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Moving Toward Green and Sustainable Sample PreparationMicroextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
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In Memoriam: Michael J. Hennessey, Sr.Michael J. Hennessy, Sr., was the beloved chairman and CEO of MJH Life Sciences, parent company of The Column. Here we look back on his impressive career.
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A Simple LC–MS Multi-Analyte Method to Determine Food Additives and Caffeine in BeveragesA simple LC–MS method has been developed and validated for the simultaneous determination of 18 synthetic food additives and caffeine in soft and energy drinks, and in various alcoholic beverages. Nine food colours (tartrazine, sunset yellow FCF, azorubine, ponceau 4R, allura red AC, patent blue V, brilliant blue FCF, green S, brilliant black BN), two preservatives (sorbic and benzoic acid) and seven sweeteners (acesulfame K, aspartame, cyclamic acid, saccharin, sucralose, neohesperidin DC, neotame) were targeted food additives. The method employs reversed-phase ultra-high performance liquid chromatography (UHPLC) for analyte separation and a single quadrupole mass spectrometer for their detection. The limits of quantification were low enough to enable a reliable control of maximum limits set for some additives (Regulation [EC] No. 1333/2008). The method was applied for analysis of a wide range of samples collected at a typical supermarket: 14 soft drinks, 19 energy drinks, and 43 alcoholic beverages.
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Rapid Quantitative Analysis of Ethylene Oxide and 1,4-Dioxane in Polymeric Excipients Using SIFT-Mass SpectrometryPharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>.
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Determination of Organophosphate Esters in Water Samples Using Gas Chromatography–Mass Spectrometry (GC–MS) and Magnetic Solid-Phase Extraction (SPE) Based on Multi-Walled Carbon Nanotubes (MWCNTs)A GC–MS method, coupled with magnetic SPE with multi-walled carbon-nanotube (MWCNT)-coated iron oxide as the adsorbent, was developed for analyzing organophosphate esters in environmental water samples.
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Challenges in Obtaining Relevant Information from One- and Two-Dimensional LC ExperimentsTo address the quest for greater separation power, the chromatographic community developed comprehensive two-dimensional liquid chromatography (LCxLC). But even with LCxLC, it can still be challenging to analyze highly complex samples and obtain accurate and correct information. In this article, opportunities for optimizing methods for extracting maximum information from one-dimensional (1D)-LC and two-dimensional (2D)-LC chromatographic data are explained.
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The Potential of Automated Strategies in Microextraction Procedures Coupled to Chromatographic TechniquesRecent developments in microextraction automation has resulted in more straightforward workflows.
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Simple Purification and Ultrahigh-Pressure Liquid Chromatography–Tandem Mass Spectrometry Determination of Five Quinoxaline 1,4-dioxides and their Metabolites in Swine LiverThe UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.
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Environmental Analysis with Integrated Ion Chromatography, Titration, and Direct MeasurementUncovering the benefits of a system that hyphenates ion chromatography, titration, and direct measurement, in environmental analysis of water samples.
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Analytical Method Lifecycle of SFC Methods from Development Use to Routine QC Implementation; Supporting Small Molecule R&D and CommercializationIn this article, we review some key considerations for chromatographic technique selection and method development across the full drug process.
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Gas Chromatography Tandem Mass Spectrometry Analysis of Ethylene Oxide: An Emerged Contaminant in Seeds and SpicesTwo alternative approaches based on gas chromatography coupled to tandem mass spectrometry (GC–MS/MS) were developed to control the maximum residue limit of the banned insecticide, ethylene oxide (EtO), and its transformation product, 2-chloroethanol (2-CE).
