Author
Latest:
A New Path to High-Resolution HPLC–TOF-MS — Survey, Targeted, and Trace Analysis Applications of TOF-MS in the Analysis of Complex Biochemical MatricesHigh performance time-of-flight mass spectrometry (TOF-MS) is applied to the analysis and characterization of complex biological samples. High mass accuracy, high resolution, and accurate relative isotope abundance are all applied to the determination of analytes covering a range of concentrations in complex matrices including plasma and urine. Qualitative and quantitative evaluations are provided and include demonstrations of the impact of high-performance MS on sensitivity and selectivity. The ability to leverage high-performance MS in conjunction with a broad dynamic range in rapid ultrahigh-pressure liquid chromatography (UHPLC) analyses to identify unknowns and to propose putative metabolic biomarkers is demonstrated. The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.
Latest:
Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification (Part 3): Performance Qualification (PQ)A look at the role of system suitability tests (SSTs) during performance qualification (PQ).
Latest:
Lab-on-a-Chip GC for Environmental ResearchThe development of a microfabricated comprehensive GC that can be used as a portable measurement device.
Latest:
25-Hydroxyvitamin D2/D3 Analysis in Human Plasma Using LC–MSOne of the biggest debates in clinical medicine currently revolves around understanding the proper levels of vitamin D in patients' plasma.
Latest:
Lab-on-a-Chip GC for Environmental ResearchThe development of a microfabricated comprehensive GC that can be used as a portable measurement device.
Latest:
LC Column Technology: The State of the ArtIn this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in liquid chromatography column technology, gas chromatography, sample preparation, and liquid chromatography instruments. They also describe the latest practical developments in supercritical fluid chromatography, 3D printing, capillary electrophoresis, data handling, comprehensive two‑dimensional liquid chromatography, and multidimensional gas chromatography.
Latest:
Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
Latest:
Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
Latest:
Insights into the Shape-Selective Retention Properties of Porous Graphitic Carbon Stationary Phases and Applicability for Polar CompoundsWhen the unique shape-selective properties of PGC are appropriately identified, PGC stationary phases can be harnessed for fine separations of structurally related compounds.
Latest:
Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable WatersA hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.
Latest:
New Method US EPA 625 with Solid Phase Extraction for Challenging WastewatersThis automated SPE methodology is a solution that will reduce analyst labor, solvent usage, and turn-around-time while maintaining the high quality results required within today's laboratories.
Latest:
Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
Latest:
Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
Latest:
Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
Latest:
Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
Latest:
Laser Diode Thermal Desorption Tandem Mass Spectrometry for Simultaneous Quantitation of Metformin and Sitagliptin in Mouse and Human Dried Blood SpotsAn exploration of LDTD-MS-MS and how it compares to HPLC–MS-MS techniques in terms of sensitivity, robustness, and speed in an in-vivo drug discovery application
Latest:
High-Throughput Quantitative Analysis of Vitamin D Using a Multiple Parallel LC–MS System Combined with Integrated On-Line SPEA newly developed high-throughput method for the quantitation of vitamin D using both multiplexed LC and on-line SPE is discussed.
Latest:
A Systematic Study of Achiral Stationary Phases Using Analytes with a Molecular Diversity ModelThe authors characterized 12 SFC achiral stationary phases with 60 compounds from four chemical classes to establish guidelines for th rational selection of the optimum stationary phase for separations.
Latest:
Food Metabolomics: Fact or Fiction?The different aspects of food metabolomics are described using tomato taste as an example.
Latest:
Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification (Part 3): Performance Qualification (PQ)A look at the role of system suitability tests (SSTs) during performance qualification (PQ).
Latest:
Online Hydrophobic Interaction Chromatography–Mass Spectrometry (HIC–MS) Analysis of ProteinsRecent advances in stationary phases for hydrophobic interaction chromatography (HIC) permit HIC–MS analysis of intact antibodies and other proteins using direct flow to the mass spectrometer.
Latest:
Novel C18 Stationary Phase Provides Exceptional Results for Metabolite ProfilingMetabolomics, the study of small molecule metabolites that are found within a biological sample, is an emerging field of study. Progress in this field depends upon technological advancement in the fields of LC–MS and separation technology.
Latest:
Separation Science: The State of the Art: New Frontiers in Multidimensional Liquid ChromatographyIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
Latest:
Time-Resolved SRM Analysis and Highly Multiplexed LC–MS-MS for Quantifying Tryptically Digested ProteinsThe inclusion of time-resolved selected reaction monitoring (SRM) functionalities into mass spectrometer control software allows large numbers of peptides to be quantified using short LC–MS-MS methods.
Latest:
Trace Metabolic Profiling and Pathway Analysis of Clomazone Using LC–MS-MS and High Resolution MSDetection, analysis, and characterization of low-abundant metabolites remain an unresolved problem in metabolic studies. In this study, we report a novel approach to address this challenge. The current methodology is derived from the predictive multiple reaction monitoring (pMRM) mode available on triple-quadrupole linear ion trap mass spectrometry (MS) systems. The pMRM mode offers the highest sensitivity among various acquisition modes for studying trace levels of metabolites of the herbicide clomazone in plants. Additionally, this method allows for the identification of positional isomers of metabolites.
