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Gunpowder Profiling for Brand Identification using HPLC–MSConclusively determine the make and manufacturer of gunpowders by characterizing their distinguishing ingredients.
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Magnet or Cell? A Comparison of High-Resolution Sector Field ICP-MS and Collision–Reaction Cell Quadrupole ICP-MSInductively coupled plasma–mass spectrometry (ICP-MS) is a mature method that offers reliable measurements across almost the entire periodic table. It has been established as the key methodology for investigating elemental concentrations, which play a central role in biological, environmental, chemical, and industrial processes. ICP-MS is capable of performing multielemental analyses in a single analytical run, achieving an overall productivity of more than 50 samples/h. The method also delivers lower detection limits compared to graphite furnace atomic absorption (GFAA) or inductively coupled plasma–optical emission spectrometry (ICP-OES).
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Improving HPLC Separation of PolyphenolsThis article highlights a number of recent developments in HPLC for polyphenol analysis.
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High Temperature GPC Analysis of Polyolefins with Infrared DetectionThe analysis of the molar mass distribution of polyethylene and polypropylene resins by GPC/SEC has always been considered a demanding task because of the requirement of high temperature operation for dissolution and complex hardware design, which often results in high maintenance cost, in particular related to the autosampler/injector and detector units, and in other problematic and consuming tasks such as solvent handling added to column fragility, sample degradation or detector sensitivity–stability.
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Analysis of Glyphosate, Glufosinate, and Metabolites (Oct 2024)An extraction method with an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 HPLC column.
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Fast Determination of Anthocyanins to Authenticate Pomegranate JuiceAnthocyanins are a sub-class of naturally electron deficient and powerful antioxidants called flavonoids that are responsible for the red, orange, and blue coloration in fruits and flowers.
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The Minnesota Chromatography Forum's 30th AnniversaryPeter Johnson gives readers the history of the well-known Minnesota Chromatography Forum.
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Rapid Method Development for Industrial LC Separations Using Automated Screening of Stationary Phases and SolventsRapid Method Development for Industrial LC Separations Using Automated Screening of Stationary Phases and Solvents.
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Improving HPLC Separation of PolyphenolsThis article highlights a number of recent developments in HPLC for polyphenol analysis.
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Qualitative and Quantitative Metabolite Identification for Verapamil in Rat Plasma by Sub-2-μm LC Coupled with Quadrupole TOF-MSAn effective metabolite identification study should ideally include both qualitative and quantitative information that for both identifying metabolites, and determining the rate of clearance and the metabolic routes of the parent drug. Liquid chromatography–mass spectrometry (LC–MS) is considered the standard analytical technique for metabolite identification studies. To date, however, qualitative and quantitative information has always been obtained from two separation platforms: quadrupole time-of-flight (QTof) MS for the exact mass full-scan qualitative study, and tandem quadrupole MS for the multiple reaction monitoring (MRM) quantitative study. With advancements to QTof instrumentation, specifically, recent improvements in sensitivity and dynamic range, it is now possible to perform both qualitative and quantitative experiments on a single QTof mass spectrometer. This article describes a workflow that allows simultaneous qualitative and quantitative metabolite identification studies to be..
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Determining Organotin Compounds in Marine Water and Sediment Samples using HS-SPME–GC–FIDThis article describes a fast, simple and clean procedure to determine three organotin compounds (monobutyltin, dibutyltyin and tributyltin) in environmental samples.
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Using LC–oa-TOF MSE with a Multivariate Statistical Sample Statistical Sample Profiling Strategy to Distinguish Chinese Red Ginseng from Korean Red GinsengBoth Chinese ginseng and Korean ginseng are similar plant species and undergo similar handling procedures when harvested and processed for sale. Despite their similarities, Korean ginseng commands a higher price than Chinese ginseng on the open market and is believed to produce different clinical effects than Chinese ginseng. Chinese researchers are now employing new techniques on the two varieties of ginseng to understand their chemical differences. HPLC/UV-based strategies for distinguishing the two types of ginseng have proven to be mostly ineffective due to lack of resolution. Using UltraPerformance liquid chromatography/orthogonal acceleration (oa)–TOF mass spectrometry and exact mass measurement, the authors developed a high-resolution method using multivariate statistical analysis for separating and identifying differences between Chinese ginseng and Korean ginseng at the molecular level.
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The Use of Hollow Fibers in Liquid-Phase MicroextractionIn this installment, the subject of LPME is reviewed, with emphasis on the use of hollow fibers.
