Sara Carillo

Sara Carillo is the bioanalytical research lead in the Characterisation and Comparability Laboratory in NIBRT.

Articles

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Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest

Craig Jakes;Silvia Millán-Martín;Dan Bach Kristensen;Ken Cook;Jonathan Bones;Sara Carillo

August 1st 2023

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.

High-Throughput Peptide Mapping of Monoclonal Antibodies Using Tandem Liquid Chromatography– Mass Spectrometry

Sara Carillo;Silvia Millán-Martín;Jonathan Bones

May 6th 2022

The benefits of tandem LC–MS are revealed.

Multi-Attribute Monitoring and the Multi-Attribute Method: A Powerful Double Act for Supporting Biopharmaceutical Manufacturing

Craig Jakes;Sara Carillo;Silvia Millán-Martín;Jonathan Bones

April 1st 2022

This work compares at-line Protein A-MS with a standard multi-attribute method (MAM) workflow for analyzing product quality attributes (PQAs) during upstream processing.

Exploring Capillary Electrophoresis–Mass Spectrometry for the In-Depth Intact Native Analysis of Cysteine-Conjugated Antibody-Drug Conjugates

Florian Füssl;Lisa Strasser;Sara Carillo;Jonathan Bones

October 9th 2020

This article explores the potential of microchip capillary electrophoresis–mass spectrometry (CE–MS) for the characterization of intact biopharmaceuticals, and how this technique can effectively be employed to monitor essential quality attributes of interest.