Analysis of the compositional variation in living cells is essential for understanding biological processes. Single-cell elemental analysis by triple-quadrupole ICP-MS is emerging as a selective, highly sensitive, and potentially high-throughput technique for the study of constitutive elements, and uptake of metallodrugs (or metal-containing nanomaterials) in single cells.
Webinar Date/Time: Thu, Dec 7, 2023 1:00 PM ES
Online SPE–LC–MS/MS combined with the method presented enabled automated cleanup of food extracts and determination of PFAS compounds in the ng/kg range.
The presence of per- and polyfluoralkyl substances (PFAS) in water is an important health and environmental concern. Liquid chromatography–mass spectrometry (LC–MS) has been established as the most suitable technology for monitoring these substances. A method is described, using EPA 8327, for PFAS analysis in groundwater, surface water, and wastewater.
***Live: Day One: Wednesday, February 10, 2021 at 9:30am EST - 4:30pm EST *** Day Two: Thursday, February 11, 2021at 9:30am EST - 4:30pm EST *** Join us for this two-day virtual symposium on data integrity with renowned experts Bob McDowall, Chris Burgess, and Mark Newton. This event is aimed at chromatographers and quality assurance professionals who have a working knowledge of data integrity within a regulated GXP laboratory. ***On demand available after final airing until Feb. 10, 2022***
Better sample preparation and miniaturized separations are enhancing these analyses.
Navin Rauniyar and Xuemei Han of Tanvex Biopharma USA recently discussed how identifying product-related variants through characterization enables the recognition of impurities that compromise the quality and safety of drugs.
High performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) is a potential method of choice for the analysis of carbohydrates.
How to speed up sample prep time and equivalent acccuracy and precision.
Size-exclusion chromatography (SEC), with the use of ammonium acetate buffer, can be coupled on-line to electrospray ionization MS for the characterization of size variants of therapeutic monoclonal antibodies (mAbs). A quadrupole time-of-flight (QTOF) MS system was employed, and the MS method was optimized to achieve favorable sensitivity for high-mass detection, while maintaining the structural integrity of the aggregates (or high molecular weight species) and fragments (or low molecular weight species).
This article presents a multimodal approach for students with severe visual impairment or blindness, to encourage them to learn more about chromatographic processes.
Ion chromatography (IC) is becoming increasingly important for pharmaceutical applications. In contrast to other technologies, IC can resolve multiple ionic species and polar analytes simultaneously.
Supplementing short pulse lasers with laser postionization increases ionization yields for desorption and ablation of solid samples in mass spectrometry. Here, we give an overview of the mechanisms and technical requirements for molecular photoionization in femtosecond (fs) laser desorption postionization mass spectrometry (LDPI-MS).
A validated FFF-MALS method aligned with technical specification ISO/TS 21362 for the analysis of lipid-based nanoparticles (LNPs) encapsulating siRNA and mRNA is presented.
The article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
HRMS is widely used in chromatography for analyzing complex mixtures. However, chromatographers should take note of the exponentially larger data set that HRMS provides before deciding whether to use the technique for the analysis they are conducting.
Webinar Date/Time: Wed, Apr 26, 2023 11:00 AM EDT
A new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
Petroleum contamination from leaking underground storage tanks, for example, is a significant concern for both the environment and human health. Thorough characterization of the contamination is required to form appropriate risk assessments and remediation strategies, but until now, the determination of total petroleum hydrocarbons (TPHs) in soil has typically involved a convoluted and labour-intensive process. In this article, the analysis of TPH in environmental media is simplified using flow-modulated GC×GC–FID with quantitation based on pre-defined compound groupings. This approach overcomes the drawbacks of conventional solvent fractionation approaches, by eliminating the need for sample fractionation and automating data processing workflows.
Pharmaceutical science has used the same slow analytical quality testing techniques for more than 40 years. Dr. Fred Regnier, CTO of Novilytic and one of the world’s top analytical chemists, explains how a new method is being utilized to expedite the molecular analysis of antibodies and to eliminate sample prep and mass spectrometry.
A new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
How good are your calibration procedures for environmental analysis methods using GC or LC? We demonstrate the overall value of a calibration measured by RSD. We also demonstrate the use of relative standard error (RSE) as a way of evaluating the quality or goodness of calibration methods.
A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.
A concise guide to optimizing chemical procurement.
The authors developed an ion chromatography method to separate 31 cations in a single chromatographic run using inductively coupled plasma–mass spectrometry, liquid scintillation counting, and gamma counting. The high-level radioactive waste samples contained trace radionuclides in concentrations of only a few hundred disintegrations-per-minute-per-milliliter.
Thursday May 19th, 2022 11 am EDT | 8 am PDT | 5 pm CET | 3 pm GMT Join us as we discuss the global helium shortage and the steps labs can take to ensure they are not affected by unplanned disruptions.
Microextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
Michael J. Hennessy, Sr., was the beloved chairman and CEO of MJH Life Sciences, parent company of The Column. Here we look back on his impressive career.