The Effects of Inner Surface Roughness of Microbore Column Blanks on Column Performance

Publication
Article
LCGC North AmericaFebruary 2000
Volume 18
Issue 2
Pages: 106–117

The efficiencies of microbore-2 columns, which are prepared from blanks that have a wide variety of inner surface roughness, drop sharply when the size of individual surface roughness features approaches the particle size of the packing material. The results suggest that two categories of packed column structure relate to the surface features and yield high and low efficiency columns. This installment of "Column Watch" discusses this conclusion in terms of the stability of an agglomerated layer of packing particles on the blank wall when subjected to shear forces during column packing.

LCGC 18(2), 106–117 (2000).
Articles in this issue
HPLC 50 mm columns on the table in the laboratory. Fast high performance liquid chromatography analysis in a chemical and microbiological laboratory.
The Effects of Inner Surface Roughness of Microbore Column Blanks on Column Performance
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Starting Out Right, Part III – The Role of the Solvent in Controlling Selectivity
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