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Analytical Advantages of Highly Stable Stationary Phases for Reversed-Phase LCDuring the past five years, many manufacturers of HPLC columns have focused on improving stationary phase stability and reproducibility. In this study, the authors use a variety of test solutes to compare the efficiency, selectivity, and hydrophobic retention mechanisms of five commercially available HPLC columns based silica, alumina, zirconia, and polystyrene cross-linked with divinylbenzene as the support.
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Metabolome Studies of Herbal Medicine Using High Performance Liquid Chromatography Ion-Mobility Mass SpectrometryIn Chinese herbal medicine (CHM), traditionally, Hedyotis diffusa and Scutellaria barbata are cooked together and given to patients as tea. It is hypothesized that the interaction of metabolites from both herbs during cooking, improves the medical effect. This hypothesis was tested by preparing two tea variants which were then analyzed using high performance liquid chromatography (HPLC) coupled to ion-mobility mass spectrometry (IM-MS).
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The Current Status and Future of Two- and Multidimensional Liquid Chromatography in Pharmaceutical R&D and QCThis article will discuss the benefits of 2D-LC and multiple application areas in (bio)pharmaceutical analysis, and will highlight the challenges and future outlook.
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A Flash Qualitative Identification Method for the Specific Component in a Mixture Based on Diode Array DetectorThis paper proposes a new method of flash qualitative identification (FQI) to qualitatively identify a certain target component from a mixture within half a second by disusing the analytical column, which is a time-consuming unit in current chromatography instruments. First, a Noised Spectrum Identification (NSI) model was constructed for the data set generated directly by diode array detector (DAD) without the process in an analytical column. Then, a method called vector error algorithm (VEA) was proposed to generate an error according to the DAD data set for a mixture and a specific spectrum for the target component to be identified. A criterion based on the error generated by the VEA is used to give a judgement of whether the specific spectrum exists in the DAD data set. Several simulations demonstrate the high performance of the FQI method, and an experiment for three known materials was carried out to validate the effectiveness of this method. The results show that the NSI model concurs with the real experiment result; therefore, the error generated by the VEA was an effective criterion to identify a specific component qualitatively, and the FQI method could finish the identification task within half a second.
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Characterization of Styrene–Butadiene Block Copolymers by Gel Permeation ChromatographyThe authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.
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Innovations In Sample Preparation: A Virtual Symposium*Live: Friday, June 18, 2021 at 1pm BST| 8am EDT* A special one-day symposium —chaired by Jared Anderson, a key opinion leader in the field of sample preparation and a member of the LCGC’s Editorial Advisory Board (EAB) — will highlight state-of-the art advances in sample preparation and will cover a wide range of topics *On Demand Until June 18, 2022*
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HPLC Detection and Evaluation of Chiral Compounds with a Laser-Based Chiroptical DetectorThe authors evaluate a new chiroptical detector's sensitivity for various chiral compounds, linear dynamic range, and relative response with different solvents.
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Simple Tips to Increase Sensitivity in U/HPLC AnalysisA main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.
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Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic FactorDisplacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.
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Multi-Active Method (MAM) for the Analysis of Agriculture Product Technical Ingredients and Formulated ProductsA collaborative multi-analyte method developed utilizes HPLC and UHPLC to analyze more than 70 active ingredients. This method offers a validated approach for determining technical AI alongside linearity, precision, accuracy, and specificity tests for seven active ingredients.
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Technical Note: Modifying a Standard HPLC Autosampler for On-Line Process MonitoringA simple modification of the standard HPLC autosampler flow path enables automated sampling and analysis of samples outside the autosampler unit when users cannot place the sample container inside because of size restrictions.
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Can Chemistry Be Accessible For Students With Visual Impairment?This article presents a multimodal approach for students with severe visual impairment or blindness, to encourage them to learn more about chromatographic processes.
