Author


Wim Witdouck

Latest:

Development of a Miniature Gas Chromatograph (µCAD) with Sample Enrichment, Programmed Temperature GC and Plasma Emission Detection (PED)

A miniature gas chromatograph incorporating a miniaturized chemical trap for enrichment, rapid thermal desorption of the trap, a resistively heated capillary column for programmed GC analysis and a micro-chip-based plasma emission detector (PED) is described. The sampling and chromatographic conditions for the analysis of volatile compounds in air are presented. The performance of the µCAD is illustrated in the universal (carbon) mode and for the selective detection of chlorinated and organo-mercury compounds. Detection limits (DLs) are at the sub-μg/L level in the carbon mode and 10 ng/L for organo-mercury compounds.


Tong Zhang

Latest:

3 μm Particle-Based Chiral Stationary Phases for the Fast and Efficient Resolution of Enantiomers

The move from conventional particle sizes (5 μm or higher) to smaller diameter packing materials is one of the most attractive approaches to achieve higher separating efficiency. Recently developed 3 μm polysaccharide-derived chiral stationary phases demonstrate characteristics of favourable mass transfer kinetics, high column efficiency and good column permeability. This allows the fast analysis of enantiomers using conventional HPLC instruments.


Keith Worral

Latest:

Advances in TOF-MS-Based Screening for Food Safety Residue Analysis with a Positive Approach

Mass spectrometry plays an increasingly significant role in the analysis of residues and contaminants in food. Here we will illustrate how the combination of ultrahigh-pressure liquid chromatography (UHPLC) and high-resolution time-of-flight-mass spectrometry (TOF-MS) is used to generate a screen of veterinary drug residues in products of animal origin. The use of UHPLC–TOF-MS and dedicated, workflow directed software allows rapid screening for large numbers of residues and automated quantification of positive samples. In addition, we illustrate how the data generated using MSE acquisition mode enable critical structural information to be collected, which offers additional selectivity and confirmatory data for compound identification and facilitates elucidation of the structure of newly discovered compounds.


Nick Bukowski

Latest:

A New Outlook on Soft Ionization for GC–MS

Despite the advantages of soft ionization ion-source technologies for improving confidence in the identification of a range of challenging analytes, soft ionization remains a niche technique for gas chromatography–mass spectrometry (GC–MS).


Hang Thu Ta

Latest:

Light Scattering for the Masses Effect of Heating on Molecular Weights of Chitosan

Heating is a common and essential requirement in most biopharmaceutical production methods. Chitosan is an amino polysaccharide obtained by alkaline deacetylation of naturally abundant chitin.


Thorsten Hofe

Latest:

Tips & Tricks GPC/SEC: Column Issues with Light Scattering Detectors

The addition of a light scattering detector dramatically increases the capabilities of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) analysis. However, the complexity of the system also increases. This instalment of Tips & Tricks discusses column issues when working with light scattering detectors.


Leonard M. Sidisky

Latest:

Chiral Capillary Gas Chromatography: A Highly Selective Analytical Tool

What can chiral capillary GC do for you? The answer lies in what analysis one is attempting to accomplish. Here, we discuss the utility of chiral capillary GC and where the technique is most valuable, focusing on three application areas in particular.


Arjen Gerssen

Latest:

Advances in Micro-Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry in Residue and Contaminant Analysis

Advances in nano-ultrahigh-performance liquid chromatography–mass spectrometry (nUHPLC) and micro–UHPLC (?UHPLC) are described and evaluated, with reference to the analysis of veterinary drugs and steroids in porcine meat and urine, respectively.


James S. Chang

Latest:

Nontargeted Screening and Accurate Mass Confirmation of Pesticides Using High-Resolution LC–Orbital Trap Mass Spectrometry

The increasing use of pesticide testing coupled with reductions in maximum permissible residue levels of pesticides in food have driven demand for fast, sensitive, and cost-effective analytical methods for high-throughput screening of multiclass pesticides in food. Detection of 510 pesticides at low parts-per-billion levels can be achieved within minutes using orbital trap technology. The high resolving power of these systems enables accurate mass confirmation of all compounds, including isobaric pesticides. This article will provide an overview of current legislation and illustrate how mass spectrometry instrumentation can enable fast and accurate pesticide screening.


Harold McNair

Latest:

A History of Gas Chromatography: My Early Experiences

Industry veteran and 2009 LCGC Lifetime Achievement Award Winner Harold McNair writes about the early days of gas chromatography and his experiences through the years.


Naoto Furasawa

Latest:

Rapid Sample Preparation Followed by a 100% Water Mobile Phase HPLC Analysis for Quantifying Acetamiprid and its N-desmethyl Metabolite, IM-2-1, in Cow’s Milk

This article describes a space-saving, quick, and inexpensive sample preparation technique followed by a high performance liquid chromatography (HPLC) method with a 100% water mobile phase and photodiode array (PDA) detection for quantifying acetamiprid and its N-desmethyl metabolite, IM-2-1, in cow’s milk. The analytes were extracted from the sample and deproteinized using a handheld ultrasonic homogenizer with 5% (w/v) trichloroacetic acid solution, purified using a centrifugal monolithic silica spin minicolumn, and quantified within 20 min per sample. The accuracy and precision are well within the international method acceptance criteria.


