Michael Koleto

A single calibration curve run with staggered calibrants bracketing the unknowns is compared to running complete duplicate calibration curves, one at the beginning and one at the end of unknown sample analysis in an effort to accelerate discovery bioanalysis.

This article introduces the advantages of accurate mass high-resolution mass spectrometry LC–MS (HRMS) coupled to the dried blood spot (DBS) technique for fast PK applications in a discovery environment. Compared with the established norm of plasma bioanalysis using triple quadrupoles, HRMS coupled to DBS is a viable alternative. The benefit is access to critical new information (HRMS bioanalysis) and significantly less stress on the animal (DBS), both factors that potentially improve the quality of early PK data.