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Validation of Stability-Indicating HPLC Methods for Pharmaceuticals: Overview, Methodologies, and Case StudiesIn the pharmaceutical industry, method validation is essential. But what are the best practices? We review regulatory requirements, validation parameters, methodologies, acceptance criteria, trends, and software tools.
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Analysis of Accutrace Plus Marker in Fuels with GC–MSA new method has been developed to provide a sensitive, accurate and convenient GC–MS method to align with the new European regulation in relation to the introduction of the new Accutrace Plus marker for fuels.
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Troubleshooting LC Separations of Biomolecules, Part II: Passivation and Mobile-Phase AdditivesIf you are analyzing metal-sensitive biomolecules, and a bioinert instrument is unavailable, or insufficient, passivation or mobile-phase additives may help. Here’s how to use those solutions, with tips for avoiding potential pitfalls.
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Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease DigestTrypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
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Development of a Standardized Protocol for the Classification of Column Sets in Comprehensive Two-Dimensional Gas ChromatographyThe article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
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Innovation in Nutrition and Food Analysis: The FoodOmicsGR_Research InfrastructureFoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
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Enhance the Performance of Solid-Phase Microextraction by Exploiting Vacuum-Assisted Headspace and Multicumulative Trapping for Olive Oil CharacterizationHeadspace-solid-phase microextraction (HS-SPME) is widely used for the analysis of volatile compounds from different kinds of samples.
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Enhancing Flame Ionization Detector Capabilities with Post-Column ReactionThe incorporation of a post-column reaction using a 3D-printed, two-stage microreactor is showing groundbreaking performance improvements for flame ionization detection in many gas chromatography applications—and delivers carbon universal response.
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The Future of Biomolecule Analysis Using HPLC/UHPLCThe evolution of biomolecule analysis is discussed in this article.
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PFAS Reference MaterialsPFAS testing can be highly challenging, which is why we make it easier with our convenient portfolio of PFAS reference materials. Advance environmental and health safety with standards you can trust.
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Identification of Microplastics in Environmental Monitoring Using Pyrolysis–GC–MS AnalysisPyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
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Separation and Characterization of Macromolecules: SCM-11 Event PreviewSeparation and Characterization of Macromolecules 11 (SCM-11) will take place from 22–24 January 2025 in Amsterdam, The Netherlands.
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ChromTalks: The Benefit of Hindsight in Chromatography***Thursday and Friday May 13-14 | May 20-21, 2021*** In the relaxed and unconventional talks in this symposium, highly experienced speakers from industry and academia will impart key learnings from their long experience with chromatography—from heroic failures that have taught them valuable lessons, to moments of inspirational serendipity, and everything in between. CHROMTalks will deliver lifetimes of learning in condensed form, covering topics across implementation, troubleshooting, and method development of analytical techniques. Time spent at CHROMTalks will pay back as soon as you enter your laboratory! ***Available On Demand Until May 21, 2022***
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A Suitable Therapeutic Drug Monitoring Method for Amoxicillin in Plasma by High Performance Liquid Chromatography–UV (HPLC–UV) in NeonatesThis simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
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Molecular Correlative Material Characterization: Advantages for Polymer Analysis Using Liquid ChromatographyThis article discusses innovation around the molecular structure of polymeric materials—an indispensable part of modern society—with a specific focus on sustainability. This field of research, so-called molecular correlative material characterization (MCMC), will enhance the transition into new sustainable functional copolymers.
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High-Throughput Profiling of Long Chain Fatty Acids and Oxylipins by LC–MSLong chain fatty acids (LCFAs) function as a source of metabolic energy, substrates for membrane biogenesis, and storage of metabolic energy. Oxylipins, oxygenated derivatives of LCFAs, regulate the activity of many cellular processes. Existing methods for the analysis of LCFAs and oxylipins have limited compound coverage and sensitivity that, therefore, prevent their application in biological studies. In this work, we developed a high-throughput LC–MS method for analysis of 51 LCFAs and oxylipins. LCFAs and oxylipins were first extracted from biological samples via solid-phase extraction. The extracted molecules were analyzed by targeted comparative metabolomics. Saturated and monounsaturated LCFAs were analyzed in single ion reaction mode, while polyunsaturated LCFAs and oxylipins were analyzed in multiple reaction monitoring mode. Using this method, we successfully quantified 31 LCFAs and oxylipins from mouse livers.
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Advancing Gene Therapy: Enzyme Selection for Effective RNA Oligonucleotide MappingNew gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), continue to make progress in both primate and first-in-human trials. As this progress builds, the industry remains accountable for characterizing these molecules to meet the requirements of regulatory authorities.
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A Quick and Accurate High Performance Liquid Chromatography (HPLC) Method to Determine the Amount of Trimethylamine in Fish Oil Softgels and Multivitamin Softgels Containing Fish OilA HPLC method was developed to measure trimethylamine in fish oil supplements, to determine if the level of this compound affects product quality attributes such as rancidity.
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Groundwater CSI: Unravelling Pollution Sources in Complex Environments with Liquid Chromatography Triple Quadrupole Mass SpectrometryIt is often very difficult to distinguish the true source of wastewater contamination, especially where wastewater treatment plants are located in agricultural areas. Robust and sensitive techniques are needed to characterize impacts where there are multiple potential sources. This study analyzed a variety of synthetic chemicals using LC–QQQ–MS to generate unique fingerprints of pollution.
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Metabolomics Meets Machine Learning: The Future of Nature-Inspired Drug DiscoveryResearchers at Enveda Biosciences are transforming nature into new medicines with their artificial intelligence (AI)-enabled industrial-scale drug discovery platform incorporating tandem mass spectrometry (MS/MS).
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Comprehensive Small Molecule Analysis Using Trapped Ion Mobility Spectrometry for Obtaining Accurate Metabolic ProfilesThis article discusses the importance of TIMS as it enhances the sensitivity, specificity, and speed of metabolite measurement, which in turn accelerates the pace of metabolomics research.
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Stainless Steel Surfaces in LC Systems, Part II – Passivation and Practical RecommendationsHow can internal LC surfaces be made less reactive?
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Probe Electrospray Ionization-Mass Spectrometry and Isotope Dilution Approaches for Ultrafast Measurement of MetforminIn this study, a probe electrospray ionization coupled to mass spectrometry (PESI-MS) method for ultra-rapid determination of MtF in plasma by isotope dilution was developed.
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An LC–MS/MS Method for the Evaluation of Intestinal Permeability in Blood PlasmaA rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.
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Efficient Protein Purification Strategies Using Multi-Well Filter PlatesCombined with chromatography media, 96-well filter plates have emerged as an efficient tool for the fractionation of small-volume protein samples.
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Purification and Isolation of Cannabinoids: Current Challenges and PerspectivesThe current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
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Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
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Moving Toward Green and Sustainable Sample PreparationMicroextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
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Simultaneous Determination of 17 Residual Monomers in Adhesives by Gas Chromatography–Mass SpectrometryAdhesives often contain toxic materials like residual monomers; here, we demonstrate quantitative analysis of residual monomers in adhesives using GC–MS.
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Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable WatersA hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.
