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Bare Silica Has a Future in Supercritical Fluid ChromatographyThe authors compare five bonded phases with bare silica for the SFC separation of a 19-component mixture of polar analytes.
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Reliable and Efficient Sulfur Detection in Proteins Using ICP-MS with Capillary LCWith recent research, the University of Oviedo's analytical spectrometry research group has taken a step closer to the absolute quantification of proteins. Quantification based upon isotope dilution mass spectrometry of sulfur is hampered by gas-based polyatomic interferences. By implementing a quadrupole inductively coupled mass spectrometer with collision/reaction cell technology, the group has been able to overcome the issues and has increased reliability while optimizing the efficiency of its analyses.
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Ultra-Sensitive Method for the Detection of Illegal Dyes in Food SpicesSynthetic azo- and non-azo dyes were once commonly used as food colourings in many countries. Food safety regulators in Europe, the U.S, and other countries have now banned the use of these synthetic dyes in food because of their potential genotoxic and carcinogenic effects. In some countries, however, these dyes are still being used, especially in spices. There are currently no published legal limits for these illegal food dyes, but any detectable amount is deemed unacceptable. Thus, any analytical method used to test foods for these illegal dyes must be highly sensitive. Conventional methods are only able to provide limits of quantitation (LOQs) of 10–1000 ppb for these illegal food dyes. A reversed-phase ultrahigh-pressure liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) method has been developed that reliably achieves LOQs that are three-to-four orders of magnitude lower than conventional methods while also providing improved accuracy and reproducibility.
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Using LC–oa-TOF MSE with a Multivariate Statistical Sample Statistical Sample Profiling Strategy to Distinguish Chinese Red Ginseng from Korean Red GinsengBoth Chinese ginseng and Korean ginseng are similar plant species and undergo similar handling procedures when harvested and processed for sale. Despite their similarities, Korean ginseng commands a higher price than Chinese ginseng on the open market and is believed to produce different clinical effects than Chinese ginseng. Chinese researchers are now employing new techniques on the two varieties of ginseng to understand their chemical differences. HPLC/UV-based strategies for distinguishing the two types of ginseng have proven to be mostly ineffective due to lack of resolution. Using UltraPerformance liquid chromatography/orthogonal acceleration (oa)–TOF mass spectrometry and exact mass measurement, the authors developed a high-resolution method using multivariate statistical analysis for separating and identifying differences between Chinese ginseng and Korean ginseng at the molecular level.
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The Use of Subtle Differences in Selectivity to Improve Separation of Structurally Diverse AnalytesThe separation of structurally diverse analytes is often complicated by chance coelutions with other analytes or with matrix related compounds. Often the column is blamed, but while such coelutions make analysis difficult they do not necessarily indicate a faulty column, poor chromatography or method design.
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Kinetic Plots Made EasyGuest "LC Troubleshooting" columnist Uwe Neue unravels the mysteries surrounding kinetic plots to make them accessible to everyone
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Rapid Method Development for Industrial LC Separations Using Automated Screening of Stationary Phases and SolventsRapid Method Development for Industrial LC Separations Using Automated Screening of Stationary Phases and Solvents.
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Considerations on Switching from Helium to HydrogenThe authors explain the operational considerations for switching from helium to hydrogen in the separations lab, as well as discussing options.
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The QuEChERS Revolution!Ron Majors interviews the inventors of QuEChERS to explore the sucesses, challenges and future potential of this innovative sample preparation technique.
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Hyphenated Electronic Nose Technique for Aroma Analysis of Foods and BeveragesMass spectrometry-based electronic nose technology (MS-nose technology) is a fast hyphenated technique for digital odour characterization of food and beverage products.
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Dried Blood Spots and High-Resolution Mass Spectrometry for Discovery Fast PK BioanalysisThis article introduces the advantages of accurate mass high-resolution mass spectrometry LC–MS (HRMS) coupled to the dried blood spot (DBS) technique for fast PK applications in a discovery environment. Compared with the established norm of plasma bioanalysis using triple quadrupoles, HRMS coupled to DBS is a viable alternative. The benefit is access to critical new information (HRMS bioanalysis) and significantly less stress on the animal (DBS), both factors that potentially improve the quality of early PK data.
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Why and How to Avoid Ionic Contamination in Water Used for LC–MS AnalysesIonic contaminants in the water used in UHPLC analyses with MS detection method lead to adduct formation and reduced analytical signals because of ion suppression. In MS, the preferred ion type is the protonated molecular ion, especially in peptide analysis, since the partially mobile proton charge enables more meaningful fragmentation analysis, as compared to a sodiated peptide ion. Moreover, the occurrence of protonated analyte signals indicates that solvents and reagents, as well as the MS instrument used in analyses, are clean and do not contribute any contaminating cationic components to the analytical process. In the experiments presented here, it was observed that the signal intensities of the protonated species decreased as the sodium ion concentration in the water increased. This was accompanied by an increase in the intensity of sodiated adducts.
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Hyphenated Techniques as Modern Detection Systems in Ion ChromatographyThis article describes the use of combined ion chromatography-mass spectrometry (IC–MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP–MS) to analyze potentially harmful compounds.
