Author
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Popular Cell Expression Systems in the Biotechnology IndustryThe authors describe the most common cell-based protein expression systems and purification strategies used in the biotechnology industry.
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Improving the Analytical Workflow for Protein Biopharmaceutical Characterization with a Novel LC–MS System SolutionThe ability to characterize protein therapeutics throughout the product development cycle is discussed.
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Quantitative Analysis of Carbonyl-DNPH Derivatives by UHPLC–UVThermo Fisher Scientific Application Note
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Scaling LC Methods Using Porous Particle Stationary PhasesThe use of ultrahigh-pressure liquid chromatography (UHPLC) is now commonplace among pharmaceutical laboratories. However, until depreciation cycles replace traditional high performance liquid chromatography (HPLC) systems that operate at a maximum pressure of 400 bar, the advantages of UHPLC cannot be realized worldwide. Thus, product methods developed using UHPLC capabilities cannot directly transfer these methods to receiving laboratories without qualified UHPLC availability.
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Bioanalytical Analysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC–MS, Part 2: Sample PreparationThis concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.
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Determining Organotin Compounds in Marine Water and Sediment Samples using HS-SPME–GC–FIDThis article describes a fast, simple and clean procedure to determine three organotin compounds (monobutyltin, dibutyltyin and tributyltin) in environmental samples.
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Advances in the Analysis of Persistent Halogenated Organic CompoundsThis article reviews advances in the analysis of persistent halogenated organic compounds over the last century.
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Hyphenated Electronic Nose Technique for Aroma Analysis of Foods and BeveragesMass spectrometry-based electronic nose technology (MS-nose technology) is a fast hyphenated technique for digital odour characterization of food and beverage products.
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Determination of Furan in Food by Gas Chromatography–Mass Spectrometry and Headspace SamplingPerkinElmer Application Note
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High Speed Separation of More Than 16 Polycyclic Aromatic HydrocarbonsPolyaromatic hydrocarbons (PAH) are particularly relevant in the analysis of environmental pollution because of their ubiquity, toxicity and persistence. Consequently, the PAHs have become the most intensively studied pollutants in environmental analysis.
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Comparison of Ion Trap and Q-TOF Mass Spectrometers for Protein IdentificationThis study uses the challenging analysis of a complex, multi-organ peptide digest of Caenorhabditis elegans (C. elegans) to compare the performance of a novel linear ion trap mass spectrometer and a quadrupole time-of-flight (Q-TOF) mass spectrometer.
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High Temperature GPC Analysis of Polyolefins with Infrared DetectionThe analysis of the molar mass distribution of polyethylene and polypropylene resins by GPC/SEC has always been considered a demanding task because of the requirement of high temperature operation for dissolution and complex hardware design, which often results in high maintenance cost, in particular related to the autosampler/injector and detector units, and in other problematic and consuming tasks such as solvent handling added to column fragility, sample degradation or detector sensitivity–stability.
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Trends and Challenges for Bioanalysis and Characterization of Small and Large Molecule DrugsStructural, bioanalytical, characterization, and quality control studies are critical for successful drug development. These studies must be as accurate, sensitive, and selective as possible, and liquid chromatography coupled to tandem mass spectrometry (LC–MS–MS) has been the technique of choice for many areas of small molecule analysis for the past 30 years. During that time, rapid improvements in analytical technologies have supported the development of more sensitive and robust methods. However, the pharma and biopharma industry continues to need more powerful instruments and more diverse methods, particularly as therapeutics have expanded to include large molecules. This work follows on from an earlier article that explored the limitations of LC–MS–MS for bioanalysis of biologics. This article considers some of the current issues for analysis of small and large molecules, and emerging trends in method development.
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Reducing Cycle Time for Analysis of 1,4-Dioxane Using an Automated Purge and Trap Sample Prep SystemWith advances in instrumentation and without regulatory method constraints, 1,4-dioxane can be detected at the part-per-billion (ppb) level, despite its poor purge efficiency. This application will manipulate purge and trap, as well as GC–MS parameters, to create a more efficient method to reduce cycle times. This study will utilize a Teledyne Tekmar Atomx Automated Sample Prep System in conjunction with an Agilent 7890/5975 GC–MS. A linear calibration and method detection limits (MDLs) for 1,4-dioxane will be established using this method.
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Fast Analysis of Charge Variants of Monoclonal Antibodies by Ion Exchange ChromatographyDevelopment and production of biopharmaceuticals is a growing segment of the pharmaceutical industry. The development of vaccines is only one - but as a result of the current H1N1 flu hysteria it is the most striking - segment of this emerging field. Recombinant proteins, ranging from insulin products to therapeutic antibodies, represent another important group of biopharmaceuticals. With the introduction of the first so-called biosimilars in Europe the demand for highly efficient analysis methods is further increasing. Newly developed stationary phases for the typical modes of biochromatography of native proteins such as SEC or IEC open up new opportunities for increasing throughput in the bioanalytical lab.
