Author


S. Mohammed

Latest:

Enhancing Phosphotyrosine Proteome Coverage Using a Combined ETD and CID Approach on a LTQ Orbitrap XL ETD

Collision-induced dissociation (CID) and electron transfer dissociation (ETD) are complementary mass spectrometric fragmentation techniques. We have used CID and ETD in different approaches to analyse tyrosine phosphorylation using a Thermo Scientific LTQ Orbitrap XL equipped with ETD.


Gilson, Inc.

Latest:

VERITY SKID LC: Process Scale Liquid Chromatography System

Discover how to automatically manage all the purification steps involved in process scale chromatography including generation of the elution mobile phase, the injection of samples, the detection of molecules, and the collection of fractions.


Andras Guttman

Latest:

Bioanalytical Tools for the Characterization of Biologics and Biosimilars

LC–MS and CE–MS are the go-to techniques for characterizing biologics, but often help is needed from a range of other methods.


Anne Baumann

Latest:

Electrochemistry and LC–MS for Metabolite Generation and Identification: Tools, Technologies and Trends

This article provides an introduction to electrochemical liquid chromatography mass spectrometry. The instrumental set-up is presented and selected applications in drug development processes are discussed.


Tom Cleveland

Latest:

Extend uHPLC µm Column Lifetime with KrudKatcher Ultra In-line Filters

For many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.



Markes International, Ltd.

Latest:

Trace-level analysis of VOCs in a tomato product using headspace extraction with large volume preconcentration (LVP) and multi-step enrichment (MSE)

Trace-level analysis of VOCs in a tomato product using headspace extraction with large volume preconcentration (LVP) and multi-step enrichment (MSE)


Paul Zomer

Latest:

Data Handling and Validation in Automated Detection of Food Toxicants Using Full Scan GC–MS and LC–MS

An overview of current chromatography-based food toxicant screening is presented.


Laura C. Main

Latest:

Enhanced Peptide Identification with an Ultra High Resolution Mass Spectrometer

Analysis of biological samples frequently involves the identification of peptides from low amounts of complex samples. Confident identification of these peptides requires rapid generation of high quality, high sensitivity MS and MS–MS data. The maXis TOF mass spectrometer incorporates novel technical innovations, which together produce an unprecedented level of data quality, resulting in a significant increase in the number of peptides identified from challenging samples.


Eva de les Neus Duran Giner

Latest:

Rapid LC Determination of UV Filters in Cosmetics using Ethanol as the Mobile Phase

A rapid and environmentally friendly LC method for the simultaneous determination of 12 UV filters in cosmetic samples using ethanol as the mobile phase.


Günther K. Bonn

Latest:

Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant Matrices

Quantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.


Doug Staples

Latest:

Using Comprehensive GCxGC–TOF-MS for Enhanced Detection and Separation in Antidoping Control Screening

The misuse of androgenic anabolic steroids in sports was banned in 1976 by the International Olympic Committee and global sports community. The illegal use of anabolic steroids has reached disturbing levels worldwide. This worldwide problem is fueled partially by an ever-increasing demand for better athletic performance. The World Anti-Doping Agency has formulated strict guidelines for minimum allowable concentrations of exogenous anabolic steroids and their metabolites. The standard test methods for doping control are analyzed in urine samples with trimethyl-silyl derivatization. Urine is a complex and difficult biological matrix. This research shows the advantages of using comprehensive two-dimensional gas chromatography–time-of-flight-mass spectrometry (GCÃ-GC–TOF-MS) and illustrates the capability of GCÃ-GC-TOF-MS to be an effective instrumental option for antidoping control screening.


Matthias Rainer

Latest:

Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant Matrices

Quantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.


Jun Zhu

Latest:

Simultaneous Determination of Formononentin, Biochanin A, Daidzen and Genistein in Trifolium pratense (Red Clover) by HPLC

A simple and rapid HPLC method was established to simultaneously determine the active ingredients of red clover.


Arjen Lommen

Latest:

Automated Gas and Liquid Chromatography Retention Time Modelling and Prediction Using Open-Access Molecular Database Structures and Quantitative Structure-Chromatography Retention Relationships

This article describes open access sofware for the modelling and prediction of retention times in gas and liquid chromatography. This software provides useful results for food analysis.


Stephen Brown

Latest:

Monday Tutorial Sessions

The tutorial sessions at HPLC 2018 are part of the conference’s educational mission. The sessions consist of presentations given by experts on various topics, with more background provided than might be presented in a typical 20-minute talk.


Peter Carr

Latest:

New Horizons in Reversed-Phase Chromatography

The coauthors discuss the various attributes of and approaches to reversed-phase chromatography.


Helga Trufelli

Latest:

Toward a Universal Detector for Small Molecule Applications: Direct-EI in LC–MS

This article describes the operating principles of the direct-electron ionization (EI) interface, which is becoming more popular in many LC–MS applications. Matrix effects and the role of direct-EI as a universal detector for small molecule analysis are also discussed in detail. The advantages and drawbacks of this approach are described and a comparison with atmospheric pressure ionization (API) interfaces is made. The potential of direct-EI is illustrated with a selection of practical applications.


