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A QbD with Design-of-Experiments Approach to the Development of a Chromatographic Method for the Separation of Impurities in VancomycinAnalytical methods are developed at various stages of the drug develoment process for samples of varying complexity.
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IC–ICP–MS for Monitoring the Fate of Iodinated X-ray Contrast Media after OzonationThis article shows the potential of IC–ICP–MS for monitoring iodine-containing ionic oxidation by-products that form during ozonation of iodinated X-ray contrast media.
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Analysis of Proteins, Biologics, and Nanoparticles in Biological Fluids Using Asymmetrical Flow Field-Flow FractionationAspects of applying AF4 to plasma, serum, milk, and cerebrospinal fluid in the field of analysis and characterization of proteins, biologics, and nanoparticles in biological fluids are reviewed.
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Maximizing HPLC Productivity with 2.5 μm Extended Performance (XP) ColumnsThe migration to chromatographic methods utilizing smaller particle sizes has been well demonstrated to increase productivity through faster, more efficient separations.
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The Role of Elution Gradient Shape in the Separation of Protein TherapeuticsIn this article, we discuss the role of the gradient in protein separations by reversed-phase and ionexchange high performance liquid chromatography (HPLC).
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High-Throughput Experimentation: Where Does Mass Spectrometry Fit?In the pharmaceutical industry, the use of mass spectrometry in high-throughput experimentation (HTE) has increased, thanks to the technique’s speed, sensitivity, and selectivity. We systematically evaluate the applicability of multiple MS techniques for different types of HTE samples and purposes, reviewing the pros and cons, and provide practical recommendations, Illustrated by application case studies.
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Basic Principles for Purification Using Supercritical Fluid ChromatographyAlthough supercritical fluid chromatography (SFC) is not a new technique, preparative SFC is becoming increasingly more popular with advances in instrumentation, software and chemistry.
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Empower 2 Method Validation Manager Software: A Tool for Rapid Method ValidationMethod validation is a demanding activity. It requires a large investment in personnel, materials, instruments, supervision, and, most of all, time.
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Investigating the Molecular Weight and Structure of Linear and Cross-Linked Hyaluronic Acid by SEC-MALS and SEC-Triple DetectionThis article demonstrates the application of a MALS detector within a triple detector array to measure absolute molecular weight distribution of hyaluronic acid.
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Inaccuracies in the Quantification of Peptides — A Case Study Using β-Endorphin AssayThis article describes possible sources of error that may contribute to significant inaccuracy in peptide assay methods: The fi ltration material (cellulose) used in sample clean up and the vial material (glass and plastic) used in LC analysis. This study is based on ?-endorphin but has relevance for most peptide assays.
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The Data Integrity ModelThe scope of data integrity is shown by the data integrity model, which sits within the overall pharmaceutical quality system of a regulated laboratory.
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Identifying "Known Unknowns" in Commercial Products by Mass SpectrometryThe identification of nontargeted species in environmental and commercial samples by mass spectrometry can be very difficult. In this article, authors from Eastman Chemical Company describe their systematic approach for the identification of nontargeted species using nominal and accurate mass data, searching both mass spectral and "spectra-less" databases.
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Determination of α-Amanitin in Human Serum by Solid-Phase Extracton Coupled with HPLC-UVA method is presented for the determination of ?-amanitin, a toxic cyclic octapeptide of amanitin mushrooms, in human serum by solid-phase extraction coupled with high performance liquid chromatography with UV detection.
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Determination of Phenylurea in Tap Water and Soft Drink Samples by HPLC–UV and Solid-Phase ExtractionA simple and sensitive high performance liquid chromatography (HPLC) method with ultra-violet (UV) detection has been developed for the analysis of phenylurea herbicides.
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SFC–GCxGC to Analyse Matrices from Petroleum and CoalSFC–GCxGC is shown to be useful in fossil fuel analysis. Practical examples for the analysis of complex matrices are described.
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Liquid Chromatography Methods for the Separation of Short RNA OligonucleotidesSynthetic oligonucleotides have become increasingly popular as a result of the recent discovery of ribonucleic acid interference (RNAi), a natural process for silencing gene expression.
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Modern PFAS Analysis: New Analytical Options for Testing PFAS in Various MatricesPer- and polyfuorinated alkyl substances (PFAS) are a rapidly growing environmental and human health concern. Owing to their broad commercial use, chemical stability, and bioaccumulation potential, these compounds are widely dispersed in the environment and can cause exposure through many potential pathways. To adequately estimate exposure risks, analytical methods are required that can measure low levels of PFAS compounds in many types of matrices. As will be described, recent advances in solid-phase extraction (SPE) and liquid chromatography tandem mass spectrometry (LC–MS/MS) have enabled the identification and quantification of a large number of PFAS compounds at low concentration (
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Determination of Seven Sodium Alkyl Sulphates in Environmental Water by Ion-pair Chromatography with Suppressed Conductivity DetectionA simple and fast ion-pair chromatography method to detect sodium alkyl sulphates in environmental samples using conductivity detection is described.
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Highlights of SFC 2014A review of the 8th International Symposium on Packed Column SFC (SFC 2014), which was held in Basel, Switzerland, on 8–10 October 2014.
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Application of Paper Strip Extraction in Combination with LC–MS-MS in PharmacokineticsRecent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation.
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Ionic Liquids and Their Applications in Sample PreparationThis short review covers the use of ionic liquids (ILs) and polymeric ionic liquids (PILs) in solid-phase microextraction (SPME) and dispersive microextraction (DLLME)
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HPLC/IC Method DevelopmentChromatographers are faced with a plethora of analytes, contained within a variety of matrices, for testing. The process of developing different methods can be a tricky, time-consuming process. Ade Kujore from Cecil Instruments Limited, Cambridge, UK, summarizes many of the issues that should be addressed throughout the method development process.
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Implementation of Methods Translation between Liquid Chromatography InstrumentationPharmaceutical research and development (R&D) organizations were early adopters who recognized the many benefits of UltraPerformance LC? (UPLC?) Technology including resolution, sensitivity, throughput, and productivity as compared to HPLC.
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Nonconventional Alternatives to LC–MSAn important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.
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Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative ApplicationsThis year's symposium was held in New Orleans, Louisiana, USA. In this review, Ron Majors covers HPLC and UHPLC column and sample preparation highlights, summarizes the awards presented, and reviews the overall liquid-phase chromatographic trends.
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New Method US EPA 625 with Solid Phase Extraction for Challenging WastewatersThis automated SPE methodology is a solution that will reduce analyst labor, solvent usage, and turn-around-time while maintaining the high quality results required within today's laboratories.
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Flow-Modulated Comprehensive 2D Gas Chromatography–Triple Quadrupole MS Elucidation of the Fatty Acids and Unsaponifiable Constituents of Oil Derived from Lemon Seeds, A Food-Industry Waste ProductThis article is focused on the detailed qualitative analysis of the fatty acids and the unsaponifiable constituents of a vegetable oil derived from a food-industry waste product, namely lemon seeds.
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Combining Different Stationary-Phase Chemistries to Improve the Selectivity of Impurity Profiling MethodsSerial coupling of different column types can provide several important benefits to the chromatographer, including fine-tuning selectivity to separate complex mixtures.
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The Preparative Use of Flow Field-Flow FractionationDemonstrating that flow field-flow fractionation can separate protein aggregates for preparative and analytical purposes.
