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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Using the Empore EZ-Trace SPE Workstation to Perform Efficient and Reproducible Extractions of SVOCsThis application note demonstrates how the Empore EZ-Trace SPE system is used to perform multiple extractions simultaneously while achieving clean and reproducible results using EPA Method 525.3 as an example application.
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Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide ProfilingAn overview of mRNA-based vaccine production and a discussion on the methods that are currently in use to check the quality of these vaccines.
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Innovation in Nutrition and Food Analysis: The FoodOmicsGR_Research InfrastructureFoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
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Towards Automated Routine Analysis of the Distribution of Trace Elements in Single Cells Using ICP-MSAnalysis of the compositional variation in living cells is essential for understanding biological processes. Single-cell elemental analysis by triple-quadrupole ICP-MS is emerging as a selective, highly sensitive, and potentially high-throughput technique for the study of constitutive elements, and uptake of metallodrugs (or metal-containing nanomaterials) in single cells.
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Kinase Fragments Dimerize Without Oligomerization Domains, by SEC-MALSDetermination of oligomeric states is an important issue in protein chemistry. For example, self-assembly via oligomerization domains is crucial for the regulation of several protein kinases. Determination of the oligomeric state of fragments of these kinases is a means of verifying the involvement of each domain in self-assembly.
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Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary SelectivityThis review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
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Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 CellsLipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time IM‑QTOF-MS was used to characterize the HepG2 lipidome.
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Differentiating Four THC Stereoisomers Formed from Δ9-THC DegradationDiscover a reliable and robust HPLC method to separate Δ10-THC and Δ6a,10a-THC isomers produced from Δ9-THC degradation using a chiral HPLC stationary phase.
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Methods for the Passivation of HPLC Instruments and ColumnsNonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.
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Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
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The Role of Separation Techniques in the Analysis of mRNA Therapeutic Drug Substances and Drug ProductsThis article reviews a range of techniques available for separative analysis of mRNA therapeutics, their associated impurities, and delivery vehicles.
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Identification of Microplastics in Environmental Monitoring Using Pyrolysis–GC–MS AnalysisPyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
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Effectiveness of High-Resolution Ion Mobility for Complex AnalysesHRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
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Application of Porous Layer Open Tubular Columns: Beyond Permanent GasesThe application of porous layer open tubular (PLOT) columns can be expanded beyond light compounds to separations of analytes with heavier boiling points.
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Moving Toward Green and Sustainable Sample PreparationMicroextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
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Advanced LC–MS Analysis for PFAS Analysis in EggsThe European Commission's regulation on maximum levels for certain contaminants in food highlights the need for precise and reliable methods to quantify per- and polyfluoroalkyl substances (PFAS) in various food matrices. This article discusses development and validation of a robust method for analyzing 21 PFAS compounds in chicken eggs using solid-phase extraction (SPE) and liquid chromatography–mass spectrometry (LC–MS).
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Comparing the Chemical Profiles of Plant-Based and Traditional Meats Using GC–MS-Based MetabolomicsUntargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.
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Charge Detection Mass Spectrometry: Analysis of Gene Therapy VectorsGene therapy vectors in the gigadalton (GDa) regime are beyond the range of conventional mass spectrometry. Charge-detection MS overcomes this limitation.
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Investigating Decomposition Odor in a Tropical Climate by Comprehensive Two-Dimensional Gas Chromatography (GC×GC) Coupled with Mass Spectrometry (MS) and Flame Ionization Detection (FID)Decomposing animal tissue releases volatile organic compounds (VOCs), of interest in forensic science. We describe the use of GC×GC–qMS/FID retrofitted with a reverse fill/flush (RFF) flow modulator for analyzing these VOCs in a tropical climate.
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Tools for Functional Assessment of BiotherapeuticsWe present the main analytical techniques for performing functional characterization of biotherapeutic products. Such assessments are particularly critical for biosimilars, where analytical testing must ensure functional comparability with the innovator product.
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Untargeted LC–MS Lipidomics with Data Independent Acquisition using Sequential Window Acquisition of All Theoretical Fragment-Ion SpectraA focus on untargeted UHPLC–MS/MS lipidomics with data independent acquisition using SWATH technology. This article explores the wide-ranging potential and peculiarities of SWATH–MS hyphenated with UHPLC for the profiling of lipidomes of various biological samples.
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VOC Monitoring in the ppqV RangeAmbient air analysis and study of the new IONICON Fusion PTR-TOF, providing ultrahigh sensitivities up to 80 000 cps/ppbV, high mass resolving power and ppqV detection limit
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Advanced LC–MS Analysis for PFAS Analysis in EggsThe European Commission's regulation on maximum levels for certain contaminants in food highlights the need for precise and reliable methods to quantify per- and polyfluoroalkyl substances (PFAS) in various food matrices. This article discusses development and validation of a robust method for analyzing 21 PFAS compounds in chicken eggs using solid-phase extraction (SPE) and liquid chromatography–mass spectrometry (LC–MS).
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Purification of Peptides and ProteinsLearn how to use flash chromatography and prep HPLC for protein purification, parameters that improve resolution, and how to select the stationary phase and solvent.
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Electromembrane Extraction with Vials of Conducting PolymerIn electromembrane extraction (EME), the target analyte is extracted from an aqueous sample across a supported liquid membrane (SLM) and analyzed using LC. Through the study presented here, we demonstrate the principles of the technique and test its performance.
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Tips & Tricks: GPC/SEC Analysis of Aqueous Polymer DispersionsPolymer dispersions are used in numerous applications including paints, binders, and adhesives. These complex mixtures usually contain polymeric components that contribute significantly to their physical and chemical properties.
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A Rapid, Automated Extraction Platform to Assess Drug Product Potency by Online Liquid Chromatography (LC)A new automated active pharmaceutical ingredient (API) extraction approach with integrated analysis by online liquid chromatography (LC) to assess drug product potency is presented.
