Author


Guo-Xiang Hao

Latest:

A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in Neonates

This simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.


Ynze Mengerink

Latest:

Molecular Correlative Material Characterization: Advantages for Polymer Analysis Using Liquid Chromatography

This article discusses innovation around the molecular structure of polymeric materials—an indispensable part of modern society—with a specific focus on sustainability. This field of research, so-called molecular correlative material characterization (MCMC), will enhance the transition into new sustainable functional copolymers.


Fang Yuan

Latest:

High-Throughput Profiling of Long Chain Fatty Acids and Oxylipins by LC–MS

Long chain fatty acids (LCFAs) function as a source of metabolic energy, substrates for membrane biogenesis, and storage of metabolic energy. Oxylipins, oxygenated derivatives of LCFAs, regulate the activity of many cellular processes. Existing methods for the analysis of LCFAs and oxylipins have limited compound coverage and sensitivity that, therefore, prevent their application in biological studies. In this work, we developed a high-throughput LC–MS method for analysis of 51 LCFAs and oxylipins. LCFAs and oxylipins were first extracted from biological samples via solid-phase extraction. The extracted molecules were analyzed by targeted comparative metabolomics. Saturated and monounsaturated LCFAs were analyzed in single ion reaction mode, while polyunsaturated LCFAs and oxylipins were analyzed in multiple reaction monitoring mode. Using this method, we successfully quantified 31 LCFAs and oxylipins from mouse livers.


Thomas Menneteau

Latest:

Multi-Attribute Monitoring of Therapeutic mRNA by Liquid Chromatography–Mass Spectrometry

A novel method to simultaneously characterize the capping and poly(A) tail for therapeutic mRNA in a single sample preparation workflow is described.


Mina Fakhary

Latest:

A Quick and Accurate High Performance Liquid Chromatography (HPLC) Method to Determine the Amount of Trimethylamine in Fish Oil Softgels and Multivitamin Softgels Containing Fish Oil

A HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.


Oliver A.H. Jones

Latest:

Groundwater CSI: Unravelling Pollution Sources in Complex Environments with Liquid Chromatography Triple Quadrupole Mass Spectrometry

It is often very difficult to distinguish the true source of wastewater contamination, especially where wastewater treatment plants are located in agricultural areas. Robust and sensitive techniques are needed to characterize impacts where there are multiple potential sources. This study analyzed a variety of synthetic chemicals using LC–QQQ–MS to generate unique fingerprints of pollution.


Pelle Simpson

Latest:

Metabolomics Meets Machine Learning: The Future of Nature-Inspired Drug Discovery

Researchers at Enveda Biosciences are transforming nature into new medicines with their artificial intelligence (AI)-enabled industrial-scale drug discovery platform incorporating tandem mass spectrometry (MS/MS).


Thomas Moritz

Latest:

Comprehensive Small Molecule Analysis Using Trapped Ion Mobility Spectrometry for Obtaining Accurate Metabolic Profiles

This article discusses the importance of TIMS as it enhances the sensitivity, specificity, and speed of metabolite measurement, which in turn accelerates the pace of metabolomics research.



Pauline Griffeuille

Latest:

Probe Electrospray Ionization-Mass Spectrometry and Isotope Dilution Approaches for Ultrafast Measurement of Metformin

In this study, a probe electrospray ionization coupled to mass spectrometry (PESI-MS) method for ultra-rapid determination of MtF in plasma by isotope dilution was developed.


Lindsey Reynolds

Latest:

An LC–MS/MS Method for the Evaluation of Intestinal Permeability in Blood Plasma

A rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.


Lori Euler

Latest:

Efficient Protein Purification Strategies Using Multi-Well Filter Plates

Combined with chromatography media, 96-well filter plates have emerged as an efficient tool for the fractionation of small-volume protein samples.


Svea Stephan

Latest:

Purification and Isolation of Cannabinoids: Current Challenges and Perspectives

The current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.


Hongwei Hou

Latest:

Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC Analysis

Errors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.


Alberto Chisvert

Latest:

Moving Toward Green and Sustainable Sample Preparation

Microextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.


