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Glycosylation in mAb Therapeutic Products: Analytical Characterization and Impact of ProcessGlycosylation of monoclonal antibody (mAb) therapeutics is widely recognized by the regulators and the industry as a critical quality attribute (CQA). Hence, it is necessary that glycosylation is measured and adequately controlled during production. This instalment reviews the various process parameters and raw material attributes that affect glycosylation, as well as the different analytical tools that are used for characterization, with greater emphasis on the chromatographic methods of analysis. Key recent advancements that have occurred in the past five years are also discussed briefly. While significant progress has been made in the monitoring of glycosylation, its real time control has yet to be demonstrated.
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Anomalous Retention Prediction Using Modelling Software in Gradient Reversed-Phase Liquid Chromatography: Why it Can Occur and How to Prevent ItThe success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
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Quantifying Small Molecules by Mass SpectrometryThe use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration.
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Quantitation of Albumin and Creatinine in Urine by MALDI-TOF Mass SpectrometryThis MS-based method represents a simple, fast, and attractive alternative to current immunoassay-based methods for the quantitation of albumin and creatinine in urine. This protocol enables the direct detection and measurement of the intact analytes from the same sample preparation, requiring only a 10-fold dilution of a urine sample into a MALDI-TOF matrix solution.
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Fast and Accurate Determination of Algal Toxins in Water Using On-line Preconcentration and UHPLC–HRAM–MSThe development of a column-switching technique based on on-line preconcentration and HRAM–MS to obtain fast and accurate results for the determination of algal toxins in drinking water is discussed.
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On-line Monitoring of Atmospheric Inorganic Gases and Aerosols in the Southeastern and Northwestern U.S.Metrohm International
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Screening of Drugs of Abuse Using the Velox 360 Paper Spray SystemPaper spray mass spectrometry (MS) allows for the direct analysis of pharmaceuticals, drugs of abuse, and other small molecules from blood, urine, and other biofluids (1,2).
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A Standardized 2D-LC Screening Platform for Peak Purity Determination in Pharmaceutical AnalysisThis study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.
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GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic CompoundsIn most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.
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Determination of Cannabinoid Content and Pesticide Residues in Cannabis Edibles and BeveragesAs a result of the rapid growth of the cannabis industry, many testing laboratories are looking for efficient, reliable, and cost-effective analytical methods to analyze chemical residues, such as pesticides, mycotoxins, solvent residues, terpenes, and heavy metals, as well as cannabinoid concentration in cannabis-infused edibles and beverages. In this article, QuEChERS (quick, easy, cheap, effective, rugged, and safe), a sample preparation technique widely adopted in the food testing industry, is introduced to the discipline of forensic testing as a viable method for the extraction of pesticides and cannabinoids in various complex sample matrices. The claimed amounts of cannabinoids versus the actual amounts are compared, as well as the pesticide residue levels in edible and beverage samples.
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Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
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The Effect of SEC Column Arrangement of Different Pore Sizes on Resolution and Molecular Weight MeasurementsThe results of a study to determine the influence of SEC column order on molecular size separation and peak broadening
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Optimizing Flavour Analysis Using Modern Heart-Cutting MDGCThis review discusses recent technological advances in classical heart-cuttting two-dimensional gas chromatography (GC–GC). These developments are then illustrated by application to analysis of important flavour compounds at trace levels in very complex matrices
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HPLC Systems "By the Zones"To understand how the HPLC system works, it can be useful to first identify the basic components of virtually every system. Armed with that information, envisioning the HPLC system as comprised of three different zones - each with unique characteristics and each with its own set of requirements - can offer further guidance.
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Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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A Single-Method Approach for the Analysis of Volatile and Semivolatile Organic Compounds in Air Using Thermal Desorption Coupled with GC–MSThis article describes a new, single method to replace the two-method approach using United States EPA Methods TO-15 and TO-13A for the analysis of both VOCs and SVOCs in air.
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Novel Methods Using Mass Spectrometry for Food Safety—From Contamination to NutritionIn the human food supply, public confidence is affected by contaminants and misreporting of nutritional information. This article highlights three events that required development of new mass spectrometry methods, including the detection of pesticides (such as fipronil and glyphosate), and the detection and quantification of fat-soluble vitamins.
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When Bad Things Happen to Good Food: Application of HPLC to Detect Food AdulterationIn this instalment, guest authors Jeff Hurst and Kendra Pfeifer from Hershey Foods explore high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), and mass spectrometry (MS) approaches being adopted to keep ahead of the food adulteration game.
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Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
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Application of an LC–MS/MS Library for the Identification and Quantitation of Pesticides in Food Safety Using MRM Spectrum ModeThis article describes the application of liquid chromatography tandem mass spectrometry (LC–MS/MS) and enhanced multiple reaction monitoring (MRM) spectrum libraries for multi-pesticide residue analysis. Using these methods, a high number of fragment ion transitions of target compounds can be examined to increase specificity and reporting confidence, reducing the risk of false positive or false negative detection.
Latest:
Quantifying Small Molecules by Mass SpectrometryThe use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration.
Latest:
On-line Monitoring of Atmospheric Inorganic Gases and Aerosols in the Southeastern and Northwestern U.S.Metrohm International
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An Investigation into the Use of Alternate Carrier Gases for the Determination of Volatile Organic Compounds in Water by GC–MSThis study focuses on United States Environmental Protection Agency (US EPA) Method 524.3 for volatile organic compounds (VOCs) in water using gas chromatography–mass spectrometry (GC–MS).
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GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic CompoundsIn most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.
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Fast and Accurate Identification of Pesticides by Direct Analysis in Real Time (DART) Ionization with Orbitrap Mass SpectrometryA method incorporating direct analysis in real time (DART) ionization and Thermo Scientific™ Orbitrap™ high-resolution mass spectrometry for rapid analysis and identification of contaminating substances in water.
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Asymmetric Flow Field-Flow Fractionation Analysis of Engineered Nanoparticles in Mouthwashes and Their Interaction with SalivaThe evaluation of the oral uptake of engineered nanoparticles (ENPs) contained in personal care products like mouthwashes is of great relevance to estimate the potential hazards and the toxicity of engineered nanomaterials (ENMs). Various experiments were performed while two commercially available mouthwash products (named M1 and M2) were selected as samples of interest. Asymmetric flow field‑flow fractionation (AF4) was chosen and optimized as the particle separation technique and two detectors were on-line coupled while dynamic light scattering (DLS) was used for evaluation and signals obtained by ultraviolet–visible (UV–vis) detection at 254 nm were used to gather additional information about the fate of the ENPs.
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Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase ChemistryUnderstanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement
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Fast and Accurate Identification of Pesticides by Direct Analysis in Real Time (DART) Ionization with Orbitrap Mass SpectrometryA method incorporating direct analysis in real time (DART) ionization and Thermo Scientific™ Orbitrap™ high-resolution mass spectrometry for rapid analysis and identification of contaminating substances in water.
