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Precision of Internal Standard and External Standard Methods in High Performance Liquid ChromatographyInternal standard methods are used to improve the precision and accuracy of results where volume errors are difficult to predict and control. A systematic approach has been used to compare internal and external standard methods in high performance liquid chromatography (HPLC). The precision was determined at several different injection volumes for HPLC and ultrahigh‑pressure liquid chromatography (UHPLC), with two analyte and internal standard combinations. Precision using three methods of adding the internal standard to the analyte before final dilution was examined. The internal standard method outperformed external standard methods in all instances.
Latest:
Precision of Internal Standard and External Standard Methods in High Performance Liquid ChromatographyInternal standard methods are used to improve the precision and accuracy of results where volume errors are difficult to predict and control. A systematic approach has been used to compare internal and external standard methods in high performance liquid chromatography (HPLC). The precision was determined at several different injection volumes for HPLC and ultrahigh‑pressure liquid chromatography (UHPLC), with two analyte and internal standard combinations. Precision using three methods of adding the internal standard to the analyte before final dilution was examined. The internal standard method outperformed external standard methods in all instances.
Latest:
Precision of Internal Standard and External Standard Methods in High Performance Liquid ChromatographyInternal standard methods are used to improve the precision and accuracy of results where volume errors are difficult to predict and control. A systematic approach has been used to compare internal and external standard methods in high performance liquid chromatography (HPLC). The precision was determined at several different injection volumes for HPLC and ultrahigh‑pressure liquid chromatography (UHPLC), with two analyte and internal standard combinations. Precision using three methods of adding the internal standard to the analyte before final dilution was examined. The internal standard method outperformed external standard methods in all instances.
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Selectivity and Sensitivity Improvements for Ionizable Analytes Using High-pH-Stable Superficially Porous ParticlesA novel approach to enhancing the selectivity of ionizable compounds using superficially porous particles that are stable in a wider pH range is reported here.
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Unlocking the Power of DataThe modern analytical laboratory generates enormous amounts of data. These data are typically stored in vendor-specific, proprietary file formats
Latest:
Unlocking the Power of DataThe modern analytical laboratory generates enormous amounts of data. These data are typically stored in vendor-specific, proprietary file formats
Latest:
Unlocking the Power of DataThe modern analytical laboratory generates enormous amounts of data. These data are typically stored in vendor-specific, proprietary file formats
Latest:
Quantification of Total w-6 and w-3 Fatty Acids and w-6/w-3 Ratio in Human Serum Using GC-MSThis article demonstrates the superiority of the GC-MS approach over spectrophotometry.
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An Improved GC–MS Method for Cigarette Smoke Characterization Using a Novel Cold Trap, Dual Column, and Cryofocusing SystemCigarette smoke is a highly complex matrix and presents analytical difficulties for the analyst performing compound identification by gas chromatography analysis coupled with mass spectrometric detection (GC–MS). The development of a novel trapping system and a modified GC–MS layout (using dual chromatographic columns and cryogenic focusing devices) has improved the chromatographic separation of volatile and semi-volatile compounds found in cigarette smoke. This improvement has led to the potential to identify compounds usually masked by the solvent peak. This approach has also reduced the amount of peak overlapping by increasing the chromatographic peak capacity with the use of two capillary columns chosen for their analytical specificity.
Latest:
An Improved GC–MS Method for Cigarette Smoke Characterization Using a Novel Cold Trap, Dual Column, and Cryofocusing SystemCigarette smoke is a highly complex matrix and presents analytical difficulties for the analyst performing compound identification by gas chromatography analysis coupled with mass spectrometric detection (GC–MS). The development of a novel trapping system and a modified GC–MS layout (using dual chromatographic columns and cryogenic focusing devices) has improved the chromatographic separation of volatile and semi-volatile compounds found in cigarette smoke. This improvement has led to the potential to identify compounds usually masked by the solvent peak. This approach has also reduced the amount of peak overlapping by increasing the chromatographic peak capacity with the use of two capillary columns chosen for their analytical specificity.
Latest:
An Improved GC–MS Method for Cigarette Smoke Characterization Using a Novel Cold Trap, Dual Column, and Cryofocusing SystemCigarette smoke is a highly complex matrix and presents analytical difficulties for the analyst performing compound identification by gas chromatography analysis coupled with mass spectrometric detection (GC–MS). The development of a novel trapping system and a modified GC–MS layout (using dual chromatographic columns and cryogenic focusing devices) has improved the chromatographic separation of volatile and semi-volatile compounds found in cigarette smoke. This improvement has led to the potential to identify compounds usually masked by the solvent peak. This approach has also reduced the amount of peak overlapping by increasing the chromatographic peak capacity with the use of two capillary columns chosen for their analytical specificity.
