Author
Latest:
Development of a Standardized Protocol for the Classification of Column Sets in Comprehensive Two-Dimensional Gas ChromatographyThe article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
Latest:
How Much Data is Too Much? An Analysis of the Pros and Cons of High-Resolution Mass Spectral DataHRMS is widely used in chromatography for analyzing complex mixtures. However, chromatographers should take note of the exponentially larger data set that HRMS provides before deciding whether to use the technique for the analysis they are conducting.
Latest:
Enhancing Analytical Methods: Raman Spectroscopy as an Addition to your Current Analytical PipelineWebinar Date/Time: Wed, Apr 26, 2023 11:00 AM EDT
Latest:
In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes AnalysisA new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
Latest:
Reliable Separation and Efficient Group-type Quantitation of Total Petroleum Hydrocarbons (TPHs)Petroleum contamination from leaking underground storage tanks, for example, is a significant concern for both the environment and human health. Thorough characterization of the contamination is required to form appropriate risk assessments and remediation strategies, but until now, the determination of total petroleum hydrocarbons (TPHs) in soil has typically involved a convoluted and labour-intensive process. In this article, the analysis of TPH in environmental media is simplified using flow-modulated GC×GC–FID with quantitation based on pre-defined compound groupings. This approach overcomes the drawbacks of conventional solvent fractionation approaches, by eliminating the need for sample fractionation and automating data processing workflows.
Latest:
Replacing 40+ Year-Old Analytical TechniquesPharmaceutical science has used the same slow analytical quality testing techniques for more than 40 years. Dr. Fred Regnier, CTO of Novilytic and one of the world’s top analytical chemists, explains how a new method is being utilized to expedite the molecular analysis of antibodies and to eliminate sample prep and mass spectrometry.
Latest:
In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes AnalysisA new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
Latest:
Evaluating the Goodness of Instrument Calibration for Chromatography ProceduresHow good are your calibration procedures for environmental analysis methods using GC or LC? We demonstrate the overall value of a calibration measured by RSD. We also demonstrate the use of relative standard error (RSE) as a way of evaluating the quality or goodness of calibration methods.
Latest:
Micro-Pillar Array Column Separations for Proteomics of Liver OrganoidsA micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.
Latest:
Optimizing Chemical Procurement for Precise Chromatography AnalysisA concise guide to optimizing chemical procurement.
Latest:
Ion Chromatography Separation of Alkali, Alkaline Earth, Transition, Post-transition, Lanthanide, and Actinide Metal Cations in Waste SludgeThe authors developed an ion chromatography method to separate 31 cations in a single chromatographic run using inductively coupled plasma–mass spectrometry, liquid scintillation counting, and gamma counting. The high-level radioactive waste samples contained trace radionuclides in concentrations of only a few hundred disintegrations-per-minute-per-milliliter.
Latest:
The Helium Shortage & Your LabThursday May 19th, 2022 11 am EDT | 8 am PDT | 5 pm CET | 3 pm GMT Join us as we discuss the global helium shortage and the steps labs can take to ensure they are not affected by unplanned disruptions.
Latest:
Moving Toward Green and Sustainable Sample PreparationMicroextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
Latest:
In Memoriam: Michael J. Hennessey, Sr.Michael J. Hennessy, Sr., was the beloved chairman and CEO of MJH Life Sciences, parent company of The Column. Here we look back on his impressive career.
