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Development of a Multi-Product SE-UHPLC Method for the Determination of Size-Variants in Bispecific Antibody FormatsThe versatile size-exclusion ultrahigh‑performance liquid chromatography (SE-UHPLC) platform method described here provides superior separation for bispecific monoclonal antibody formats compared to a previous method.
Latest:
Development of a Multi-Product SE-UHPLC Method for the Determination of Size-Variants in Bispecific Antibody FormatsThe versatile size-exclusion ultrahigh‑performance liquid chromatography (SE-UHPLC) platform method described here provides superior separation for bispecific monoclonal antibody formats compared to a previous method.
Latest:
Development of a Multi-Product SE-UHPLC Method for the Determination of Size-Variants in Bispecific Antibody FormatsThe versatile size-exclusion ultrahigh‑performance liquid chromatography (SE-UHPLC) platform method described here provides superior separation for bispecific monoclonal antibody formats compared to a previous method.
Latest:
Development of a Multi-Product SE-UHPLC Method for the Determination of Size-Variants in Bispecific Antibody FormatsThe versatile size-exclusion ultrahigh‑performance liquid chromatography (SE-UHPLC) platform method described here provides superior separation for bispecific monoclonal antibody formats compared to a previous method.
Latest:
An LC–IR Hyphenated Approach to Characterize Polymeric Excipients in Pharmaceutical FormulationsWilliam W. Carson, Ming Zhou, and Tom Kearney
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Online LC–GC: The Ultimate “Gas Chromatography Connection”In this instalment of “GC Connections”, the advantages of multidimensional chromatography with HPLC as the first dimension and GC as the second are discussed.
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Separating Sugar Isomers by HPLCSimple sugars ribose and xylose (are easily and quickly separated on a very stable amide column using high acetonitrile concentration in the mobile phase.
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Capillary Flow LC–MS Using Micro Pillar Array Columns: Combining Nano Flow Sensitivity with Analytical Flow Robustness and ThroughputIn this webcast, Dr. Jeff Op de Beeck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the PharmaFluidics µPAC capLC column can contribute to LC–MS workflow robustness. Dr. Maarten Dhaenens of ProGenTomics will show high resolution DIA data of 500 ng of human proteome analyzed using either packed bed or micro-chip based pillar array columns operated at different flow rates and gradients. In his presentation, he focuses on the many different metrics that are important to proteomics analysis and the general application of LC–MS. Live:Friday, Dec. 13, 2019 at 10am EST | 9am CST | 3pm GMT | 4pm CET On demand available after airing until Dec. 13, 2020. Register free
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Automated Peak Tracking for Comprehensive Impurity Profiling with Chemometric Mass Spectrometric Data ProcessingScreening with multiple orthogonal HPLC methods provides a comprehensive alternative to single method drug impurity profiling with their complementary selectivity. One key challenge of the approach is to track the peaks across the orthogonal chromatograms and identify all unique impurities.
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Systematic Evaluation of HILIC Stationary Phases for MS Characterization of OligonucleotidesHydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
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Organic Phase Injection for Concentrated SamplesAvoid precipitation of concentrated samples by splitting the pump channels: organic phase will pass through the autosampler; aqueous phase will join at the column head.
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Flow-Modulated Comprehensive 2D Gas Chromatography–Triple Quadrupole MS Elucidation of the Fatty Acids and Unsaponifiable Constituents of Oil Derived from Lemon Seeds, A Food-Industry Waste ProductThis article is focused on the detailed qualitative analysis of the fatty acids and the unsaponifiable constituents of a vegetable oil derived from a food-industry waste product, namely lemon seeds.
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Ion Chromatography for Small Molecule Determination in Clinical and Pharmaceutical Studies .This article presents possible uses of ion chromatography and related techniques combined with various detection methods for clinical and pharmaceutical analysis of common inorganic and organic anions and cations. An overview of achievements in this area from the past 10 years is presented and the most important trends and development perspectives for ion chromatography are described.
Latest:
Ion Chromatography for Small Molecule Determination in Clinical and Pharmaceutical Studies .This article presents possible uses of ion chromatography and related techniques combined with various detection methods for clinical and pharmaceutical analysis of common inorganic and organic anions and cations. An overview of achievements in this area from the past 10 years is presented and the most important trends and development perspectives for ion chromatography are described.
Latest:
Ion Chromatography for Small Molecule Determination in Clinical and Pharmaceutical Studies .This article presents possible uses of ion chromatography and related techniques combined with various detection methods for clinical and pharmaceutical analysis of common inorganic and organic anions and cations. An overview of achievements in this area from the past 10 years is presented and the most important trends and development perspectives for ion chromatography are described.
