Author
Latest:
Dried Blood Spots and High-Resolution Mass Spectrometry for Discovery Fast PK BioanalysisThis article introduces the advantages of accurate mass high-resolution mass spectrometry LC–MS (HRMS) coupled to the dried blood spot (DBS) technique for fast PK applications in a discovery environment. Compared with the established norm of plasma bioanalysis using triple quadrupoles, HRMS coupled to DBS is a viable alternative. The benefit is access to critical new information (HRMS bioanalysis) and significantly less stress on the animal (DBS), both factors that potentially improve the quality of early PK data.
Latest:
Using LC–oa-TOF MSE with a Multivariate Statistical Sample Statistical Sample Profiling Strategy to Distinguish Chinese Red Ginseng from Korean Red GinsengBoth Chinese ginseng and Korean ginseng are similar plant species and undergo similar handling procedures when harvested and processed for sale. Despite their similarities, Korean ginseng commands a higher price than Chinese ginseng on the open market and is believed to produce different clinical effects than Chinese ginseng. Chinese researchers are now employing new techniques on the two varieties of ginseng to understand their chemical differences. HPLC/UV-based strategies for distinguishing the two types of ginseng have proven to be mostly ineffective due to lack of resolution. Using UltraPerformance liquid chromatography/orthogonal acceleration (oa)–TOF mass spectrometry and exact mass measurement, the authors developed a high-resolution method using multivariate statistical analysis for separating and identifying differences between Chinese ginseng and Korean ginseng at the molecular level.
Latest:
Asymmetrical Flow FFF as an Analytical Tool for the Investigation of the Physical Stability of Virus-Like ParticlesThe rational development of AF4 methods for the investigation of VLP is illustrated.
Latest:
Tips & Tricks GPC/SEC: How GPC/SEC Can Help to Reduce PET Plastic WasteOne of the major problems with plastics is recycling. Only a few materials can be recycled and the acceptance of recyclates is sometimes low. GPC/SEC can be applied to investigate the quality of materials containing recycled portions.
Latest:
Simultaneous Headspace-GC–FID Analysis for Methanol and Ethanol in Blood, Saliva, and Urine: Validation of Method and Comparison of SpecimensAn HS-GC-FID method for the determination of ethanol and methanol content in biological fluids is presented.
Latest:
A Generic Headspace GC Method for Residual Solvents in Pharmaceutical: Benefits, Rationale, and Adaptations for New Chemical EntitiesA generic static headspace gas chromatographic (HSGC) method for determining common residual solvents in pharmaceuticals is described.
Latest:
Identifying and Rectifying the Misuse of Retention Indices in Gas ChromatographyLCGC International spoke to Phil Marriott and Humberto Bizzo about a recent paper they published identifying the incorrect use of retention indices in gas chromatography and how this problem can be rectified in practice.
Latest:
A Generic Headspace GC Method for Residual Solvents in Pharmaceutical: Benefits, Rationale, and Adaptations for New Chemical EntitiesA generic static headspace gas chromatographic (HSGC) method for determining common residual solvents in pharmaceuticals is described.
Latest:
Comparison of Ion Trap and Q-TOF Mass Spectrometers for Protein IdentificationThis study uses the challenging analysis of a complex, multi-organ peptide digest of Caenorhabditis elegans (C. elegans) to compare the performance of a novel linear ion trap mass spectrometer and a quadrupole time-of-flight (Q-TOF) mass spectrometer.
Latest:
Chiral Purification with Stacked Injections and Collections Using the Prep 100 SFC MS Directed SystemChiral chromatography has become the preferred tool for enantiomer separations in the early stages of pharmaceutical development for the purpose of accurately identifying single pure enantiomers with pharmacologic, toxicological, and clinical information, as stipulated by the FDA.1
Latest:
Online Identification of Flavones from Flos Chrysanthemi by LC–MS-IT-TOFThe use of medicinal herbs as alternative treatment methods continues to grow. With this escalating use has come an increasing interest in determining the chemical compositions of these herbs in order to obtain a better understanding of their makeup and effects. In this study, Flos Chrysanthemi, a commonly used traditional Chinese medicine that has been cultivated for centuries, was analyzed to identify the main flavone compositions in one original breed of Flos Chrysanthemi (Hangbaiju) in China.
Latest:
Using Novel TOF-MS to Increase Sensitivity and Confidently Detect Drugs of Abuse in UrineThe analysis of urine for drugs of abuse via chromatographic methods is commonplace but can be complicated by high matrix effects and frequent coelution. Novel time-of-flight mass spectrometry in combination with sophisticated deconvolution software was tested and found to provide increased confidence in results due to the high sensitivity and quality of spectra achieved.
Latest:
Adjustment to the Chromatographic System in U.S. Pharmacopeia Monographs: Flexibility and LimitationsTo meet system suitability requirements, chromatographers might need to make adjustments to the high performance liquid chromatography (HPLC) operating conditions.