Atis Chakrabarti

Latest:

TSKgel Amide-80, 2 m Columns for the Analysis of Glycans

Most proteins, particularly soluble and membrane-bound proteins expressed in the endoplasmic reticulum, are glycosylated. The extent and result of glycosylation varies.


Emmanuelle Cognard

Latest:

Advances in TOF-MS-Based Screening for Food Safety Residue Analysis with a Positive Approach

Mass spectrometry plays an increasingly significant role in the analysis of residues and contaminants in food. Here we will illustrate how the combination of ultrahigh-pressure liquid chromatography (UHPLC) and high-resolution time-of-flight-mass spectrometry (TOF-MS) is used to generate a screen of veterinary drug residues in products of animal origin. The use of UHPLC–TOF-MS and dedicated, workflow directed software allows rapid screening for large numbers of residues and automated quantification of positive samples. In addition, we illustrate how the data generated using MSE acquisition mode enable critical structural information to be collected, which offers additional selectivity and confirmatory data for compound identification and facilitates elucidation of the structure of newly discovered compounds.


Leandro Wang Hantao

Latest:

Revisiting the Fundamentals of Untargeted Data Analysis with Comprehensive Two-Dimensional Gas Chromatography (GC×GC): With Great Peak Capacity, There Must Also Come Great Responsibility

Two-dimensional gas chromatography (GC×GC) and chemometrics are leading the way in new strategies on multivariate data analysis. Using GC×GC in untargeted analysis reveals how far the technique has advanced the field of separation science.


Robert White

Latest:

Ultralow Detection of Estrogenic Compounds by GC–NCI-MS-MS

A number of clinical situations now call for high-sensitivity measurement of estrogens, including monitoring during female hormone replacement therapy, antiestrogen treatment, and estrogen deficiency in men. Traditional immunoassay methods and liquid chromatography–tandem mass spectrometry (LC–MS-MS) do not provide the sensitivity and selectivity required for these applications. In contrast, a gas chromatography–negative chemical ionization–tandem mass spectrometry (GC–NCI-MS-MS) platform can provide detection limits below 1 pg/mL when used in conjunction with the appropriate derivatization protocol, with very short cycle times.


Danilo Sciarrone

Latest:

Advances in Multidimensional LC–GC for Food Analysis

On-line heart-cut LC–GC and, more recently, comprehensive LC–GC (LC?GC) are very powerful analytical techniques because of the combination of the selectivity features of LC with the high efficiency of GC. This article presents an overview of the most recently used interfacing systems, as well as applications in the food analysis.


Rongjie Fu

Latest:

Analysis of Barbiturates in Urine with Agilent 6430 LC–MS–MS and Poroshell 120 EC-C18

Toxicology Laboratory at the Veterans Administration, Portland, Oregon, USA, Agilent Technologies


Pilar Franco

Latest:

Peptide Analysis: Zwitterionic Chiral Ion-Exchangers as Complementary Option to HILIC and to Reversed-Phase Chromatography

Therapeutic peptides represent one of the fastest growing segments in the pharmaceutical market. To bring these products to the market in a consistent manner, high quality is a major concern and requires stringent quality control (QC) methods. This article discusses the potential of zwitterionic chiral ion-exchangers to support peptide analysis and quality control as a flexible complementary tool to monitor the stereochemical integrity and chemical modifications.


Doug Kitson

Latest:

Country Focus: Pole Positions

Doug Kitson looks at some of Poland's leading separation science groups.


Daniel W. Armstrong

Latest:

Next Generation Peak Fitting for Separations

Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.


Mark Libarondi

Latest:

Laser Thermal Desorption and GC×GC for Harsh Environment and Planetary Mass Spectrometry

The development of analytical instrumentation for harsh terrestrial environments and outer planet space exploration exponentially increases instrument requirements-for features such as robustness, autonomous operation, and speed-and poses unique system integration challenges. Here, we explore the use of laser thermal desorption coupled to comprehensive two-dimensional gas chromatography (LTD-GC×GC) for use with a compact, high-resolution mass spectrometer for challenging applications.


Bruce Bailey

Latest:

Quantitation and Characterization of Copper Plating Bath Additives by Liquid Chromatography with Charged Aerosol Detection and Electrochemical Detection

A novel approach is described for the quantitation of three additives typically used in copper plating using a dual LC system with an ECD and a CAD.


Andreas Wieghaus

Latest:

Rapid Screening of Pesticides Using U-HPLC/MS

The screening of pesticides, mycotoxins, and veterinary drugs is of great importance in regulated environments such as food or feed analysis. Due to some of the limitations of traditional triple quadrupole approaches (for example, targeted analyte detection, limited number of compounds, and unidentified unknown compounds), there is currently a trend towards use of full-scan MS data for the analysis of residue samples. Current screening approaches mainly rely on the use of ToF instruments coupled to U-HPLC delivering mass accuracy (~5 ppm) at a maximum resolution of <15,000. This can produce inaccurate mass measurements due the presence of unresolved background matrix interferences. In this work we show a full-scan MS screening approach with the Thermo Scientific Exactive mass spectrometer, a novel single-stage Orbitrapâ„¢ MS capable of providing precise mass accuracies at resolutions of up to 100,000 without the need for internal mass calibration.