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Ion Chromatography Separation of Alkali, Alkaline Earth, Transition, Post-transition, Lanthanide, and Actinide Metal Cations in Waste SludgeThe authors developed an ion chromatography method to separate 31 cations in a single chromatographic run using inductively coupled plasma–mass spectrometry, liquid scintillation counting, and gamma counting. The high-level radioactive waste samples contained trace radionuclides in concentrations of only a few hundred disintegrations-per-minute-per-milliliter.
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Online Solid-Phase Extraction–Gas Chromatography–Flame Ionization Detection System for Monitoring Contaminants at Parts-Per-Trillion Concentrations in Process WatersOnline monitoring of odour and taste components that occur at parts-per-trillion (ppt) levels in industrial process waters requires specialized analytical hardware that is generally not compatible with the harsh environmental conditions in these typical industrial settings. An alternative instrumental method is proposed that uses dynamic extraction in combination with gas chromatography (GC) equipped with a simple flame ionization detector (FID) to achieve these extremely low detection limits.
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Aggregate and Fragment Analysis in Therapeutic Monoclonal Antibodies Using On-Line Size-Exclusion Chromatography with Native Mass SpectrometrySize-exclusion chromatography (SEC), with the use of ammonium acetate buffer, can be coupled on-line to electrospray ionization MS for the characterization of size variants of therapeutic monoclonal antibodies (mAbs). A quadrupole time-of-flight (QTOF) MS system was employed, and the MS method was optimized to achieve favorable sensitivity for high-mass detection, while maintaining the structural integrity of the aggregates (or high molecular weight species) and fragments (or low molecular weight species).
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Determination of Organophosphate Esters in Water Samples Using Gas Chromatography–Mass Spectrometry (GC–MS) and Magnetic Solid-Phase Extraction (SPE) Based on Multi-Walled Carbon Nanotubes (MWCNTs)A GC–MS method, coupled with magnetic SPE with multi-walled carbon-nanotube (MWCNT)-coated iron oxide as the adsorbent, was developed for analyzing organophosphate esters in environmental water samples.
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Exploring the Implementation of Compact Chromatographic Instrumentation in Common Analytical WorkflowsCompact instrumentation offers important advantages for many workflows, as illustrated by these examples.
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Technology Trends In Separation ScienceInformative panel discussions featuring key opinion leaders from the separation science sector discussing the latest technology trends and developments in liquid chromatography, gas chromatography, sample preparation, and data handling.
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Per- and Polyfluoroalkyl Substances (PFAS) or Interference? Using High-Resolution Mass Spectrometry as an Investigative Tool in Food AnalysisMatrix interferences can impact routine analysis with triple quadrupole methods for monitoring and quantifying PFAS in food.
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Portable and Field‑Deployable Liquid Chromatography for Environmental StudiesLiquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.
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“Signature” Analysis of Complex Environmental Samples Using Nontargeted Two‑Dimensional Gas ChromatographyNovel nontargeted analysis applications in two-dimensional gas chromatography–mass spectrometry (GC×GC–MS) and GC–high‑resolution (HR)MS have enabled the discovery of new contaminants. Now, the next frontier is nontargeted analysis for the elucidation of the mechanisms of (bio)chemical reactions in complex samples, such as the biodegradation of organics in water and soils or the conversion of plastics into oils. GC has the potential to contribute greatly to the optimization of these processes and therefore make a real contribution to net zero and circular economy agendas.
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A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array DetectorThis paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.
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Development of a Standardized Protocol for the Classification of Column Sets in Comprehensive Two-Dimensional Gas ChromatographyThe article discusses the classification and characterization of GC×GC column sets based on polarity and orthogonality, introducing a standardized approach using a reference mixture called the Century Mix to evaluate the selectivity and performance of these column sets.
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Validation of HPLC-DAD Based Stability Indicating Assay Method for Ornidazole in Periodontal Polymeric Hydrogel: Robustness Study using Quality by Design (QbD) ApproachIn the present study, a gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method has been designed and validated to quantify ornidazole (OZ) in the marketed formulation (oral gel) with the application of QbD.