Latest:
Simultaneous Headspace-GC–FID Analysis for Methanol and Ethanol in Blood, Saliva and Urine: Validation of Method and Comparison of SpecimensA simultaneous headspace-GC–FID method was validated to establish methanol and ethanol assessment in blood, urine and saliva.
Latest:
ADME/Pharmacokinetic Studies from Serum and Plasma: Improvements in Sample Preparation and LC–MS Analysis of Therapeutic OligonucleotidesThe paramount problem in performing absorption, distribution, metabolism, and excretion (ADME)/pharmacokinetic studies of therapeutic oligonucleotides revolves around inefficient and labor-intensive sample preparation. These traditional methods require multiple steps - liquid–liquid extraction (LLE) and solid-phase extraction (SPE) - to extract therapeutic oligonucleotides from serum and plasma for liquid chromatography–mass spectrometry (LC–MS) analysis and thus are not practical for clinical studies with large numbers of samples. Furthermore, these methods tend to yield low recoveries and poor reproducibility. This article presents a revolutionary new method for performing sample cleanup of therapeutic oligonucleotides from serum and plasma. The method extracts many types of therapeutic oligonucleotides from biological matrices in a rapid four-step SPE protocol that eliminates the need for LLE and can be automated for large sample sets. In the testing presented, different..
Latest:
Trace-Level Aliphatic Amines in Cationic DrugsThe analytical challenge treated in the present work consists in determining sub-ppb concentrations of low-molecular-weight amines in the presence of strongly retained cationic drugs by using ion chromatography (IC) with upstream in-line coupled-column matrix elimination (CCME).
Latest:
Rapid Quality Control of Flavours and Fragrances using Fast GC–MS and Multi-MS Library Search ProceduresUsing narrow-bore column fast GC combined with rapid scanning qMS to analyse flavours and fragrances is described.
Latest:
Phosphorylation Site Localization Using Probability-Based ScoringThe development of a phosphorylation probability scoring tool in an automated data search engine resolves ambiguity in site localization when compared to manual methods.
Latest:
Buffer Considerations for LC and LC–MSThe authors examine some critical factors with regard to buffers in LC and LC-MS research.
Latest:
Popular Cell Expression Systems in the Biotechnology IndustryThe authors describe the most common cell-based protein expression systems and purification strategies used in the biotechnology industry.
Latest:
Racing Against Time: High-Throughput Analysis of Dermorphin in Equine UrineThis article examines the problem of dermorphin doping in horse racing and presents an effective method for its detection using LC–MS TQ.
Latest:
Light Scattering for the Masses Monoclonal Antibodies and the Preparative Use of Flow Field-Flow Fractionation (AF4)Proteins - especially monoclonal antibodies (MABs) - have become increasingly important in pharmaceutical work. However, there are some important differences between conventional, chemically-synthesized drugs and proteins. Because of the complex and weak structure of proteins, even a slight change in conditions, such as pH value, temperature, or mechanical stress, may lead to aggregation and a loss of activity or stability.
Latest:
Popular Cell Expression Systems in the Biotechnology IndustryThe authors describe the most common cell-based protein expression systems and purification strategies used in the biotechnology industry.
Latest:
Improving the Analytical Workflow for Protein Biopharmaceutical Characterization with a Novel LC–MS System SolutionThe ability to characterize protein therapeutics throughout the product development cycle is discussed.
Latest:
Quantitative Analysis of Carbonyl-DNPH Derivatives by UHPLC–UVThermo Fisher Scientific Application Note
Latest:
Scaling LC Methods Using Porous Particle Stationary PhasesThe use of ultrahigh-pressure liquid chromatography (UHPLC) is now commonplace among pharmaceutical laboratories. However, until depreciation cycles replace traditional high performance liquid chromatography (HPLC) systems that operate at a maximum pressure of 400 bar, the advantages of UHPLC cannot be realized worldwide. Thus, product methods developed using UHPLC capabilities cannot directly transfer these methods to receiving laboratories without qualified UHPLC availability.
Latest:
Bioanalytical Analysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC–MS, Part 2: Sample PreparationThis concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.
Latest:
Determining Organotin Compounds in Marine Water and Sediment Samples using HS-SPME–GC–FIDThis article describes a fast, simple and clean procedure to determine three organotin compounds (monobutyltin, dibutyltyin and tributyltin) in environmental samples.
Latest:
Advances in the Analysis of Persistent Halogenated Organic CompoundsThis article reviews advances in the analysis of persistent halogenated organic compounds over the last century.
Latest:
Hyphenated Electronic Nose Technique for Aroma Analysis of Foods and BeveragesMass spectrometry-based electronic nose technology (MS-nose technology) is a fast hyphenated technique for digital odour characterization of food and beverage products.
