Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents
Monoliths are chromatography sorbents cast as a homogenous phase into chromatography columns as a single, continuous piece. In contrast, regular chromatographic solvents are packed as individual particles. In this month's "Column Watch," the guest columnists compare three of these novel sorbents with a conventional packed-particle column in terms of porosity, static, and dynamic capacity. Monoliths show flow rate–independent separation efficiency and dynamic capacity as well as higher porosity than conventional columns.
LCGC 17(11), 998–1005 (1999).
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
