Author
Latest:
Nontargeted Screening and Accurate Mass Confirmation of Pesticides Using High-Resolution LC–Orbital Trap Mass SpectrometryThe increasing use of pesticide testing coupled with reductions in maximum permissible residue levels of pesticides in food have driven demand for fast, sensitive, and cost-effective analytical methods for high-throughput screening of multiclass pesticides in food. Detection of 510 pesticides at low parts-per-billion levels can be achieved within minutes using orbital trap technology. The high resolving power of these systems enables accurate mass confirmation of all compounds, including isobaric pesticides. This article will provide an overview of current legislation and illustrate how mass spectrometry instrumentation can enable fast and accurate pesticide screening.
Latest:
Real-Time Profiling of Volatile Malt Aldehydes Using Selected Ion Flow Tube Mass SpectrometryThe authors use headspace SIFT-MS to target and identify volatiles in various malt aldehydes. The specificity and speed are compared to current methodology.
Latest:
Novel Analytical Methods for the Discovery and Trace Analysis of Biochemically Active CompoundsNovel analytical methods for the discovery and trace analysis of biochemically active compunds in three main area are described: protein analysis, screening technologies and multidimensional separations.
Latest:
Application of Microbore UHPLC–MS-MS to the Quantitation of In Vivo Pharmacokinetic Study SamplesLiquid chromatography coupled with tandem mass spectrometry (LC–MS-MS) is the primary bioanalytical technique used today within the pharmaceutical industry for the quantitation of small molecules in biological matrices such as plasma. In recent years, chromatographic resolution has been improved with the development of ultrahigh-pressure liquid chromatography (UHPLC) systems that utilize smaller diameter particles (< 3 μm) and operate at pressures > 5000 psi. While most LC systems utilized for this application are optimized for columns with internal diameters (i.d.) between 2.0 and 4.6 mm, a new UHPLC system designed for microbore columns (≤ 1 mm i.d.) has been introduced recently. This article will discuss the advantages of using a microbore UHPLC system coupled with a tandem mass spectrometer for the quantitation of in vivo pharmacokinetic samples. These advantages include reduced sample and solvent consumption, improved chromatographic resolution and speed, and reduced mass spectrometer source..
Latest:
Liquid Chromatography–Atmospheric Pressure Photoionization-Mass Spectrometry Analysis of the Nonvolatile Precursors of Rancid Smell in MayonnaiseFor lipid-containing food products like mayonnaise, determining nonvolatile lipid oxidation products, the precursor compounds for rancidity, makes it possible to predict product shelf life at an earlier stage in product development. A method based on normal-phase liquid chromatography with atmospheric pressure photoionization-mass spectrometry (LC–APPI-MS) was developed for this purpose.
Latest:
LC Analysis of Aminoglycoside Antibiotics Kanamycin and AmikacinKanamycin and amikacin are aminoglycoside antibiotics used to treat serious bacterial infections. Amikacin is used for infections resistant to other aminoglycosides because it is less susceptible to enzymatic reactions.
Latest:
High-throughput Method Development for Aldehydes and Ketones Using an Agilent 1290 Infinity LC-systemThis application note deals with the development of a fast method for the determination of 13 aldehyde and ketone derivates with the Agilent 1290 Infinity LC-system. The method, which used acetone as organic co-solvent, separates the analytes within 3.5 minutes.
Latest:
Extend uHPLC µm Column Lifetime with KrudKatcher Ultra In-line FiltersFor many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.
Latest:
UHPLC Coupled with Fourier Transform Orbitrap for Residue AnalysisThe trend in residue analysis has changed from target-oriented procedures towards accurate mass full-scan MS techniques. This article describes these developments and addresses the implications of 2002/657/EC.
Latest:
UHPLC Coupled with Fourier Transform Orbitrap for Residue AnalysisThe trend in residue analysis has changed from target-oriented procedures towards accurate mass full-scan MS techniques. This article describes these developments and addresses the implications of 2002/657/EC.
Latest:
Determination of Methacholine Chloride and Potential Impurities Using a Reagent-free Ion Chromatography SystemMethacholine chloride is a synthetic analogue of the neurotransmitter acetylcholine used to assess bronchial asthma of subjects in respiratory function labs and epidemiological field studies.
Latest:
The Unique Selectivity of π-Interactions for Solid-Phase ExtractionHypercrosslinked polystyrene-type (solid-phase extraction) SPE materials exhibit a unique ability to enter p-interactions with aromatic, heterocyclic and unsaturated compounds. This property permits selective extraction and pre-concentration of the above classes of species from non-polar media and fatty matrices. The principle has been exploited for developing analytical protocols to determine polar furan derivatives in mineral transformer oil, polyaromatic hydrocarbons (PAHs) in smoked fish and for the fractionation of polychlorinated aromatic compounds in environmental matrices.
Latest:
Data Handling and Validation in Automated Detection of Food Toxicants Using Full Scan GC–MS and LC–MSAn overview of current chromatography-based food toxicant screening is presented.
Latest:
A Review of Column Developments for Supercritical Fluid ChromatographyThe authors review the requirements for columns specifically designed and manufactured for SFC.
