association (CACA) has hosted career development workshops at Pittcon, featuring over 65 well-established pharmaceutical, biotechnology, and contract research organizations, and academia professionals. This year’s workshop featured five speakers, all of whom shared valuable insights and advice for individuals at different stages of their careers, including graduate students, early-career professionals, high-level managers, and aspiring entrepreneurs.
This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
Monoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.
Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.
In this edition of “Inside the Laboratory,” Susan Richardson of the University of South Carolina discusses her laboratory’s work with using electron ionization and chemical ionization with gas chromatography–mass spectrometry (GC–MS) to detect DBPs in complex environmental matrices, and how her work advances environmental analysis.
At Analytica this year, an oral session titled “A Dream Comes True: Fantastic News from Analytical Chemistry” focused on cutting-edge techniques in separation science in Hall 5 of the International Congress Center Munich (ICM) International Congress Center Munich (ICM).
In this study, various ways of achieving resolution by playing with selectivity are presented.
A modified Solid Phase Extraction (SPE) method using UCT’s C18 cartridges has been demonstrated for the extraction of organochlorine pesticides and PCBs in water.
Efficient separation N-glycans and isomers by HPAEC and quantification with Pulsed Amperometric Detection (PAD) and MS for identification.
The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.
The advantages of a risk-based approach to instrument replacement are discussed.
This application note demonstrates the potential of valorizing mother liquor using Centrifugal Partition Chromatography (CPC) to remediate minor cannabinoids.
The method presented here, which uses a novel needle trap device (NTD) with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.
In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
A generalized approach using DoE is presented for the optimization of DHS extraction parameters.
Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.
Displacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.
We might well ask “Where is gas chromatography (GC) heading?” For many analysts, the answer may be just “more of the same,” reflecting that GC is mature and that most analysis tasks and sample types have been tried and tested. In this scenario, any changes to the basic method may be marginal—sample introduction, and maybe a new detector? But beneath this status quo is an undercurrent of passion, excitement, and power.
In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
Even compared to other biopharmaceuticals, chimeric antigen receptor (CAR) T-cell therapies are particularly complex. Mass spectrometry (MS)–based process analytical technologies can be a powerful tool in identifying and monitoring key attributes throughout the manufacturing process.