Author


Francesco Gasparrini

Latest:

The Evolution of Chiral Stationary Phases from HPLC to UHPLC

This article focuses on the progression that chiral stationary phases (CSPs), specifically developed for HPLC, are currently undergoing because of the pressing need of an easy switch to UHPLC.


Monika Dittmann

Latest:

Generic UHPLC Method for the Simultaneous Analysis of Compounds with a Wide Range of Polarities

There is increasing demand to analyze samples with a wide range of polarities, in fields such as environmental analysis and proteomics.


Chris Broomell

Latest:

Characterizing Antibody-Drug Conjugates for Biotherapeutics with the Light Scattering Toolkit

This article is an overview of the different types of antibody-drug conjugates (ADC) characterization that can be performed with the light scattering toolkit to support the development of biotherapeutics.


Yu Xia

Latest:

Radical Mass Spectrometry as a New Frontier for Bioanalysis

In this article, we discuss radical MS, an increasingly important area of MS development and the application to bioanalysis. At the current stage, most of the research is performed by a small set of academic groups; it is likely that these types of fundamental studies will attract more attention and even be commercialized in the near future.


Irena Vovk

Latest:

Variable Retention Times — A Case Study

This troubleshooting study of a problem of retention time variation provides a good example of how to track down the source of a problem and how to speculate on probable cause.


St. John Skilton

Latest:

The Fundamental Shift to Tandem Mass Spectrometry

In this article, we examine how tandem and tandem hybrid mass spectrometry has opened up new frontiers already. We go further and examine how lesser-known experiments are breaking new ground, with alternative fragmentation techniques, as well as the addition of extra levels of orthogonality by parallel separations techniques.



Justin Steve

Latest:

High Throughput Sub-4 Min Separation of Antibodies Using Size Exclusion Chromatography

Gel filtration chromatography (GFC) is a powerful analytical tool in the separation of antibodies. Traditionally, GFC columns with a dimension of 7.8 mm i.d. × 30 cm are used for analytical purposes.


Ilaria D’Acquarica

Latest:

The Evolution of Chiral Stationary Phases from HPLC to UHPLC

This article focuses on the progression that chiral stationary phases (CSPs), specifically developed for HPLC, are currently undergoing because of the pressing need of an easy switch to UHPLC.


R.D. Marini

Latest:

A New Method for Quality by Design Robust Optimization in Liquid Chromatography

A new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component analysis (ICA) to accurately estimate the modeled responses (that is, the retention times at the beginning, the apex, and the end) of each peak, even for coeluted peaks. This method was applied to the optimization of the separation of nine compounds in a mixture, yielding the design space and the demonstration of robustness of the method.


V. Scott Sharp

Latest:

A Multimodal Liquid and Supercritical Fluid Chromatography Chiral Separation Screening and Column Maintenance Strategy Designed to Support Molecules in Pharmaceutical Development (Part 1)

Chiral separation screening has become a widely accepted approach for the rapid identification of an appropriate chiral stationary phase for use in more focused enantioseparation optimization. A set of extended screens encompassing various chromatographic modes using HPLC and SFC is presented.




Bruce E. Richter

Latest:

Extraction of Micropollutants From Size-Limited Solid Samples

The quantitative extraction and subsequent purification of trace contaminants from (semi-)solid environmental and food matrices of regular size (that is, a few grams) is still recognized as a challenging task, typically accomplished through relatively complex off-line multistep treatment procedures. When these conventional sample preparation procedures are applied to the treatment of size-limited samples (of less than 1 g), the difficulties increase. This review discusses the different analytical strategies that can be adopted to overcome (or at least reduce) these difficulties when chromatographic techniques are involved for final instrumental determination.



Gail L. Matters

Latest:

Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma

5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.


Xiaowei Wang

Latest:

Determination of Phytohormones in Plant Extracts Using In-matrix Ethyl Chloroformate Derivatization and DLLME–GC–MS

A fast and simple sample preparation method for the simultaneous determination of 11 phytohormones in plants by GC–MS has been developed.


Milissa Flake

Latest:

Determination of Corrosion Inhibitor–Lubricity Improver in Jet Fuels by Liquid Chromatography–Electrospray Ionization Mass Spectrometry

Military jet fuel (JP-8) is very similar to commercial jet fuel (Jet A) except for the presence of three additives, fuel system icing inhibitor, corrosion inhibitor–lubricity improver (CI-LI), and antistatic additive, which are added to improve characteristics of JP-8.



David W. Johnson

Latest:

Determination of Corrosion Inhibitor–Lubricity Improver in Jet Fuels by Liquid Chromatography–Electrospray Ionization Mass Spectrometry

Military jet fuel (JP-8) is very similar to commercial jet fuel (Jet A) except for the presence of three additives, fuel system icing inhibitor, corrosion inhibitor–lubricity improver (CI-LI), and antistatic additive, which are added to improve characteristics of JP-8.



Laura McGregor

Latest:

Reliable Separation and Efficient Group-type Quantitation of Total Petroleum Hydrocarbons (TPHs)

Petroleum contamination from leaking underground storage tanks, for example, is a significant concern for both the environment and human health. Thorough characterization of the contamination is required to form appropriate risk assessments and remediation strategies, but until now, the determination of total petroleum hydrocarbons (TPHs) in soil has typically involved a convoluted and labour-intensive process. In this article, the analysis of TPH in environmental media is simplified using flow-modulated GC×GC–FID with quantitation based on pre-defined compound groupings. This approach overcomes the drawbacks of conventional solvent fractionation approaches, by eliminating the need for sample fractionation and automating data processing workflows.




Rosanne W. Slingsby

Latest:

Practical and Popular Sample Preparation Techniques for Ion Chromatography

The authors present the most common and fundamental techniques that address common matrix issues and discuss the critical chemistry considerations.


M. Gilar

Latest:

HILIC and Its Applications for Biotechnology, Part II

The modern usage and specific applications of HILIC for two other major classes of analytes - glycopeptides and glycoproteins - are discussed.


Jenny P. Dai

Latest:

A Sensitive and Cost-Effective LC–MS-MS Method for Determination of 1α,25-Dihydroxyvitamin D3 in Human Plasma

The biologically active form of vitamin D is an important analytical target in both research and clinical practice.




Jiri Urban

Latest:

In Memory of Pavel Jandera, Leading Chromatographer

Professor Pavel Jandera passed away suddenly and unexpectedly on August 5, 2021. He was one of the most famous Czech analytical chemists. He was 77 years old.