Latest:
Ion Chromatography for Small Molecule Determination in Clinical and Pharmaceutical Studies .This article presents possible uses of ion chromatography and related techniques combined with various detection methods for clinical and pharmaceutical analysis of common inorganic and organic anions and cations. An overview of achievements in this area from the past 10 years is presented and the most important trends and development perspectives for ion chromatography are described.
Latest:
Celebrating the Career of Professor Klaus K. UngerJ.J. Kirkland and J.J. DeStefano spoke to LCGC about the important contributions Dr. Klaus Unger has made to chromatography as Dr. Unger celebrates his 80th birthday.
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Considerations and Advances in Developing Analytical Assays for Measuring Product-Related Variants in Bispecific AntibodiesGiven the complexity of producing bispecific antibodies, suitable analytical methods to detect and measure the levels of undesired variants are essential. Here, a novel MS-based method for variant detection is presented.
Latest:
Considerations and Advances in Developing Analytical Assays for Measuring Product-Related Variants in Bispecific AntibodiesGiven the complexity of producing bispecific antibodies, suitable analytical methods to detect and measure the levels of undesired variants are essential. Here, a novel MS-based method for variant detection is presented.
Latest:
Chromatography Data Systems: Perspectives, Principles, and TrendsThis technical overview of chromatography data systems (CDS) looks at how CDS are designed, how they can be used most effectively, and what developments we can expect in the future.
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Chromatography Data Systems: Perspectives, Principles, and TrendsThis technical overview of chromatography data systems (CDS) looks at how CDS are designed, how they can be used most effectively, and what developments we can expect in the future.
Latest:
Chromatography Data Systems: Perspectives, Principles, and TrendsThis technical overview of chromatography data systems (CDS) looks at how CDS are designed, how they can be used most effectively, and what developments we can expect in the future.
Latest:
IC–ICP–MS for Monitoring the Fate of Iodinated X-ray Contrast Media after OzonationThis article shows the potential of IC–ICP–MS for monitoring iodine-containing ionic oxidation by-products that form during ozonation of iodinated X-ray contrast media.
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Determination of PAHs in Combustion-Related Samples via Multidimensional Chromatographic MethodsGas chromatography–mass spectrometry (GC–MS), reversed-phase LC with stop-flow fluorescence (FL), and constant energy synchronous fluorescence spectroscopy (CESFS) are explored to determine PAH isomers in three combustion-related standard reference materials.
Latest:
Determination of PAHs in Combustion-Related Samples via Multidimensional Chromatographic MethodsGas chromatography–mass spectrometry (GC–MS), reversed-phase LC with stop-flow fluorescence (FL), and constant energy synchronous fluorescence spectroscopy (CESFS) are explored to determine PAH isomers in three combustion-related standard reference materials.
Latest:
Determination of PAHs in Combustion-Related Samples via Multidimensional Chromatographic MethodsGas chromatography–mass spectrometry (GC–MS), reversed-phase LC with stop-flow fluorescence (FL), and constant energy synchronous fluorescence spectroscopy (CESFS) are explored to determine PAH isomers in three combustion-related standard reference materials.
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State-of-the-Art Biopharmaceutical AnalysisThe articles presented here highlight important recent developments in biopharmaceutical analysis.
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Not (Only) Reversed-Phase Liquid Chromatography–Mass Spectrometry: Alternative LC–MS ApproachesReversed-phase LC–MS has limitations in numerous analytical applications. Alternative MS-compatible chromatographic techniques separate analytes in the liquid phase based on different retention mechanisms compared with reversed-phase LC. This article describes these alternative chromatographic approaches, relevant applications, and the future of these techniques.
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Monitoring of On-column Methionine Oxidation as Part of a System Suitability Test During UHPLC–MS/MS Peptide MappingLong-term column use can lead to on-column methionine oxidation during LC–MS/MS peptide mapping of antibody-based biotherapeutics. Following the approach described here minimizes the risk of measuring oxidative artifacts, and helps generate high quality data to provide reliable quantitative information about product-related heterogeneities.
Latest:
Monitoring of On-column Methionine Oxidation as Part of a System Suitability Test During UHPLC–MS/MS Peptide MappingLong-term column use can lead to on-column methionine oxidation during LC–MS/MS peptide mapping of antibody-based biotherapeutics. Following the approach described here minimizes the risk of measuring oxidative artifacts, and helps generate high quality data to provide reliable quantitative information about product-related heterogeneities.
