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New Perspectives on Comparative Analysis for Comprehensive Two-Dimensional Gas ChromatographyThis article provides an overview of the development and current status of comparative analysis for GC×GC–TOF-MS data and how key limitations can be overcome with a novel tile-based pairwise analysis method.
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Turbulent-Flow LC for LC–MS and LC–MS–MS BioanalysisThis month's "Directions in Discovery" gives a brief overview of turbulent- flow liquid chromatography, a technique for fast separations. Two examples demonstrate the use of the technology for high-throughput bioanalytical separations in drug discovery.
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Towards Automated Routine Analysis of the Distribution of Trace Elements in Single Cells Using ICP-MSAnalysis of the compositional variation in living cells is essential for understanding biological processes. Single-cell elemental analysis by triple-quadrupole ICP-MS is emerging as a selective, highly sensitive, and potentially high-throughput technique for the study of constitutive elements, and uptake of metallodrugs (or metal-containing nanomaterials) in single cells.
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Explore a Novel E3technology for Proteome AnalysisWebinar Date/Time: Thu, Dec 7, 2023 1:00 PM ES
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Online Ion-Exchange Solid-Phase Extraction Cleanup for PFAS in Food of Animal OriginOnline SPE–LC–MS/MS combined with the method presented enabled automated cleanup of food extracts and determination of PFAS compounds in the ng/kg range.
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Rapid Quantitation of PFAS in Non-Potable WatersThe presence of per- and polyfluoralkyl substances (PFAS) in water is an important health and environmental concern. Liquid chromatography–mass spectrometry (LC–MS) has been established as the most suitable technology for monitoring these substances. A method is described, using EPA 8327, for PFAS analysis in groundwater, surface water, and wastewater.
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Data Integrity: A Virtual Symposium***Live: Day One: Wednesday, February 10, 2021 at 9:30am EST - 4:30pm EST *** Day Two: Thursday, February 11, 2021at 9:30am EST - 4:30pm EST *** Join us for this two-day virtual symposium on data integrity with renowned experts Bob McDowall, Chris Burgess, and Mark Newton. This event is aimed at chromatographers and quality assurance professionals who have a working knowledge of data integrity within a regulated GXP laboratory. ***On demand available after final airing until Feb. 10, 2022***
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Development of Highly Sensitive LC–MS and CE–MS Methods for In-Depth Proteomic and Glycomic Profiling of Limited Biological SamplesBetter sample preparation and miniaturized separations are enhancing these analyses.
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Characterization of Product Related Variants in Therapeutic mAbsNavin Rauniyar and Xuemei Han of Tanvex Biopharma USA recently discussed how identifying product-related variants through characterization enables the recognition of impurities that compromise the quality and safety of drugs.
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Separation of All Classes of Carbohydrates by HPAEC-PADHigh performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) is a potential method of choice for the analysis of carbohydrates.
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Comparison of PFAS Sample Preparation Between WAX + Dispersive GCB and WAX/GCB Cartridges from Water and Soil Extracts Per Draft EPA 1633How to speed up sample prep time and equivalent acccuracy and precision.
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Aggregate and Fragment Analysis in Therapeutic Monoclonal Antibodies Using On-Line Size-Exclusion Chromatography with Native Mass SpectrometrySize-exclusion chromatography (SEC), with the use of ammonium acetate buffer, can be coupled on-line to electrospray ionization MS for the characterization of size variants of therapeutic monoclonal antibodies (mAbs). A quadrupole time-of-flight (QTOF) MS system was employed, and the MS method was optimized to achieve favorable sensitivity for high-mass detection, while maintaining the structural integrity of the aggregates (or high molecular weight species) and fragments (or low molecular weight species).
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Can Chemistry Be Accessible For Students With Visual Impairment?This article presents a multimodal approach for students with severe visual impairment or blindness, to encourage them to learn more about chromatographic processes.
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Advancing Pharmaceutical Analysis with Ion ChromatographyIon chromatography (IC) is becoming increasingly important for pharmaceutical applications. In contrast to other technologies, IC can resolve multiple ionic species and polar analytes simultaneously.
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Laser Desorption Postionization Mass SpectrometrySupplementing short pulse lasers with laser postionization increases ionization yields for desorption and ablation of solid samples in mass spectrometry. Here, we give an overview of the mechanisms and technical requirements for molecular photoionization in femtosecond (fs) laser desorption postionization mass spectrometry (LDPI-MS).
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Meeting Regulatory Needs in the Characterization of Lipid Nanoparticles for RNA Delivery via FFF-MALSA validated FFF-MALS method aligned with technical specification ISO/TS 21362 for the analysis of lipid-based nanoparticles (LNPs) encapsulating siRNA and mRNA is presented.
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Development of a Standardized Protocol for the Classification of Column Sets in Comprehensive Two-Dimensional Gas ChromatographyThe article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
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How Much Data is Too Much? An Analysis of the Pros and Cons of High-Resolution Mass Spectral DataHRMS is widely used in chromatography for analyzing complex mixtures. However, chromatographers should take note of the exponentially larger data set that HRMS provides before deciding whether to use the technique for the analysis they are conducting.
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Enhancing Analytical Methods: Raman Spectroscopy as an Addition to your Current Analytical PipelineWebinar Date/Time: Wed, Apr 26, 2023 11:00 AM EDT
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In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes AnalysisA new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
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Reliable Separation and Efficient Group-type Quantitation of Total Petroleum Hydrocarbons (TPHs)Petroleum contamination from leaking underground storage tanks, for example, is a significant concern for both the environment and human health. Thorough characterization of the contamination is required to form appropriate risk assessments and remediation strategies, but until now, the determination of total petroleum hydrocarbons (TPHs) in soil has typically involved a convoluted and labour-intensive process. In this article, the analysis of TPH in environmental media is simplified using flow-modulated GC×GC–FID with quantitation based on pre-defined compound groupings. This approach overcomes the drawbacks of conventional solvent fractionation approaches, by eliminating the need for sample fractionation and automating data processing workflows.
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Replacing 40+ Year-Old Analytical TechniquesPharmaceutical science has used the same slow analytical quality testing techniques for more than 40 years. Dr. Fred Regnier, CTO of Novilytic and one of the world’s top analytical chemists, explains how a new method is being utilized to expedite the molecular analysis of antibodies and to eliminate sample prep and mass spectrometry.
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In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes AnalysisA new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
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Evaluating the Goodness of Instrument Calibration for Chromatography ProceduresHow good are your calibration procedures for environmental analysis methods using GC or LC? We demonstrate the overall value of a calibration measured by RSD. We also demonstrate the use of relative standard error (RSE) as a way of evaluating the quality or goodness of calibration methods.
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Micro-Pillar Array Column Separations for Proteomics of Liver OrganoidsA micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.
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Optimizing Chemical Procurement for Precise Chromatography AnalysisA concise guide to optimizing chemical procurement.
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Ion Chromatography Separation of Alkali, Alkaline Earth, Transition, Post-transition, Lanthanide, and Actinide Metal Cations in Waste SludgeThe authors developed an ion chromatography method to separate 31 cations in a single chromatographic run using inductively coupled plasma–mass spectrometry, liquid scintillation counting, and gamma counting. The high-level radioactive waste samples contained trace radionuclides in concentrations of only a few hundred disintegrations-per-minute-per-milliliter.
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The Helium Shortage & Your LabThursday May 19th, 2022 11 am EDT | 8 am PDT | 5 pm CET | 3 pm GMT Join us as we discuss the global helium shortage and the steps labs can take to ensure they are not affected by unplanned disruptions.