Latest:
A Simple LC–MS Multi-Analyte Method to Determine Food Additives and Caffeine in BeveragesA simple LC–MS method has been developed and validated for the simultaneous determination of 18 synthetic food additives and caffeine in soft and energy drinks, and in various alcoholic beverages. Nine food colours (tartrazine, sunset yellow FCF, azorubine, ponceau 4R, allura red AC, patent blue V, brilliant blue FCF, green S, brilliant black BN), two preservatives (sorbic and benzoic acid) and seven sweeteners (acesulfame K, aspartame, cyclamic acid, saccharin, sucralose, neohesperidin DC, neotame) were targeted food additives. The method employs reversed-phase ultra-high performance liquid chromatography (UHPLC) for analyte separation and a single quadrupole mass spectrometer for their detection. The limits of quantification were low enough to enable a reliable control of maximum limits set for some additives (Regulation [EC] No. 1333/2008). The method was applied for analysis of a wide range of samples collected at a typical supermarket: 14 soft drinks, 19 energy drinks, and 43 alcoholic beverages.
Latest:
Rapid Quantitative Analysis of Ethylene Oxide and 1,4-Dioxane in Polymeric Excipients Using SIFT-MSPharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>.
Latest:
Determination of Organophosphate Esters in Water Samples Using Gas Chromatography–Mass Spectrometry (GC–MS) and Magnetic Solid-Phase Extraction (SPE) Based on Multi-Walled Carbon Nanotubes (MWCNTs)A GC–MS method, coupled with magnetic SPE with multi-walled carbon-nanotube (MWCNT)-coated iron oxide as the adsorbent, was developed for analyzing organophosphate esters in environmental water samples.
Latest:
Challenges in Obtaining Relevant Information from One- and Two-Dimensional LC ExperimentsTo address the quest for greater separation power, the chromatographic community developed comprehensive two-dimensional liquid chromatography (LCxLC). But even with LCxLC, it can still be challenging to analyze highly complex samples and obtain accurate and correct information. In this article, opportunities for optimizing methods for extracting maximum information from one-dimensional (1D)-LC and two-dimensional (2D)-LC chromatographic data are explained.
Latest:
The Potential of Automated Strategies in Microextraction Procedures Coupled to Chromatographic TechniquesRecent developments in microextraction automation has resulted in more straightforward workflows.
Latest:
Simple Purification and Ultrahigh-Pressure Liquid Chromatography–Tandem Mass Spectrometry Determination of Five Quinoxaline 1,4-dioxides and their Metabolites in Swine LiverThe UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.
Latest:
Environmental Analysis with Integrated Ion Chromatography, Titration, and Direct MeasurementUncovering the benefits of a system that hyphenates ion chromatography, titration, and direct measurement, in environmental analysis of water samples.
Latest:
Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method ConsiderationsHydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.
Latest:
Analytical Method Lifecyle of SFC Methods from Development Use to Routine QC Implementation: Supporting Small Molecule R&D and CommercializationIn this article, we review some key considerations for chromatographic technique selection and method development across the full drug process—from early-stage active pharmaceutical ingredient (API) synthesis to routine commercial release activities.
Latest:
Gas Chromatography Tandem Mass Spectrometry Analysis of Ethylene Oxide: An Emerged Contaminant in Seeds and SpicesTwo alternative approaches based on gas chromatography coupled to tandem mass spectrometry (GC–MS/MS) were developed to control the maximum residue limit of the banned insecticide, ethylene oxide (EtO), and its transformation product, 2-chloroethanol (2-CE).
Latest:
Advantages of Hybrid Surface Technology to Improve Chromatographic Analysis of Food, Beverages, and Dietary SupplementsThe article discusses the application of a hybrid organic/inorganic surface technology that forms a barrier between the sample and the metal surfaces of both the HPLC system and chromatographic column. Formed by a vapor deposition of an ethylene-bridged siloxane polymer on metal substrates), this technology effectively addresses common chromatographic challenges such as analyte loss, carryover, and peak tailing due to metal-analyte interactions. It improves peak symmetry and areas, as well as reproducibility, thereby not only benefiting challenging analytes but also increasing confidence in analytical results. We demonstrate the benefits of this technology through the analysis of B-group vitamins, steviol glycosides, and dextran oligosaccharides typically found in food, beverages, and dietary supplements, showcasing its critical role in improving chromatographic performance.