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Advantages of Hybrid Surface Technology to Improve Chromatographic Analysis of Food, Beverages, and Dietary SupplementsThe article discusses the application of a hybrid organic/inorganic surface technology that forms a barrier between the sample and the metal surfaces of both the HPLC system and chromatographic column. Formed by a vapor deposition of an ethylene-bridged siloxane polymer on metal substrates), this technology effectively addresses common chromatographic challenges such as analyte loss, carryover, and peak tailing due to metal-analyte interactions. It improves peak symmetry and areas, as well as reproducibility, thereby not only benefiting challenging analytes but also increasing confidence in analytical results. We demonstrate the benefits of this technology through the analysis of B-group vitamins, steviol glycosides, and dextran oligosaccharides typically found in food, beverages, and dietary supplements, showcasing its critical role in improving chromatographic performance.
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LC–MS/MS for the Quantification of Tau and Phosphorylated Tau in Alzheimer’s Disease Patients’ CSFQuantification of Tau proteoforms in cerebrospinal fluid (CSF) could be useful to diagnose neurodegenerative diseases. LC–MS/MS results can help explain the underlying molecular mechanisms of disease evolution.
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Tools for Functional Assessment of BiotherapeuticsWe present the main analytical techniques for performing functional characterization of biotherapeutic products. Such assessments are particularly critical for biosimilars, where analytical testing must ensure functional comparability with the innovator product.
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Development of Stability‑Indicating Analytical Procedures by HPLC: An Overview with Best Practices (Part 1)This article is first in a series of two white papers on stability studies and testing of pharmaceuticals, which focuses on the development of stability-indicating high performance liquid chromatography (HPLC) methods for drug substances and products. It provides an overview of the fundamentals, including the traditional approaches, as well as modern trends and software tools for expediting the process. The regulatory guidance on the necessary contents of a well-written method, strategies for efficient method execution, and life cycle management of analytical procedures, are described in Part 2 of the series.
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Advances in Chromatography Using Artificial Intelligence and Machine LearningScientists from the University of Turin, Italy have learned how to combine their complementary competencies in analytical chemistry and big data analytics to achieve significant advances in food science and health.
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There is No Time to Waste: Low-Pressure Gas Chromatography– Mass Spectrometry is a Proven Solution for Fast, Sensitive, and Robust GC–MS AnalysisLow-pressure gas chromatography (LPGC)–MS should be the first option in many GC–MS applications to provide fast, sensitive, and robust analyses; and the installation, instrumentation, and implementation of LPGC is possible in any GC–MS system.
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Can Chemistry Be Accessible For Students With Visual Impairment?This article presents a multimodal approach for students with severe visual impairment or blindness, to encourage them to learn more about chromatographic processes.
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Determination of Polychlorinated Biphenyls in Water Samples Using a Needle Trap Device Combined with Gas ChromatographyThe method presented here, which uses a novel needle trap device (NTD) with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.
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Electromembrane Extraction with Vials of Conducting PolymerIn electromembrane extraction (EME), the target analyte is extracted from an aqueous sample across a supported liquid membrane (SLM) and analyzed using LC. Through the study presented here, we demonstrate the principles of the technique and test its performance.
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Analysis of Accutrace Plus Marker in Fuels with GC–MSA new method has been developed to provide a sensitive, accurate and convenient GC–MS method to align with the new European regulation in relation to the introduction of the new Accutrace Plus marker for fuels.
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Characterization of Product Related Variants in Therapeutic mAbsNavin Rauniyar and Xuemei Han of Tanvex Biopharma USA recently discussed how identifying product-related variants through characterization enables the recognition of impurities that compromise the quality and safety of drugs.
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Sonication Impacts and Sonolytic Pathways for Some Organic Solvents Used in Pharmaceutical AnalysesHeadspace GC reveals that sonication can degrade common organic solvents.
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Simultaneous Determination of 17 Residual Monomers in Adhesives by Gas Chromatography–Mass SpectrometryAdhesives often contain toxic materials like residual monomers; here, we demonstrate quantitative analysis of residual monomers in adhesives using GC–MS.
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Detangling the Complex Web of GC × GC Method Development to Support New UsersIn this article, the authors describe a simple workflow to develop a GC×GC method for a specific sample upon initial use, with the aim of decreasing the time to accomplish functional workflows for new users.
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Becoming an AI GranddaddySeparation science will be at the core of the artificial intelligence (AI) revolution in science.