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Benzodiazepine and Z-Drug Quantitation Using an Agilent 6430 LC-MS-MSA method was developed for the quantitation of benzodiazephines in biological samples using an Agilent 6430 Triple Quadrupole LC/MS system. The method displays excellent accuracy and precision using a weighted-quadratic calibration model.
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Benzodiazepine and Z-Drug Quantitation Using an Agilent 6430 LC-MS-MSA method was developed for the quantitation of benzodiazephines in biological samples using an Agilent 6430 Triple Quadrupole LC/MS system. The method displays excellent accuracy and precision using a weighted-quadratic calibration model.
Latest:
Benzodiazepine and Z-Drug Quantitation Using an Agilent 6430 LC-MS-MSA method was developed for the quantitation of benzodiazephines in biological samples using an Agilent 6430 Triple Quadrupole LC/MS system. The method displays excellent accuracy and precision using a weighted-quadratic calibration model.
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Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide ProfilingAn overview of mRNA-based vaccine production and a discussion on the methods that are currently in use to check the quality of these vaccines.
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A Systematic Study of Achiral Stationary Phases Using Analytes with a Molecular Diversity ModelThe authors characterized 12 SFC achiral stationary phases with 60 compounds from four chemical classes to establish guidelines for th rational selection of the optimum stationary phase for separations.
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The LCGC Blog: Moving Beyond Minimum Specs to Set Up Your Chromatography Data WorkstationIn this edition of The LCGC Blog, Katelynn Perrault and James Harynuk discuss the technological needs one faces when setting up a workstation for chromatography data analysis.
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A New Era for Lab-on-a-Chip Technologies?Miniaturization has been one of the biggest trends in recent years. This article discusses the evolution and current applications of lab?on-a-chip technology in chromatography and explores the possibility of a new market for microfluidics in separation science.
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BioPharma Compass: A Fully Automated Solution for Characterization and QC of Intact and Digested ProteinsBioPharma Compass? is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA. This push button solution assists nonspecialist operators to generate high quality, accurate data for automatic comparison with laboratory reference standards. Automated, visual reports are then generated for each sample and important information regarding a products purity and identity can be observed at a glance. In this application note, we will apply the BioPharma Compass workflow to the QC characterization of two proteins including intact IgG1 and digested transferrin.
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Time-Resolved SRM Analysis and Highly Multiplexed LC–MS-MS for Quantifying Tryptically Digested ProteinsThe inclusion of time-resolved selected reaction monitoring (SRM) functionalities into mass spectrometer control software allows large numbers of peptides to be quantified using short LC–MS-MS methods.
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Visualizing the Chemical Composition of Complex SamplesHow does one decipher and tabulate thousands of chemical formulas and relative abundances without losing the important details?
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Simplifying Mass Spectrometry Through New Ionization Technology: Application to Drugs and Clinical AnalysesA newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
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An Accurate-Mass Database for Screening Pesticide Residues in Fruits and Vegetables by Gas Chromatography–Time-of-Flight Mass SpectrometryThe main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.
Latest:
An Accurate-Mass Database for Screening Pesticide Residues in Fruits and Vegetables by Gas Chromatography–Time-of-Flight Mass SpectrometryThe main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.
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Rapid Analysis of Selected Benzodiazepines by Automated SPE–MS-MSA new method was developed and validated using automated on-line solid-phase extraction (SPE) with tandem mass spectrometry (MS). Urine samples were enzyme-hydrolyzed and diluted before detection. The validated method was applied to positive authentic urine samples to evaluate concordance with high performance liquid chromatography (HPLC)–MS-MS results.
Latest:
Rapid Analysis of Selected Benzodiazepines by Automated SPE–MS-MSA new method was developed and validated using automated on-line solid-phase extraction (SPE) with tandem mass spectrometry (MS). Urine samples were enzyme-hydrolyzed and diluted before detection. The validated method was applied to positive authentic urine samples to evaluate concordance with high performance liquid chromatography (HPLC)–MS-MS results.
Latest:
An Accurate-Mass Database for Screening Pesticide Residues in Fruits and Vegetables by Gas Chromatography–Time-of-Flight Mass SpectrometryThe main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.
Latest:
An Accurate-Mass Database for Screening Pesticide Residues in Fruits and Vegetables by Gas Chromatography–Time-of-Flight Mass SpectrometryThe main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.