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A Novel Sample Preperation Approach to Increase the Throughput of Pesticide Analysis by LC–MS–MSA fast clean and simple method to determine three organotin compounds in environmental samples is described
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A Novel Sample Preperation Approach to Increase the Throughput of Pesticide Analysis by LC–MS–MSA fast clean and simple method to determine three organotin compounds in environmental samples is described
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Advances in the Analysis of Persistent Halogenated Organic CompoundsThis article reviews advances in the analysis of persistent halogenated organic compounds over the last century.
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Using LC–oa-TOF MSE with a Multivariate Statistical Sample Statistical Sample Profiling Strategy to Distinguish Chinese Red Ginseng from Korean Red GinsengBoth Chinese ginseng and Korean ginseng are similar plant species and undergo similar handling procedures when harvested and processed for sale. Despite their similarities, Korean ginseng commands a higher price than Chinese ginseng on the open market and is believed to produce different clinical effects than Chinese ginseng. Chinese researchers are now employing new techniques on the two varieties of ginseng to understand their chemical differences. HPLC/UV-based strategies for distinguishing the two types of ginseng have proven to be mostly ineffective due to lack of resolution. Using UltraPerformance liquid chromatography/orthogonal acceleration (oa)–TOF mass spectrometry and exact mass measurement, the authors developed a high-resolution method using multivariate statistical analysis for separating and identifying differences between Chinese ginseng and Korean ginseng at the molecular level.
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Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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The Applicability of Field-Portable GC–MS for the Rapid Sampling and Measurement of High-Boiling-Point Semivolatile Organic Compounds in Environmental SamplesA look at the use of field-portable GC–MS with solid-phase microextraction, purge-and-trap, thermal desorption, and heated headspace sampling techniques to provide a fast response for in-field analysis of SVOCs in a wide variety of environmental-type samples including potable waters, tea, plants, and road gravel.
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Online Identification of Flavones from Flos Chrysanthemi by LC–MS-IT-TOFThe use of medicinal herbs as alternative treatment methods continues to grow. With this escalating use has come an increasing interest in determining the chemical compositions of these herbs in order to obtain a better understanding of their makeup and effects. In this study, Flos Chrysanthemi, a commonly used traditional Chinese medicine that has been cultivated for centuries, was analyzed to identify the main flavone compositions in one original breed of Flos Chrysanthemi (Hangbaiju) in China.
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Tracking Flavours in White Wine and Coffee Flavour Profiling of Food Samples with SPME-GC–MS and On-column InjectionSPME-GC–MS with on-column injection can be successfully employed for flavour profiling of food samples. The on-column injector of the GC-2010 can be used for SPME without any modifications, only an external ring adapter was used to facilitate the injection. The data can be compared with sensorial data of food.
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Asymmetrical Flow FFF as an Analytical Tool for the Investigation of the Physical Stability of Virus-Like ParticlesThe rational development of AF4 methods for the investigation of VLP is illustrated.
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Asymmetrical Flow FFF as an Analytical Tool for the Investigation of the Physical Stability of Virus-Like ParticlesThe rational development of AF4 methods for the investigation of VLP is illustrated.
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Determining Organotin Compounds in Marine Water and Sediment Samples using HS-SPME–GC–FIDThis article describes a fast, simple and clean procedure to determine three organotin compounds (monobutyltin, dibutyltyin and tributyltin) in environmental samples.
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Evaluation of PGC Stationary Phases Under High-Temperature LC Conditions for the Analysis of Parabens in Food SamplesThis article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.
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Analysis of Complex Pharmaceuticals by Ultrahigh-Pressure Liquid Chromatography: Case Studies and Quality Control ImplicationsExperimental results and method validation data from two analytical case studies of complex small?molecule pharmaceuticals illustrates the utility and advantages of UHPLC in high-resolution separations.
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Combating Terrorism with Mass Spectrometry — Screening People for ExplosivesIn this article, we review the capabilities and challenges of deploying mass spectrometry (MS) to homeland security screening requirements. The fidelity of MS for chemical analysis is well accepted; however, the effectiveness of an overall system in real-world work environments depends on solving big challenges in sample collection, speed of analysis, and ease-of-use, and maintaining continuous reliability. We compare MS to the more commonly used security method of ion mobility spectrometry (IMS) and illustrate the significant improvements that MS can provide. We then describe one of the first introductions of MS in homeland security, that being the screening of people for concealed explosives using a walk-through portal device.
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Increased Sensitivity, Improved Resolution and Faster Analysis Times For Compendial Methods: Determination of Impurities and Related Substances Using Newly Developed 2.6 µm Core-Shell Kinetex LC ColumnsRecently a newly developed Kinetex 2.6 µm core-shell chromatographic particle has been commercialized that offers the performance benefits of sub-2 µm fully-porous particles but at substantially lower operating pressures.
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Untargeted Screening and Identification of Contaminants Using Gas Chromatography Coupled to High-Resolution Accurate Mass–Mass Spectrometry: Novel Solutions for Applied Testing LaboratoriesLimitations often arise when using GC with quadrupole-based mass spectrometers for detecting volatile and semivolatile contaminants. Enter HRAMS-MS.
