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Improving the Universal Response of Nebulization-Based UHPLC DetectionHigh performance liquid chromatography (HPLC) with light absorbance detection (UV) is limited by the dependence of detector response on the structure of the analyte. Some detection techniques based on nebulization of the mobile phase and formation of Aerosol particles demonstrate an analyte independent response that approaches "universal."
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Advanced Structural Mass Spectrometry for Systems Biology: Pulling the Needles from HaystacksIon mobility–mass spectrometry (IM-MS) is outlined as a separations method, several examples of the utility of IM-MS for complex biological measurements are illustrated, and the implications of this approach for systems biology research are discussed.
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Harmonization of Experimental Methods Used to Measure the True Hold-Up Volume of Liquid Chromatography ColumnsA harmonization of all conventional hold-up volume methods was successfully applied to RPLC, HILIC, AEX, and RP-AEX mixed-mode chromatography, regardless of the mobile phase.
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LC–MS-Compatible Separation of Polar Compounds Using Silica Hydride ColumnsA guide to using HPLC in an iterative form to clarify decision-making during method development
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Comprehensive Two-dimensional Liquid Chromatography (LC×LC): A ReviewIn this review, practical principles and guidelines for designing LCxLC methods are given.
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Advanced Structural Mass Spectrometry for Systems Biology: Pulling the Needles from HaystacksIon mobility–mass spectrometry (IM-MS) is outlined as a separations method, several examples of the utility of IM-MS for complex biological measurements are illustrated, and the implications of this approach for systems biology research are discussed.
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A QbD with Design-of-Experiments Approach to the Development of a Chromatographic Method for the Separation of Impurities in VancomycinAnalytical methods are developed at various stages of the drug develoment process for samples of varying complexity.
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IC–ICP–MS for Monitoring the Fate of Iodinated X-ray Contrast Media after OzonationThis article shows the potential of IC–ICP–MS for monitoring iodine-containing ionic oxidation by-products that form during ozonation of iodinated X-ray contrast media.
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Analysis of Proteins, Biologics, and Nanoparticles in Biological Fluids Using Asymmetrical Flow Field-Flow FractionationAspects of applying AF4 to plasma, serum, milk, and cerebrospinal fluid in the field of analysis and characterization of proteins, biologics, and nanoparticles in biological fluids are reviewed.
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Maximizing HPLC Productivity with 2.5 μm Extended Performance (XP) ColumnsThe migration to chromatographic methods utilizing smaller particle sizes has been well demonstrated to increase productivity through faster, more efficient separations.
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The Role of Elution Gradient Shape in the Separation of Protein TherapeuticsIn this article, we discuss the role of the gradient in protein separations by reversed-phase and ionexchange high performance liquid chromatography (HPLC).
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High-Throughput Experimentation: Where Does Mass Spectrometry Fit?In the pharmaceutical industry, the use of mass spectrometry in high-throughput experimentation (HTE) has increased, thanks to the technique’s speed, sensitivity, and selectivity. We systematically evaluate the applicability of multiple MS techniques for different types of HTE samples and purposes, reviewing the pros and cons, and provide practical recommendations, Illustrated by application case studies.
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Basic Principles for Purification Using Supercritical Fluid ChromatographyAlthough supercritical fluid chromatography (SFC) is not a new technique, preparative SFC is becoming increasingly more popular with advances in instrumentation, software and chemistry.
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Empower 2 Method Validation Manager Software: A Tool for Rapid Method ValidationMethod validation is a demanding activity. It requires a large investment in personnel, materials, instruments, supervision, and, most of all, time.
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Investigating the Molecular Weight and Structure of Linear and Cross-Linked Hyaluronic Acid by SEC-MALS and SEC-Triple DetectionThis article demonstrates the application of a MALS detector within a triple detector array to measure absolute molecular weight distribution of hyaluronic acid.
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Inaccuracies in the Quantification of Peptides — A Case Study Using β-Endorphin AssayThis article describes possible sources of error that may contribute to significant inaccuracy in peptide assay methods: The fi ltration material (cellulose) used in sample clean up and the vial material (glass and plastic) used in LC analysis. This study is based on ?-endorphin but has relevance for most peptide assays.
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The Data Integrity ModelThe scope of data integrity is shown by the data integrity model, which sits within the overall pharmaceutical quality system of a regulated laboratory.
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Identifying "Known Unknowns" in Commercial Products by Mass SpectrometryThe identification of nontargeted species in environmental and commercial samples by mass spectrometry can be very difficult. In this article, authors from Eastman Chemical Company describe their systematic approach for the identification of nontargeted species using nominal and accurate mass data, searching both mass spectral and "spectra-less" databases.
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Determination of α-Amanitin in Human Serum by Solid-Phase Extracton Coupled with HPLC-UVA method is presented for the determination of ?-amanitin, a toxic cyclic octapeptide of amanitin mushrooms, in human serum by solid-phase extraction coupled with high performance liquid chromatography with UV detection.
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Determination of Phenylurea in Tap Water and Soft Drink Samples by HPLC–UV and Solid-Phase ExtractionA simple and sensitive high performance liquid chromatography (HPLC) method with ultra-violet (UV) detection has been developed for the analysis of phenylurea herbicides.
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SFC–GCxGC to Analyse Matrices from Petroleum and CoalSFC–GCxGC is shown to be useful in fossil fuel analysis. Practical examples for the analysis of complex matrices are described.
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Liquid Chromatography Methods for the Separation of Short RNA OligonucleotidesSynthetic oligonucleotides have become increasingly popular as a result of the recent discovery of ribonucleic acid interference (RNAi), a natural process for silencing gene expression.
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Modern PFAS Analysis: New Analytical Options for Testing PFAS in Various MatricesPer- and polyfuorinated alkyl substances (PFAS) are a rapidly growing environmental and human health concern. Owing to their broad commercial use, chemical stability, and bioaccumulation potential, these compounds are widely dispersed in the environment and can cause exposure through many potential pathways. To adequately estimate exposure risks, analytical methods are required that can measure low levels of PFAS compounds in many types of matrices. As will be described, recent advances in solid-phase extraction (SPE) and liquid chromatography tandem mass spectrometry (LC–MS/MS) have enabled the identification and quantification of a large number of PFAS compounds at low concentration (
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Determination of Seven Sodium Alkyl Sulphates in Environmental Water by Ion-pair Chromatography with Suppressed Conductivity DetectionA simple and fast ion-pair chromatography method to detect sodium alkyl sulphates in environmental samples using conductivity detection is described.
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Highlights of SFC 2014A review of the 8th International Symposium on Packed Column SFC (SFC 2014), which was held in Basel, Switzerland, on 8–10 October 2014.
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Application of Paper Strip Extraction in Combination with LC–MS-MS in PharmacokineticsRecent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation.
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Ionic Liquids and Their Applications in Sample PreparationThis short review covers the use of ionic liquids (ILs) and polymeric ionic liquids (PILs) in solid-phase microextraction (SPME) and dispersive microextraction (DLLME)
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HPLC/IC Method DevelopmentChromatographers are faced with a plethora of analytes, contained within a variety of matrices, for testing. The process of developing different methods can be a tricky, time-consuming process. Ade Kujore from Cecil Instruments Limited, Cambridge, UK, summarizes many of the issues that should be addressed throughout the method development process.