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Optimization of Asymmetrical Flow Field-Flow FractionationThis article illustrates how to choose the best experimental parameters for asymmetrical flow field-flow fractionation.
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Popular Cell Expression Systems in the Biotechnology IndustryThe authors describe the most common cell-based protein expression systems and purification strategies used in the biotechnology industry.
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An Improved SPE-LC–MS–MS Platform for the Simultaneous Quantification of Multiple Amyloid Peptides in Cerebrospinal Fluid for Preclinical or Biomarker DiscoveryFast, flexible platforms for peptide quantification are needed, particularly for a discovery setting. This type of methodology would be especially advantageous in the case of amyloid beta (a?) peptides.
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Hyphenated Electronic Nose Technique for Aroma Analysis of Foods and BeveragesMass spectrometry-based electronic nose technology (MS-nose technology) is a fast hyphenated technique for digital odour characterization of food and beverage products.
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Metabolite Profiling Applications in Early Drug DiscoveryMetabolite profiling in drug discovery can contribute significantly at the lead optimization stage in two main application areas. The first is the identification of major metabolites, which provides medicinal chemists with information on the metabolic "soft spots." These soft spots are locations on the molecule particularly susceptible to metabolic modification, which can contribute to high pharmacokinetic clearance. This information then can be used to optimize the structure of a lead compound or chemical series to slow the rate of metabolism and therefore reduce hepatic clearance. This improves the absorption, distribution, metabolism, and excretion (ADME) properties of the compound, such as bioavailability, exposure (as measured by area under the curve), and half-life. Through iterative optimization of the structure and timely generation of metabolism data following each structural modification, pharmacokinetic properties can be improved while maintaining activity against the therapeutic target.
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Reliable and Efficient Sulfur Detection in Proteins Using ICP-MS with Capillary LCWith recent research, the University of Oviedo's analytical spectrometry research group has taken a step closer to the absolute quantification of proteins. Quantification based upon isotope dilution mass spectrometry of sulfur is hampered by gas-based polyatomic interferences. By implementing a quadrupole inductively coupled mass spectrometer with collision/reaction cell technology, the group has been able to overcome the issues and has increased reliability while optimizing the efficiency of its analyses.
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Hydrogen as a Carrier Gas for GC and GC–MSHydrogen carrier gas can be an effective replacement for helium in many gas chromatography (GC) and GC–mass spectrometry (MS) applications.
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Fast Online SPE Purification of Stevia Plant ExtractsSample clean-up using solid-phase extraction (SPE) and similar methods can sometimes be both time-consuming and expensive. The purification of complex plant extracts requires special care in particular. A robust and sensitive online SPE sample preparation method is described for the determination of steviol glycosides.
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A Novel Sample Preperation Approach to Increase the Throughput of Pesticide Analysis by LC–MS–MSA fast clean and simple method to determine three organotin compounds in environmental samples is described
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Determining Organotin Compounds in Marine Water and Sediment Samples using HS-SPME–GC–FIDThis article describes a fast, simple and clean procedure to determine three organotin compounds (monobutyltin, dibutyltyin and tributyltin) in environmental samples.
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Online Identification of Flavones from Flos Chrysanthemi by LC–MS-IT-TOFThe use of medicinal herbs as alternative treatment methods continues to grow. With this escalating use has come an increasing interest in determining the chemical compositions of these herbs in order to obtain a better understanding of their makeup and effects. In this study, Flos Chrysanthemi, a commonly used traditional Chinese medicine that has been cultivated for centuries, was analyzed to identify the main flavone compositions in one original breed of Flos Chrysanthemi (Hangbaiju) in China.
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An Investigation into the Use of Alternate Carrier Gases for the Determination of Volatile Organic Compounds in Water by GC–MSThis study focuses on United States Environmental Protection Agency (US EPA) Method 524.3 for volatile organic compounds (VOCs) in water using gas chromatography–mass spectrometry (GC–MS).
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Simultaneous Headspace-GC–FID Analysis for Methanol and Ethanol in Blood, Saliva and Urine: Validation of Method and Comparison of SpecimensA simultaneous headspace-GC–FID method was validated to establish methanol and ethanol assessment in blood, urine and saliva.
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Developing a Fast Purification Method for a Natural Product with a Preparative LC Column Packed with Superficially Porous ParticlesSuperficially porous particles (SPPs) are routinely used in analytical liquid chromatography (LC) columns, but they also provide benefits for preparative LC columns for use in pharmaceutical discovery laboratories and high-throughput environments. In this study, a preparative method using SPP-packed column had a 45% decrease in run time over a method using columns packed with fully porous particles, at standard flow.
