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Latest:
Application of Ambient Sampling Portable Mass Spectrometry Toward On-Site Screening of Clandestine Drug OperationsWorldwide trends in illicit drug use and production have shifted toward an increase in synthetic analogues and the emergence of new variations in their manufacture.
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Anomalous Retention Prediction Using Modelling Software in Gradient Reversed-Phase Liquid Chromatography: Why it Can Occur and How to Prevent ItThe success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
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Aflatoxin B1, B2, G1, G2 Food Safety Testing Prior to Automated SmartPrep® Extractor Solid-Phase ExtractionMycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply
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Analysis of Complex Pharmaceuticals by Ultrahigh-Pressure Liquid Chromatography: Case Studies and Quality Control ImplicationsExperimental results and method validation data from two analytical case studies of complex small?molecule pharmaceuticals illustrates the utility and advantages of UHPLC in high-resolution separations.
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Fast Analysis with SFCSupercritical fluid chromatography (SFC) has been used by scientists in laboratories and manufacturing facilities for several decades.
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Liquid Chromatography of Carbohydrates in Human Food and Animal Feeding StuffsThis article discusses the liquid chromatography instrumentation (columns, mobile phases, and detectors) that can be applied to determine different carbohydrates in human food and animal feeds.
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A New HILIC Column for Saccharide AnalysisDue to the highly polar nature of saccharides, the analysis of sugars is typically achieved using hydrophilic interaction chromatography (HILIC).
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HPLC UV Determination of Very High and Very Low Concentrations of Compounds in One RunAgilent Technologies
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Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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There is No Time to Waste: Low-Pressure Gas Chromatography– Mass Spectrometry is a Proven Solution for Fast, Sensitive, and Robust GC–MS AnalysisLow-pressure gas chromatography (LPGC)–MS should be the first option in many GC–MS applications to provide fast, sensitive, and robust analyses; and the installation, instrumentation, and implementation of LPGC is possible in any GC–MS system.
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Analysis of Residual Solvents in Drug ProductsGC headspace analysis is commonly used to control volatile organic components in APIs. Methods are specified in U.S. Pharmacopeia General Chapter <467>.
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Application of Ambient Sampling Portable Mass Spectrometry Toward On-Site Screening of Clandestine Drug OperationsWorldwide trends in illicit drug use and production have shifted toward an increase in synthetic analogues and the emergence of new variations in their manufacture.
Latest:
When Bad Things Happen to Good Food: Application of HPLC to Detect Food AdulterationIn this instalment, guest authors Jeff Hurst and Kendra Pfeifer from Hershey Foods explore high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), and mass spectrometry (MS) approaches being adopted to keep ahead of the food adulteration game.
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Physicochemical Methods for Vectors and Ancillary Materials in Cellular and Gene TherapiesCellular and gene therapies (CGTs) have contributed significantly to the improvement of clinical outcomes for patients in the recent years. This paper discusses a range of physicochemical methods that play an important role in the difficult characterization of viral vectors, to meet the unique needs of CGT manufacturing process development, process and product characterization, and the quality control testing of these materials.
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Analysis of Complex Pharmaceuticals by Ultrahigh-Pressure Liquid Chromatography: Case Studies and Quality Control ImplicationsExperimental results and method validation data from two analytical case studies of complex small?molecule pharmaceuticals illustrates the utility and advantages of UHPLC in high-resolution separations.
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Translations Between Differing Liquid Chromatography Formats: Advantages, Principles, and Possible PitfallsThe numerous advantages of translating gradient chromatographic methods between the differing formats of LC have been explored and discussed. While translations in principle obey well defined chromatographic theories, the authors investigate a number of potential pitfalls that may result in poor translations as exhibited by selectivity differences, changes in efficiency, and hence failure to meet resolution system suitability criteria.
Latest:
Analysis of Complex Pharmaceuticals by Ultrahigh-Pressure Liquid Chromatography: Case Studies and Quality Control ImplicationsExperimental results and method validation data from two analytical case studies of complex small?molecule pharmaceuticals illustrates the utility and advantages of UHPLC in high-resolution separations.
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HPLC for Characterization and Quality Control of Therapeutic Monoclonal AntibodiesThis instalment describes recent advances and best practices in HPLC analysis of intact monoclonal antibodies (mAbs) and their variants and fragments.
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On-Line Two-Dimensional Liquid Chromatography (2D-LC) for the Analysis of PharmaceuticalsOn-line two-dimensional liquid chromatography (2D-LC) embracing mainly comprehensive LC (LC×LC) and multiple heart-cutting LC (mLC–LC) offers new opportunities for in-depth characterization of pharmaceuticals. Reversed-phase LC × reversed-phase LC using different column chemistries and mobile phases provides good orthogonality for a wide range of applications related to small molecule drugs. Moreover, hardware configurations and software are now commercially available to perform LC×LC and mLC–LC measurements in a reproducible manner.
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Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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Screening of Drugs of Abuse Using the Velox 360 Paper Spray SystemPaper spray mass spectrometry (MS) allows for the direct analysis of pharmaceuticals, drugs of abuse, and other small molecules from blood, urine, and other biofluids (1,2).
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Positive-Negative Switching LC–MS–MS for Quantification of Pesticides in JuiceLiquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC–MS–MS) in multiple reaction monitoring (MRM) mode is widely considered as the gold standard for pesticide analysis. In this article recent developments in LC–MS–MS technology to meet increasing regulatory demands and productivity targets in quality control and contract laboratories are explored.
Latest:
Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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BioPharma Compass: A Fully Automated Solution for Characterization and QC of Intact and Digested ProteinsBioPharma Compass? is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA. This push button solution assists nonspecialist operators to generate high quality, accurate data for automatic comparison with laboratory reference standards. Automated, visual reports are then generated for each sample and important information regarding a products purity and identity can be observed at a glance. In this application note, we will apply the BioPharma Compass workflow to the QC characterization of two proteins including intact IgG1 and digested transferrin.
