Author


Tony Taylor

Latest:

The LCGC Blog: Charged Stationary Phases in Reversed Phase HPLC

In this LCGC Blog, the author explores stationary phases that incorporate permanent or induced charges within, or alongside, hydrophobic alkyl silica type bonded ligands.


H. Lindner

Latest:

Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue Analysis

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS


The Scripps Research Institute

Latest:

Novel C18 Stationary Phase Provides Exceptional Results for Metabolite Profiling

Metabolomics, the study of small molecule metabolites that are found within a biological sample, is an emerging field of study. Progress in this field depends upon technological advancement in the fields of LC–MS and separation technology.


Andre Luciani

Latest:

Assessing the Suitability of a Green Solvent for GPC and TREF Analyses of Polyethylene

This study presents results of gel permeation chromatography (GPC) and analytical temperature rising elution fractionation (TREF) of polyethylene in butylal (dibutoxymethane, a halogen-free solvent).


Regina Römling

Latest:

Fast Antibody Titer Analysis with a New Protein A Column

Antibody related biopharmaceuticals represent one of the most dynamic segments of the pharmaceutical market. The analysis and monitoring of product titers in CHO cell cultures is one of the key tasks during development of new products. Cell line selection, optimization of expression rates, and process control need fast and efficient antibody quantification. Besides ELISA assays with known limitations with regard to reproducibility and precision, high performance liquid chromatography (HPLC) analysis using affinity chromatography is applied. This application note summarizes some performance data of a new Protein A affinity column for mAb titer analysis.







Tom Kupfer

Latest:

Application of Silica Monoliths for Bioanalysis

In this article, the application of silica monoliths with bimodal pore structure (macro and mesopores) is described for the chromatographic analysis of biomolecules including peptides, proteins, and antibodies.


Benjamin Peters

Latest:

Application of Silica Monoliths for Bioanalysis

In this article, the application of silica monoliths with bimodal pore structure (macro and mesopores) is described for the chromatographic analysis of biomolecules including peptides, proteins, and antibodies.


Peter Knoell

Latest:

Application of Silica Monoliths for Bioanalysis

In this article, the application of silica monoliths with bimodal pore structure (macro and mesopores) is described for the chromatographic analysis of biomolecules including peptides, proteins, and antibodies.


Arindam Roy

Latest:

Current Practices and Considerations for a Stability-Indicating Method in Pharmaceutical Analysis

What are the characteristics and relevant considerations for the development and validation of a stability-indicating method?


Alice Harling

Latest:

Lab-on-a-Chip GC for Environmental Research

The development of a microfabricated comprehensive GC that can be used as a portable measurement device.


Uwe Thissen

Latest:

Food Metabolomics: Fact or Fiction?

The different aspects of food metabolomics are described using tomato taste as an example.


Jeffrey Hackett

Latest:

Analysis of Psilocybin and Psilocin in Urine Using SPE and LC-Tandem Mass Spectrometry

An SPE procedure is described that isolates both psilocybin and psilocin from urine samples for LC–MS-MS analysis.


Michael Lämmerhofer

Latest:

Evaluation of Kinetic Performance of Reversed-Phase Columns for Protein Separations by Gradient Kinetic Plots

This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.


Harold M. McNair

Latest:

GC Detectors: From Thermal Conductivity to Vacuum Ultraviolet Absorption

Some might consider gas chromatography (GC) a mature technique. However, several substantial advances in GC technology in the past few years have proven that there is still room for innovation.


Editors

Latest:

Forensic Applications of Isotope-Ratio Mass Spectrometry

The isotopic profile of a material refers to the ratios of the stable isotopes of elements contained within, such as 2H/1H, 13C/12C, and 18O/16O. Biological, chemical, and physical processes cause variations in the ratios of stable isotopes; analysis of a material for its distinctive isotopic signature can thus be used to reveal information about its history. Isotope ratio mass spectrometry (IRMS) is a technique used to measure the relative abundance of isotopes in materials. Forensic investigators have used IRMS to measure a variety of materials, such as drugs, explosives, food, and human remains. In a recent web seminar, Lesley Chesson, the president of IsoForensics, Inc., explained how IRMS works and discussed the use of IRMS in forensic science, illustrating her discussion with several case examples.


Hagai Yasuor

Latest:

Trace Metabolic Profiling and Pathway Analysis of Clomazone Using LC–MS-MS and High Resolution MS

Detection, analysis, and characterization of low-abundant metabolites remain an unresolved problem in metabolic studies. In this study, we report a novel approach to address this challenge. The current methodology is derived from the predictive multiple reaction monitoring (pMRM) mode available on triple-quadrupole linear ion trap mass spectrometry (MS) systems. The pMRM mode offers the highest sensitivity among various acquisition modes for studying trace levels of metabolites of the herbicide clomazone in plants. Additionally, this method allows for the identification of positional isomers of metabolites.


R.A. Shalliker

Latest:

Improving Separation Performance and Detection Capabilities in Liquid Chromatography Using Active Flow Technology: A Review

This review will provide a brief introduction to active flow technology (AFT) with a particular focus on improvements in separation performance that can be achieved when using these columns and coupling with liquid chromatography–mass spectrometry (LC–MS).


Anna Rocco

Latest:

Forensic Drugs Analysis: A Review of Miniaturized Separation Techniques

Miniaturized separation techniques are advantageous for the analysis of illicit drugs and new psychoactive substances.


Zeineb Aturki

Latest:

Forensic Drugs Analysis: A Review of Miniaturized Separation Techniques

Miniaturized separation techniques are advantageous for the analysis of illicit drugs and new psychoactive substances.


Martin J.T. Milton

Latest:

Lab-on-a-Chip GC for Environmental Research

The development of a microfabricated comprehensive GC that can be used as a portable measurement device.


B. McKay Allred

Latest:

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.


Matthew J. Perkins

Latest:

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.


Mark A. Schure

Latest:

In Memoriam: Professor Georges A. Guiochon

On October 21, 2014, University of Tennessee Distinguished Scientist Georges Andre Guiochon succumbed to neuromuscular failure caused by post-polio syndrome. Here, we pay tribute to his remarkable career and life.


Jennifer Field

Latest:

An Assessment of Stationary Phase Selectivity in SFC

Sustainability concerns are renewing interest in SFC, often as a substitute for HPLC. With the broader application of SFC, we need a better understanding of selectivity in SFC, where stationary phase classification is not well established. This study assessed and measured the selectivity differences afforded by three prototype SFC phases.


Lois Ann Beaver (Guiochon)

Latest:

In Memoriam: Professor Georges A. Guiochon

On October 21, 2014, University of Tennessee Distinguished Scientist Georges Andre Guiochon succumbed to neuromuscular failure caused by post-polio syndrome. Here, we pay tribute to his remarkable career and life.