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Optimization of Determination Method of Cooling Agents in Cigarette Tipping Paper by Gas ChromatographyThis GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
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Separation of Isomeric Metabolites and Gangliosides with High Performance (Drift Tube) Ion Mobility–Mass SpectrometryEmploying ion mobility at atmospheric pressure without vacuum enables coupling to high-resolution mass analyzers. High performance ion mobility–orbital trap mass spectrometry (HPIM-OT-MS) is a useful alternative for separating isomers, such as isomeric metabolites seen in drug discovery.
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Fast Semivolatiles Analysis Harmonized for EPA Methods 625.1 and 8270E Using Supported Liquid Extraction and GC–MS/MSA supported liquid extraction (SLE) and fast gas chromatography–tandem mass spectroscopy (GC–MS/MS) method, used in multiple reaction monitoring (MRM) mode, was developed for the analysis of semivolatile organic compounds (SVOCs) in environmental samples, according to the updated EPA Methods 625.1 and 8270E. This method requires minimal sample handling and yields significant throughput and productivity gains in the laboratory.
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Postnova - Separation and Sizing of a Virus Mixture Using Asymmetrical Flow Field‐Flow Fractionation Coupled to Multi‐Angle Light ScatteringBiogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). AAV are promising gene therapy delivery vehicles, whose efficacy may be negatively affected by the presence of viral aggregates. Due to its gentle separation and broad applicable size range, AF4-MALS is able to characterize AAV and their aggregates with high resolution and precision, thereby overcoming the drawbacks that column-based chromatography techniques often face when dealing with samples larger than 50 nm in size.
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Analysis of Therapeutic Monoclonal Antibodies Using a Platform Size Exclusion-HPLC MethodIn biotherapeutics manufacturing, controlling size variants of therapeutic monoclonal antibodies (mAbs) is critical for ensuring product quality.
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Fast Compound ID and Interactive 3D-Visualization of Complex High Resolution Mixture DataWednesday, March 23rd, 2022 at 11am EST | 8am PST | 4pm GMT Thursday, March 24th, 2022 at 11am CST | 12pm JST | 3am GMT Charles Minard’s historic “six-dimensional,” 2D-graphic representation of Napoleon’s disastrous 1812 march into Russia began a history of engineers and scientists realizing the value of data visualization. Today we have Investigator, a highly advanced application for reducing complex high resolution mass spectrometry data. Results are then visualized in 2D or 3D space with a large number of data dimensions, thus simplifying interpretation and speeding problem resolution.
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Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
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Development of Gas Chromatography Instruments at ShimadzuThe guest authors summarize the history of this Japanese scientific instrument company and events in the development of its products.
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Development of a “Freeze-Pour” Sample Preparation Method for the GC Analysis of Semivolatile Flavouring Chemicals Present in E-cigarette Refill LiquidsHS-GC analysis is presented as an excellent method for the analysis of high volatile components in e-liquids. For the analysis of semivolatile ingredients, an additional sample preparation step is proposed based on a LLE followed by a freeze-out of the matrix components.
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THC Isomers in Cannabis: An EAS Interview with Brandy YoungBrandy Young, PhD, an analytical chemist with more than 15 years of experience, entered the cannabis industry in response to a recognized need for laboratory testing, aligning her expertise with the emerging demand in New York's active medical and adult-use cannabis programs. Here, she shares some background information on her Eastern Analytical Symposium (EAS) talk, which delves into the characterization of tetrahydrocannabinol (THC) isomers in cannabis products.
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Evaluating Two Dioscin-Based Silica Stationary Phases and their Application to Achiral and Chiral SeparationsImproved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.
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The Importance of Complete Overlapping of Analyte and Internal Standard Peaks in Eliminating Matrix Effects with Liquid Chromatography–Mass Spectrometry (LC–MS)Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.
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Volatile Organic Compound and Sensory Profiles of Alcoholic Versus Non-Alcoholic Beer Using Immersive TF-SPME and SBSENon-alcoholic beers are becoming more popular and need to reflect the sophisticated flavourings of beers that are brewed containing alcohol. Sophisticated analytical techniques can help to achieve this.
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Volatile Organic Compound and Sensory Profiles of Alcoholic Versus Non-Alcoholic Beer Using Immersive TF-SPME and SBSENon-alcoholic beers are becoming more popular and need to reflect the sophisticated flavourings of beers that are brewed containing alcohol. Sophisticated analytical techniques can help to achieve this.