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Per- and Polyfluorinated Alkylsubstances (PFAS) from Dairy and Fish Using QuEChERS, SPE, and LC–MS/MSThe analysis of per- and polyfluorinated alkyl substances (PFAS) in food samples, along with an optional additional solid-phase extraction (SPE) clean-up for even more sensitive liquid chromatography tandem mass spectrometry (LC–MS/MS), is demonstrated in this article.
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Sampling Volatiles From Fragranced Consumer Products Using High-Capacity Sorptive ExtractionThis study describes the analysis of fragranced washing detergent and washing powder using probe-based headspace and immersive sorptive extraction, in conjunction with analysis by thermal desorption–gas chromatography–mass spectrometry (TD–GC–MS). As well as discussing the differences between the two samples, the analyte ranges covered by headspace and immersive sampling are compared.
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Gaining New Insights in Advanced Polymeric Materials Using Comprehensive Two-Dimensional Liquid ChromatographyTwo-dimensional LC, with advanced modulation techniques, can advance the analysis of advanced polymeric materials, assisting in characterizing copolymer composition heterogeneity and identifying ingredients in complex products.
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High Throughput Extraction of Melamine using EVOLUTE CX Mixed-mode SPE PlatesThe use of EVOLUTE CX mixed-mode columns provides high and consistent recoveries across a range of complex matrices, from biological fluids to milk products. The dual retention mechanism ensures that matrix components such as proteins, salts and phospholipids are removed, resulting in lower ion suppression and accurate analysis.
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Static Headspace GC–MS Detection of Residual Solvents—A Possible Simultaneous Identification and Quantitation MethodA new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
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HSS HPLC Columns: An Additional Option for Transferring Methods between HPLC and UPLC TechnologyAs UPLC users convert or replace their existing HPLC systems with UPLC systems there is a transition period where a method must be run on both platforms. Thus, having the same particle substrate and bonded phases available in HPLC and UPLC particle sizes can significantly ease the burden of method development and transfer from one platform to another. In addition to the ethylene bridged hybrid (BEH) particle, three new high strength silica (HSS) stationary phases for HPLC applications are introduced. Scalability between both column diameter and particle size is demonstrated on both UPLC and HPLC instrumentation.
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Advances in Sample Preparation for Food AnalysisSample preparation is a crucial part of the analysis of foodstuffs. Current sample preparation techniques used in food analysis are reviewed and the advantages and drawbacks of each one are discussed.
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Improved Analysis of FAMEs using Gas Chromatography with Vacuum Ultraviolet Detection (GC–VUV)The powerful characteristics of gas chromatography (GC) with VUV (vacuum ultraviolet) detection for the analysis of saturated and unsaturated fatty acids are described.
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Simultaneous Headspace-GC–FID Analysis for Methanol and Ethanol in Blood, Saliva and Urine: Validation of Method and Comparison of SpecimensA simultaneous headspace-GC–FID method was validated to establish methanol and ethanol assessment in blood, urine and saliva.
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Comparison of Liquid–Liquid Extraction (LLE) and Supported Liquid Extraction (SLE): Equivalent Limits of Quantification with Smaller Sample VolumesIn this application, we demonstrate the use of supported liquid extraction (SLE) for the extraction of beta blockers and NSAIDS from plasma compared with traditional liquid–liquid extraction. SLE was demonstrated to yield consistent LOQs using lower sample volumes.
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Selective Detection with UV Spectroscopy in Gas ChromatographyUV spectroscopy is an effective approach for tandem or parallel detection in gas chromatography, particularly in separations that require narrow bandwidths, such as GCxGC.
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Popular Cell Expression Systems in the Biotechnology IndustryThe authors describe the most common cell-based protein expression systems and purification strategies used in the biotechnology industry.
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Improving HPLC Separation of PolyphenolsPractical HPLC, HILIC and RP-LC methods of analysing common food and beverages containing polyphenols are presented.
Latest:
Using LC–oa-TOF MSE with a Multivariate Statistical Sample Statistical Sample Profiling Strategy to Distinguish Chinese Red Ginseng from Korean Red GinsengBoth Chinese ginseng and Korean ginseng are similar plant species and undergo similar handling procedures when harvested and processed for sale. Despite their similarities, Korean ginseng commands a higher price than Chinese ginseng on the open market and is believed to produce different clinical effects than Chinese ginseng. Chinese researchers are now employing new techniques on the two varieties of ginseng to understand their chemical differences. HPLC/UV-based strategies for distinguishing the two types of ginseng have proven to be mostly ineffective due to lack of resolution. Using UltraPerformance liquid chromatography/orthogonal acceleration (oa)–TOF mass spectrometry and exact mass measurement, the authors developed a high-resolution method using multivariate statistical analysis for separating and identifying differences between Chinese ginseng and Korean ginseng at the molecular level.