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New Mass Spec Technique Provides Rapid Elemental Analysis of Battery MaterialsWebinar Date/Time: Tue, Sep 12, 2023 2:00 PM EDT
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Ion Chromatography Using Anion Exchangers Modified with Anionic PolysaccharidesThe authors demonstrate a new detection method for various anions and cations separated by ion chromatography and point out the main advantages of this method.
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Chromatographic Innovations for Cell TherapiesThe complexity and challenges of developing, manufacturing, and controlling cell therapies offer today’s chromatographic scientists new opportunities to join the journey of discovery and innovation needed to develop and commercialize this new drug modality. This article explores the latest developments and highlights the importance of new capillary electrophoresis–mass spectrometry (CE–MS)-based approaches to understand the efficiency of cell therapy production.
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In situ Hydrogenolysis with 3D-Printed Jet and Flame Ionization Detection for Carbon Oxides and Volatile Aldehydes AnalysisA new approach based on post-column in situ hydrogenolysis, and flame ionization was used here to improve characterization of volatile aldehydes. We review and describe that approach here.
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Ultrahigh-Pressure Liquid Chromatography Analysis of Passivators and Furanic Compounds in Insulating LiquidThis article introduces optimization of the two separate standard methods, BS148:2009 and ASTM D5837-15, to offer simultaneous analysis in one assay.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Merck KGaA, Darmstadt, Germany: Greener Sampling of Terpenes in CannabisThis application note demonstrates the use of Thermal Desorption as greener approach for sampling of terpenes in air for workplace exposure or terpene profiling
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Evaluation of PGC Stationary Phases Under High-Temperature LC Conditions for the Analysis of Parabens in Food SamplesThis article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.
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Millipore Sigma: BIOshell™ 1.5 mm for greener HPLCThis application notes showcases greener HPLC with less solvent using smaller inner diameter columns while maintaining the quality of separation
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Untargeted LC–MS Lipidomics with Data Independent Acquisition using Sequential Window Acquisition of All Theoretical Fragment-Ion SpectraA focus on untargeted UHPLC–MS/MS lipidomics with data independent acquisition using SWATH technology. This article explores the wide-ranging potential and peculiarities of SWATH–MS hyphenated with UHPLC for the profiling of lipidomes of various biological samples.
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Overcoming Incomplete Peptide Mapping of Antibody Complementarity-Determining Regions with Alternate Digestion WorkflowsPepsin digestion and guanidine hydrochloride post-digestion can improve sequence coverage in antibody peptide mapping compared with trypsin digestion.
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Multidimensional Gas Chromatography: Benefits and Considerations for Current and Prospective UsersWhen should multidimensional GC approaches be used for analyzing complex samples and analytes?
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Applying Sustainability Concepts to Modern Liquid ChromatographyGovernments are striving to implement policy changes towards a greater use of green technology, specifically around the generation of energy. Individuals and industrial organizations have also taken up the challenge, and now many companies are driving to significantly reduce their environmental footprint, or indeed become carbon negative within very short time frames.
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Detailed Glycosylation Analysis of Therapeutic Enzymes Using Comprehensive 2D-LC–MSIn this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.
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The Power of Trypsin Immobilized Enzyme Reactors (IMERs) Deployed in Online MDLC–MS ApplicationsCommercially available trypsin IMERs can digest proteins with high sequence coverage and robustness, facilitating online multidimensional LC–MS.
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A Study on the Optimization of the Ion-Pair Reversed Phase Liquid Chromatography Analysis of Protected And Unprotected DNA OligonucleotidesThis article describes a comprehensive study on optimizing ion-pair reversed-phase liquid chromatography for analysing protected and unprotected single-stranded DNA oligonucleotides.
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Combined Chromatographic and Spectrometric Approaches for Cleaning Verification of Small-Molecule Pharmaceutical Compounds in the Manufacturing EnvironmentWe assess recent advances in cleaning verification methods for small-molecule pharmaceuticals, and propose a workflow.
