Craig Jakes

Craig Jakes is a research scientist at NIBRT supporting the development of the MAM workflow.

Articles

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Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest

Craig Jakes;Silvia Millán-Martín;Dan Bach Kristensen;Ken Cook;Jonathan Bones;Sara Carillo

August 1st 2023

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.

Multi-Attribute Monitoring and the Multi-Attribute Method: A Powerful Double Act for Supporting Biopharmaceutical Manufacturing

Craig Jakes;Sara Carillo;Silvia Millán-Martín;Jonathan Bones

April 1st 2022

This work compares at-line Protein A-MS with a standard multi-attribute method (MAM) workflow for analyzing product quality attributes (PQAs) during upstream processing.