Latest:
LC–MS-MS-Based Strategies for the Targeted and Nontargeted Screening of Contaminants in Food, Environmental, and Forensic SamplesLiquid chromatography coupled to mass spectrometry (LC–MS) using electrospray ionization (ESI) is a powerful analytical tool for the analysis of a wide molecular weight range of polar, semivolatile, and thermally labile compounds.
Latest:
Investigating Solvent Purity Using Comprehensive Gas Chromatography: A Study of AcetonesInvestigating Solvent Purity Using Comprehensive Gas Chromatography: A Study of Acetones
Latest:
Conquering Compliance: Robust Testing Solutions for Metals & PFAS in WaterWebinar Date/Time: Tue, Oct 8, 2024 10:00 AM EDT (4:00 PM CET)
Latest:
A Comparative Study on the Purification of Library Compounds in Drug Discovery Using Mass-Directed Preparative SFC and Preparative RPLCIn the past decade, supercritical fluid chromatography (SFC) has experienced a steady growth in acceptance, particularly in pharmaceutical and chemical laboratories. In SFC, "supercritical" CO2 combined with one or more polar organic solvents, most commonly alcohols, are used as mobile phase. Preparative SFC is deemed by many to hold the greatest promise to attain mainstream acceptance. The reduction in solvent consumption and collection in relatively small volumes of volatile organic solvents, hence, a much less stringent post-purification endeavour, lead to significant savings on operation costs. For example, Ripka et al. calculated that 20000 samples purified by SFC instead of reverse phase liquid chromatography (RPLC) would realize a 48 times reduction in solvent consumption.1
Latest:
Rapid LC Determination of UV Filters in Cosmetics using Ethanol as the Mobile PhaseA rapid and environmentally friendly LC method for the simultaneous determination of 12 UV filters in cosmetic samples using ethanol as the mobile phase.
Latest:
Resistive Glass Inlet Tubes Increase Ion ThroughputResistive glass tubes and plates are designed to guide ions by generating a uniform electric field. Photonis resistive glass products are composed of a proprietary lead silicate glass that has been specially processed to create a resistive layer at the surface. The resistivity can be varied over several orders of magnitude to suit the specific application.
Latest:
Fast Screening and Quantification of Pesticides Using an LC-MS Solution – The Pesticides Explorer CollectionA fully tested LC-MS/MS workflow for rapid and robust quantification of more than 250 pesticides below maximum residue limits (MRLs) with sensitivity, accuracy, and precision that meets stringent EU guidelines.
Latest:
Improving the Analytical Workflow for Protein Biopharmaceutical Characterization with a Novel LC–MS System SolutionThe ability to characterize protein therapeutics throughout the product development cycle is discussed.
Latest:
30-s UHPLC Separation of Pharmaceutical Ingredients using PDA DetectionUltra fast separations of acetaminophen, caffeine, propofol and parabens are achieved in 30 s using the Thermo Scientific Accela UHPLC system with the 80 Hz PDA detector. The performance of the Accela PDA detector is highlighted by its low noise level, low drift, four orders of magnitude linear working range and high sensitivity.
Latest:
Analysis of Polymers and Protein Nanoparticles using Asymmetrical Flow Field-Flow Fractionation (AF4)This article describes some of the latest developments for the analysis of polymers and nanoparticles.
Latest:
The Benefits of a Liquid-Electron Ionization Liquid Chromatography–Mass Spectrometry InterfaceThis is the fourth in a series of articles exploring current topics in separation science that will be addressed at the HPLC 2019 conference in Milan, Italy, from 16–20 June.
Latest:
Enhancing Phosphotyrosine Proteome Coverage Using a Combined ETD and CID Approach on a LTQ Orbitrap XL ETDCollision-induced dissociation (CID) and electron transfer dissociation (ETD) are complementary mass spectrometric fragmentation techniques. We have used CID and ETD in different approaches to analyse tyrosine phosphorylation using a Thermo Scientific LTQ Orbitrap XL equipped with ETD.
Latest:
How To Buy Gas Calibration MixesIn January 2004, LCGC Europe published an article on methods for selecting pure gases for analytical applications. 1 Here, the authors expand on this topic by addressing questions regarding gas mixtures - defined simply as more than one gas in a cylinder.
Latest:
Rapid Analysis of Pesticides in Difficult Matrices Using GC–MS–MSPesticides are widely used in agriculture to protect crops and to improve efficiency of production. Consequently, governments, food producers and food retailers have a duty to ensure that any residues occurring in foods for human consumption are at or below statutory maximum residue levels (MRLs). Regulation EC 396/2005 adopted in the European Union sets MRLs for more than 500 different pesticides in over 300 different food commodities.
Latest:
GCxGC-FID for Qualitative and Quantitative Analysis of PerfumesExploring the use of GCxGC-FID as a technique for qualitative and quantitative analysis of perfumes
Latest:
Separation of Quaternary Ammonium Compounds Using a Bonded Polymeric Zwitterionic Stationary PhaseUsing a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry. The impact of ionic strength and organic content in the mobile phase is discussed.
