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Application of Pyrolysis–Gas Chromatography–Mass Spectrometry for the Identification of Polymeric MaterialsThe pyrolysis–GC–MS method enables direct analysis of solid or liquid polymers without sample pretreatment, as illustrated here for various materials, including a dental filling material and a car wrapping foil.
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Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry in LipidomicsAn analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described.
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Intelligent Automation of LC–MS Analysis for the Characterization of Compound LibrariesThis article reports an approach to applying intelligent automation for high-throughput LC}MS analysis of compound libraries using Microsoft Visual Basic software.
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Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry in LipidomicsAn analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.
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The Evolution of Chiral Stationary Phases from HPLC to UHPLCThis article focuses on the progression that chiral stationary phases (CSPs), specifically developed for HPLC, are currently undergoing because of the pressing need of an easy switch to UHPLC.
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Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.
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High-Resolution Mass Spectrometry: An Ideal Analytical Tool for Drug Metabolism StudiesA method using HR-MS systems to characterize the structures of metabolites is presented. The result is a general workflow for metabolism studies in drug discovery and development.
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An Enhanced Immunoaffinity Enrichment Method for Mass Spectrometry–Based Translational ProteomicsTranslational proteomics has not been as successful as originally anticipated. Because mass spectrometry (MS) can separate proteins at the sequence level, it provides the selectivity needed for this application; however, traditional challenges still exist, including time-to-result, throughput, and sample-size requirements.
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In-Capillary Sample Concentration in CEStacking and sweeping injection methods are compared and practical suggestions are provided about how to enhance sensitivity for charged and neutral compounds.
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A New Method for Quality by Design Robust Optimization in Liquid ChromatographyA new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component analysis (ICA) to accurately estimate the modeled responses (that is, the retention times at the beginning, the apex, and the end) of each peak, even for coeluted peaks. This method was applied to the optimization of the separation of nine compounds in a mixture, yielding the design space and the demonstration of robustness of the method.
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Carbon Nanotubes: Applications in Chromatography and Sample PreparationThe latest applications for carbon nanotubes in chromatography and sample preparation are outlined.
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Low Phase Ratio Stationary Phase Column Technology for the Characterization of Highly Volatile and Reactive Compounds by Gas-Liquid ChromatographyRecent advances in coating techniques and improved polymers employed as gas chromatographic stationary phases have resulted in commercializing low phase ratio capillary column technology with respectable chromatographic efficiency and inertness.
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A Sensitive and Cost-Effective LC–MS-MS Method for Determination of 1α,25-Dihydroxyvitamin D3 in Human PlasmaThe biologically active form of vitamin D is an important analytical target in both research and clinical practice.
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The Evolution of Chiral Stationary Phases from HPLC to UHPLCThis article focuses on the progression that chiral stationary phases (CSPs), specifically developed for HPLC, are currently undergoing because of the pressing need of an easy switch to UHPLC.
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Free Simulators for Virtual Chromatography Laboratory ExperimentsWhen forced to teach separation science with little or no access to a laboratory, chromatography simulators can help. Here, we describe several that are both useful and free.
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Quantitation and Characterization of Copper Plating Bath Additives by Liquid Chromatography with Charged Aerosol Detection and Electrochemical DetectionA novel approach is described for the quantitation of three additives typically used in copper plating using a dual LC system with an ECD and a CAD.
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Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.
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Determination of Sulphur-based Odourants in Commercially Available Natural Gas with Flow Modulated Comprehensive Two-Dimensional Gas ChromatographyPulsed-flow-modulated comprehensive two-dimensional gas chromatography with flame ionisation detection was used to separate and identify all common sulphur compounds found in natural gas.
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Analysis of Four Phthalate Monoesters in Human Urine Using Liquid Chromatography Tandem Mass SpectrometryA fast, sensitive and accurate quantitative method was developed using HPLC–MS–MS for analysis of phthalate metabolites in urine samples.
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Single-Compound Isotopic Analysis of Organic Materials in ArchaeologyThe authors review chromatographic separations of archaeological biomolecules for their analysis using accelerator mass spectrometry or isotope ratio mass spectrometry.