Lanlan Zhang

Latest:

Simultaneous Determination of 17 Residual Monomers in Adhesives by Gas Chromatography–Mass Spectrometry

Adhesives often contain toxic materials like residual monomers; here, we demonstrate quantitative analysis of residual monomers in adhesives using GC–MS.


Mike Chang

Latest:

Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable Waters

A hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.


Falguni Patel

Latest:

Analysis of Therapeutic Monoclonal Antibodies Using a Platform Size Exclusion-HPLC Method

In biotherapeutics manufacturing, controlling size variants of therapeutic monoclonal antibodies (mAbs) is critical for ensuring product quality.


Wade Leveille

Latest:

Improved Performance of Ultrahigh-Pressure Liquid Chromatography–Mass Spectrometry (UHPLC–MS) Hyphenated Systems

An UHPLC–MS research prototype instrument was built to improve the resolution power and the usability of conventional LC–MS hyphenated instruments for routine analyses in pharmaceutical applications.


Rachael Szafnauer

Latest:

Automated Aroma and Flavor Profiling of Honey Using High-Capacity Sorptive Extraction

The author describes the benefits of using high-capacity sorptive extraction and statistical analysis to detect authenticity markers and combat food fraud in honey samples.


Da Ren

Latest:

New Aspects in the Integration of MS Technologies in the Biopharmaceutical Industry

High-resolution mass spectrometry (HRMS) is an increasingly critical tool for identifying, characterizing, and monitoring attributes of protein-based therapeutics.


Keystone Labs Inc.

Latest:

The Importance of Reliable THC Concentration and the Role of Analytical Data

***Live Dates: Tuesday, November 10, 2020 at 2pm EST| 1pm CST| 11am PST, and Thursday, November 12, 2020 at 2pm EST| 1pm CST| 11am PST***Learn the importance of accurate and reliable THC and CBD concentration results and the tools available to provide that data from an industry insider. ***On demand available after final airing until Nov. 12, 2021***


Xiaojuan Gu

Latest:

Evaluating Two Dioscin-Based Silica Stationary Phases and their Application to Achiral and Chiral Separations

Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.


Anissa W. Wong

Latest:

Validation of Stability-Indicating HPLC Methods for Pharmaceuticals: Overview, Methodologies, and Case Studies

In the pharmaceutical industry, method validation is essential. But what are the best practices? We review regulatory requirements, validation parameters, methodologies, acceptance criteria, trends, and software tools.


Matthew Morse

Latest:

Exploring Biopharmaceutical Analysis with Compact Capillary Liquid Chromatography Instrumentation

A recent trend in the design of LC instrumentation is the move towards miniaturized and portable systems.


Claire I. Butré

Latest:

Multi-Attribute Monitoring of Therapeutic mRNA by Liquid Chromatography–Mass Spectrometry

A novel method to simultaneously characterize the capping and poly(A) tail for therapeutic mRNA in a single sample preparation workflow is described.


Markus Muller

Latest:

Long-Term Analyses with Capillary Electrophoresis

This month's installment of "CE Currents" deals with problems that may arise when using capillary electrophoresis for unattended, long-term operation, such as sample carryover, evaporation effects, capillary conditioning, capillary surface changes, buffer handling, capillary breakage, and detector lamp deterioration.


Łukasz Rajski

Latest:

Untargeted Screening and Identification of Contaminants Using Gas Chromatography Coupled to High-Resolution Accurate Mass–Mass Spectrometry: Novel Solutions for Applied Testing Laboratories

Limitations often arise when using GC with quadrupole-based mass spectrometers for detecting volatile and semivolatile contaminants. Enter HRAMS-MS.


Caitlin N. Cain

Latest:

New Perspectives on Comparative Analysis for Comprehensive Two-Dimensional Gas Chromatography

This article provides an overview of the development and current status of comparative analysis for GC×GC–TOF-MS data and how key limitations can be overcome with a novel tile-based pairwise analysis method.


Joseph J. Takarewski

Latest:

Turbulent-Flow LC for LC–MS and LC–MS–MS Bioanalysis

This month's "Directions in Discovery" gives a brief overview of turbulent- flow liquid chromatography, a technique for fast separations. Two examples demonstrate the use of the technology for high-throughput bioanalytical separations in drug discovery.