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Analysis of Four Phthalate Monoesters in Human Urine Using Liquid Chromatography Tandem Mass SpectrometryA fast, sensitive and accurate quantitative method was developed using HPLC–MS–MS for analysis of phthalate metabolites in urine samples.
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Analysis of Four Phthalate Monoesters in Human Urine Using Liquid Chromatography Tandem Mass SpectrometryA fast, sensitive and accurate quantitative method was developed using HPLC–MS–MS for analysis of phthalate metabolites in urine samples.
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Tools for Simulation and Optimization of Separations in Ion ChromatographyComputer-based methods can simulate separations for a wide variety of analytes, columns, and eluents.
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Analysis of Active Cannabis Compounds in Edible Food Products: Gummy Bears and BrowniesPotency testing in marijuana-infused edibles is a problematic task due to the complexity of the matrices. The concentration of active ingredients in edibles can range from a few ppm to 3.5% (1). In this application, active cannabinoid compounds were extracted from gummy bears (and also brownies, results not shown), followed by HPLC analysis.
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LC–MS Sensitivity: Practical Strategies to Boost Your Signal and Lower Your NoiseVarious strategies to improve LC-MS sensitivity in order to enhance signal-to-noise ratio, and help you realize the hidden potential of this method are discussed.
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Variable Retention Times — A Case StudyThis troubleshooting study of a problem of retention time variation provides a good example of how to track down the source of a problem and how to speculate on probable cause.
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Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.
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Assay for ppm Levels of Azide in Drug SubstancesThe removal of azide as a potential impurity from a drug substance may be critical to its safety profile. The quantitation of this impurity is an important control parameter. This article describes a simple and practical assay for azide using chemical derivatization and HPLC. The method is shown to be suitable for the intended purpose on three example test materials. Potential issues for wider applications are discussed.
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Rapid and Direct Quantitation of Pharmaceutical Drugs from Urine Using MALDI-MSAn application of MALDI-MS in qualitative and quantitative analysis of pharmaceutical compounds spiked in urine is demonstrated.
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Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.
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Nontargeted Metabolite Profiling in Next-Generation Plant Breeding: A Case Study in Malting BarleyThe power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications. The metabolite extraction, detection, and analysis methods are high throughput and reproducible, and therefore, this approach represents a practical addition to the plant breeder’s molecular toolbox.
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Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry in LipidomicsAn analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described.
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Automated Solid-Phase Extraction (SPE) and GC–MS Analysis of Pond Water Samples According to EN16691 for PAHsPolycyclic aromatic hydrocarbons (PAHs) are found worldwide and are emitted from a number of sources including fossil fuel, coal and shale oil derivatives, coke production, and burning wood for home heating, and generally arise from incomplete combustion. Surface water supplies, such as water in ponds, may be used for recreational purposes or become a drinking water source. Characterization of PAHs and their concentration is of interest in maintaining public health.
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Immunoaffinity Solid-Phase Extraction with HPLC-FLD Detection for the Determination of Aflatoxins B2, B1, G2, and G1 in Ground HazelnutUsing highly specific immunoaffinity solid-phase extraction to selectively extract and enrich aflatoxins prior to HPLC-FLD analysis, without derivatization, low LOQ values-far below the European regulations-can be achieved even in highly complex and fatty matrices.
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Meeting Review: Latest Advances in the Analysis of Complex Environmental Matrices, 2019A biennial meeting jointly organized by the Environmental Chemistry Group, Separation Sciences Group, and the Water Science Forum, and discussing the latest advances in the analysis of complex environmental matrices, is now in its eighth year. The most recent iteration of the event occurred on Friday 22 February 2019 in the Science Suite, Royal Society of Chemistry, Burlington House, in London, UK. This meeting review offers an overview of what is happening in the industry.
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The Evolution of Chiral Stationary Phases from HPLC to UHPLCThis article focuses on the progression that chiral stationary phases (CSPs), specifically developed for HPLC, are currently undergoing because of the pressing need of an easy switch to UHPLC.
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Generic UHPLC Method for the Simultaneous Analysis of Compounds with a Wide Range of PolaritiesThere is increasing demand to analyze samples with a wide range of polarities, in fields such as environmental analysis and proteomics.
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Characterizing Antibody-Drug Conjugates for Biotherapeutics with the Light Scattering ToolkitThis article is an overview of the different types of antibody-drug conjugates (ADC) characterization that can be performed with the light scattering toolkit to support the development of biotherapeutics.
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Radical Mass Spectrometry as a New Frontier for BioanalysisIn this article, we discuss radical MS, an increasingly important area of MS development and the application to bioanalysis. At the current stage, most of the research is performed by a small set of academic groups; it is likely that these types of fundamental studies will attract more attention and even be commercialized in the near future.
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Variable Retention Times — A Case StudyThis troubleshooting study of a problem of retention time variation provides a good example of how to track down the source of a problem and how to speculate on probable cause.
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The Fundamental Shift to Tandem Mass SpectrometryIn this article, we examine how tandem and tandem hybrid mass spectrometry has opened up new frontiers already. We go further and examine how lesser-known experiments are breaking new ground, with alternative fragmentation techniques, as well as the addition of extra levels of orthogonality by parallel separations techniques.