Tharanga Payagala

Latest:

The Advent and Potential Impact of Ionic Liquid Stationary Phases in GC and GCxGC

This article discusses the unique properties of ILs and their potential impact as GC stationary phases.


Paul D. Rainville

Latest:

LCGC NA - The Quantification of Clopidogrel in Human Plasma

LCGC NA - The Quantification of Clopidogrel in Human Plasma


Filip Van Opstaele

Latest:

Real-Time Profiling of Volatile Malt Aldehydes Using Selected Ion Flow Tube Mass Spectrometry

The authors use headspace SIFT-MS to target and identify volatiles in various malt aldehydes. The specificity and speed are compared to current methodology.



Silvia Talamentes

Latest:

Rapid LC Determination of UV Filters in Cosmetics using Ethanol as the Mobile Phase

A rapid and environmentally friendly LC method for the simultaneous determination of 12 UV filters in cosmetic samples using ethanol as the mobile phase.


Yıldırım Doğan

Latest:

Therapeutic Diazepam Monitoring in Human Plasma and Urine by HPLC: An Application for Alcoholism

A rapid and simple high performance liquid chromatography (HPLC) method with basic extraction assays was developed to investigate free diazepam levels in the plasma and urine samples of patients medicated with this drug for the management of alcohol withdrawal syndrome. The HPLC analysis was optimized and evaluated for linearity, imprecision, recovery, detection and quantification limits. The method showed linearity between 50–500 ng/mL (r2 ≥ 0.990). Coefficients of variations (%CV) were calculated to be in the range of 1.77–9.60. According to ICH guidelines, theoretical limits of detection (LOD) and quantification (LOQ) for plasma and urine were calculated as 8.3 ng/mL, 27.5 ng/mL and 8.2 ng/mL, 26 ng/mL respectively. Diazepam monitoring in plasma and urine displayed remarkable variations. The importance of adjusting doses according to individual requirements and the routine monitoring of plasma or urine for patients under medication is highlighted.


Glenn Cudiamat

Latest:

Flash Chromatography

Flash chromatography is a purification technique that is designed for rapid separation by using air pressure as opposed to slow and inefficient gravityfed chromatography. It differs from the conventional column technique by using slightly smaller silica gel particles and pressurized gas at 50–200 psi.



Damian Morrison

Latest:

Basic Principles for Purification Using Supercritical Fluid Chromatography

Although supercritical fluid chromatography (SFC) is not a new technique, preparative SFC is becoming increasingly more popular with advances in instrumentation, software and chemistry.


Milton L. Lee

Latest:

Exploring Biopharmaceutical Analysis with Compact Capillary Liquid Chromatography Instrumentation

A recent trend in the design of LC instrumentation is the move towards miniaturized and portable systems.


T. Moehring

Latest:

Enhancing Phosphotyrosine Proteome Coverage Using a Combined ETD and CID Approach on a LTQ Orbitrap XL ETD

Collision-induced dissociation (CID) and electron transfer dissociation (ETD) are complementary mass spectrometric fragmentation techniques. We have used CID and ETD in different approaches to analyse tyrosine phosphorylation using a Thermo Scientific LTQ Orbitrap XL equipped with ETD.



Kolbjorn Zahlsen

Latest:

A High-Volume, High-Throughput LC–MS Therapeutic Drug Monitoring System

LC-MS monitoring of the drug clozapine is detailed along with a description of the overall system architecture, workflow, and maintenance routines that spport a large-scale drug monitoring program.


Judy Berry

Latest:

High Resolution UHPLC Analysis of Naringin in Grapefruit Juice

Grapefruit juice was analysed by gradient UHPLC for naringin - a major flavanoid in grapefruit juice that gives it its bitter taste. A ZORBAX Rapid resolution HD (RRHD) StableBond-C18 2.1 ? 150 mm, 1.8 ?m column was able to resolve over 60 peaks, including naringin in 7 minutes, and was re-equilibrated in just three minutes by operating at a high flow rate (0.8 mL/min). The high pressure generated (965 bar) is well within the 1200 bar operating range of the RRHD column and 1290 Infinity LC.


Karen A. MacPherson

Latest:

Advances in the Analysis of Persistent Halogenated Organic Compounds

This article reviews advances in the analysis of persistent halogenated organic compounds over the last century.


Marcel Kneepkens

Latest:

Unravelling the Complexity of Fragrances in HPC* Products Using TruTOF GC–TOF-MS with Automated Data Deconvolution

The composition and analysis of fragrance components in home and personal care (HPC) products is very complex and unquestionably time consuming.


Kate Yu

Latest:

Nontargeted Metabolite Profiling in Next-Generation Plant Breeding: A Case Study in Malting Barley

Non-targeted metabolite profiling by ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) is a powerful technique to investigate the influence of genetic and environmental influence on metabolic phenotype in plants. The approach offers an unbiased and in-depth analysis that can reveal molecular markers of desirable phenotypic traits which can be complementary to genetic markers in plant breeding efforts. Here, the power of non-targeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications.


Alan Millar

Latest:

Comparison of Extracts from Dry and Alcohol-Steamed Root of Polygonatum kingianum (Huang Jing) by Sub-2-µm–LC–TOF-MS

Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.


Larry T. Taylor

Latest:

Highlights of SFC

The SFC conference tackled theory, economics, scale-up, and more.