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Determination of Microplastic Mass Content by Thermal Extraction Desorption Gas Chromatography–Mass SpectrometryA novel analysis technique has been developed for the determination of microplastics (MPs) in complex environmental samples using thermal extraction desorption gas chromatography–mass spectrometry (TED-GC–MS).
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IEC Analysis of Oligonucleotides and Evaluation of the Effect of Changes in Mobile Phase pH on SeparationAn analytical method for the separation of oligonucleotides of different chain length by IEC using a bio-inert UHPLC system is presented.
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A Full Scan Data Review Tool to Match the Speed of Acoustic Ejection Mass SpectrometryAcoustic ejection mass spectrometry (AEMS) has recently emerged as the premier ultrahigh-throughput mass spectrometric methodology for drug discovery and related fields.
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Determination of Fipronil and Its Metabolites in Eggs and Environmental Matrices by LC–MS/MSAn improved LC–MS/MS method that can rapidly detect fipronil, an insecticide harmful to human health, is presented here for chicken eggs, feed, and soil.
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The Importance of Analytical Chemistry and Instrumental Developments for Medico-Legal and Forensic ApplicationsThe pivotal role of analytical chemistry in the medico-legal and forensic applications is explored in this article.
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The Ever-Changing World of Extractables and Leachables: Lessons learned and future challengesOctober 4, 2021 at 3pm BST | 10am EST | 4pm CEST Join global industry and regulatory experts as they discuss current hot topics in extractables and leachables (E&L) testing for pharmaceuticals, biopharmaceuticals and medical devices.
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Tips & Tricks: Influence of Sample Dispersity on the GPC/SEC Separation of Multi-Component SystemsThis instalment of Tips & Tricks focuses on whether a complete separation can be achieved using GPC/SEC and, if so, under what conditions.
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Can Automation Help Me to Achieve My Sustainability Goals?Webinar Date/Time: Fri, Oct 27, 2023 11:00 AM EDT
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Comprehensive Small Molecule Analysis Using Trapped Ion Mobility Spectrometry for Obtaining Accurate Metabolic ProfilesThis article discusses the importance of TIMS as it enhances the sensitivity, specificity, and speed of metabolite measurement, which in turn accelerates the pace of metabolomics research.
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A Turnkey Protocol for Detecting Taste and Odor Compounds in Drinking and Surface Waters by Gas Chromatography–Mass Spectrometry and Electron Capture Detector (GC–MS/ECD)A comprehensive monitoring protocol has been developed using GC–MS/ECD in selective ion monitoring (SIM) mode, with injection performed by solid-phase microextraction (SPME) and headspace (HS). This single system has been configured to analyze for all taste and odor (T&O) compounds in Standard Method 2170, with minimal changing of columns, injectors, or SPME fibers between methods.
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Optimized Explosives Analysis Using Portable Gas Chromatography–Mass Spectrometry for Battlefield ForensicsWhen explosives are encountered on the battlefield, the use of portable GC–MS is valuable for the detection and confirmatory identification of pre- and post-detonation threats. In addition, this technique provides information about the source of explosives based on the detection and identification of trace-level chemicals in the sample. The data presented here confirm this capability.
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Bioburden Testing at Various Stages of a Biomanufacturing ProcessWebinar Date/Time: Wed, Nov 15, 2023 11:00 AM EST 3:00 PM GMT | 4:00 PM BST
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The Role of Liquid Chromatography–Tandem Mass Spectrometry-Based Metabolomics in Disease BiologyExamining the role that LC–MS/MS plays in metabolomics, and metabolomics’ growing importance in the field of disease biology.
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High-Throughput Profiling of Long Chain Fatty Acids and Oxylipins by LC–MSLong chain fatty acids (LCFAs) function as a source of metabolic energy, substrates for membrane biogenesis, and storage of metabolic energy. Oxylipins, oxygenated derivatives of LCFAs, regulate the activity of many cellular processes. Existing methods for the analysis of LCFAs and oxylipins have limited compound coverage and sensitivity that, therefore, prevent their application in biological studies. In this work, we developed a high-throughput LC–MS method for analysis of 51 LCFAs and oxylipins. LCFAs and oxylipins were first extracted from biological samples via solid-phase extraction. The extracted molecules were analyzed by targeted comparative metabolomics. Saturated and monounsaturated LCFAs were analyzed in single ion reaction mode, while polyunsaturated LCFAs and oxylipins were analyzed in multiple reaction monitoring mode. Using this method, we successfully quantified 31 LCFAs and oxylipins from mouse livers.
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Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LCAnalyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.
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In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes AnalysisA new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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RAFA 2024 PreviewThe 11th International Symposium on Recent Advances in Food Analysis will be in Prague, Czech Republic from 5–8 November 2024.
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Effectiveness of High-Resolution Ion Mobility for Complex AnalysesHRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
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Comparing the Chemical Profiles of Plant-Based and Traditional Meats Using GC–MS-Based MetabolomicsUntargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.
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Deployable Mass Spectrometry for Rapid On-Site BioanalysisWith the advent of ambient ionization and portable mass spectrometers, the ability to perform rapid, on-site analysis is fast becoming a reality. We review the critical recent developments enabling this capability as well as remaining challenges that must be tackled to enable widespread adoption.
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Turbulent-Flow LC for LC–MS and LC–MS–MS BioanalysisThis month's "Directions in Discovery" gives a brief overview of turbulent- flow liquid chromatography, a technique for fast separations. Two examples demonstrate the use of the technology for high-throughput bioanalytical separations in drug discovery.
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Food Odors by Sensory Directed GC-MSLipid oxidation generates off-odors in food, edible oils, and fats. DHS or Thermal Extraction-GC-MS/O using Sensory Directed Analysis quickly identifies off-odors.
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Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant MatricesQuantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.
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Comprehensive Gas Chromatography Coupled to Simultaneous Dual Detection (TOF-MS/FID) as a Confirmatory Method for MOSH and MOAH Determination in FoodFood contamination from mineral oil saturated hydrocarbons (MOSHs) and mineral oil aromatic hydrocarbons (MOAHs) is problematic and requires a sensitive analytical technique. These contaminants were analyzed using GC×GC with flame ionization detection (FID) and time-of-flight–MS (TOF–MS) parallel dual detection. The method provides enhanced chromatographic separation, along with the full mass spectra information, and overcomes difficult interferences, resulting in reduction of false positives over conventional GC–MS methods.
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Fully Automated QuEChERS Extraction and Cleanup of Organophosphate Pesticides in Orange JuiceA fully automated quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and extract clean-up method is described for analysis of organophosphate pesticides in orange juice. This method uses GC–MS and LC–MS along with an industry standard robotic x,y,z-sampling system.
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Sonication Impacts and Sonolytic Pathways for Some Organic Solvents Used in Pharmaceutical AnalysesHeadspace GC reveals that sonication can degrade common organic solvents.
Latest:
Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant MatricesQuantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.
Latest:
Efficient Sample Preparation and High-Capacity Columns for Successful Food and Beverage AnalysisOvercoming the challenges of food and beverage analysis with ion chromatography (IC).
